Proteome analysis of mouse brain: Two-dimensional electrophoresis profiles of tissue proteins during the course of aging

Tsugita, Akira; Kawakami, Takao; Uchida, Tatsuo; Sakai, Tomoko; Kamo, Masaharu; Matsui, Toshihiro; Watanabe, Yu; Morimasa, Tadaomi; Hosokawa, Keiichi; Toda, Tosifusa

doi:10.1002/(sici)1522-2683(20000501)21:9<1853::aid-elps1853>3.0.co;2-y

Cited by 75 publications

(34 citation statements)

References 19 publications

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“…5,2003 veloped for measurement of the expression profiles of numerous genes simultaneously and have been used to study gene expression patterns in various cancer tissues, including leukemia, colon carcinoma, breast cancer, and HCC (19,20 ). On the basis of such gene expression profiles, tumor subtypes with distinctive prognostic features have been identified (21 ).…”

Section: Discussionsupporting

confidence: 92%

Comprehensive Proteomic Profiling Identifies Serum Proteomic Signatures for Detection of Hepatocellular Carcinoma and Its Subtypes

Yip²,

et al. 2003

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Background: Detection of hepatocellular carcinoma (HCC) in patients with chronic liver disease (CLD) is difficult. We investigated the use of comprehensive proteomic profiling of sera to differentiate HCC from CLD. Methods: Proteomes in sera from 20 CLD patients with ␣-fetoprotein (AFP) <500 g/L (control group) and 38 HCC patients (disease group) were profiled by anionexchange fractionation (first dimension), two types (IMAC3 copper and WCX2) of ProteinChip ® Arrays (second dimension), and time-of-flight mass spectrometry (third dimension). Bioinformatic tests were used to identify tumor-specific proteomic features and to estimate the values of the tumor-specific proteomic features in the diagnosis of HCC. Cross-validation was performed, and we also validated the models with pooled sera from the control and disease groups, serum from a CLD patient with AFP >500 g/L, and postoperative sera from two HCC patients. Results: Among 2384 common serum proteomic features, 250 were significantly different between the HCC and CLD cases. Two-way hierarchical clustering differentiated HCC and CLD cases. Most HCC cases with advanced disease were clustered together and formed

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Section: Discussionsupporting

confidence: 92%

Comprehensive Proteomic Profiling Identifies Serum Proteomic Signatures for Detection of Hepatocellular Carcinoma and Its Subtypes

Yip²,

et al. 2003

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“…A minor fraction is associated with other functions such as transport or structure. These observations corroborate similar findings made, for example, in the analysis of the mouse brain proteome (9,10). In contrast, differential transcripts have more diverse functions (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The basic technologies for genome-wide expression analyses at the mRNA (5-7) and protein levels (8)(9)(10) are available. Transcript profiling was used to assess normal variability in gene expression levels of mouse liver, kidney, and testis (11) and to analyze changes in expression patterns during embryonic and fetal liver development (12).…”

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confidence: 97%

Identification of coexpressed gene clusters in a comparative analysis of transcriptome and proteome in mouse tissues

Mijalski

Harder

Halder

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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“…47 (2001) minal blockage than other species (Table 6). [44][45][46][47] Seventy-eight kDa glucose-regulated protein (shown in Table 1), which is identical to immunoglobulin heavy chain binding protein, is localized in the endoplasmic reticulum lumen 48) and belongs to the hsp70 family, sharing several family structural and biochemical characteristics.…”

Section: Analysis Of Phosphorylated Proteins Affected By Tcdd Exposurementioning

confidence: 99%

Proteome Analysis of the Effects of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on Murine Testicular Leydig and Sertoli Cells.

Uchida

Ohashi

Morikawa

et al. 2001

JOURNAL OF HEALTH SCIENCE

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We treated testicular cell lines Leydig TM3 and Sertoli TM4 with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and used two-dimensional electrophoresis to investigate the resulting protein alterations. Cells were cultured in a medium containing 10 -5 to 10 nM TCDD for 4 hr, under which condition viability was not affected. Protein expression was compared semi-quantitatively by silver staining, by autoradiography of [35 S] methionine-labeled proteins, and by anti-phosphotyrosine antibody. In TM3, 34 protein spots were altered by TCDD, 26 of which were increased and 8 of which were decreased; in TM4, the amount of total protein appeared to be reduced and 19 protein spots were altered by TCDD, 12 of which were increased and 7 of which were decreased. Four of these altered proteins were identified by N-terminal protein microsequencing and by a homology search against protein databases. Whereas a pyruvate dehydrogenase E1 beta subunit was decreased by TCDD exposure, ATP synthase beta chain, mitochondrial matrix protein P1 and 78 kDa glucose-regulated protein were relatively increased. The precise role of these proteins in TCDD toxicity remains to be determined, but the observed alterations suggest the proteins to be important in the effects of TCDD on testicular cells.

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Proteome analysis of mouse brain: Two-dimensional electrophoresis profiles of tissue proteins during the course of aging

Cited by 75 publications

References 19 publications

Comprehensive Proteomic Profiling Identifies Serum Proteomic Signatures for Detection of Hepatocellular Carcinoma and Its Subtypes

Comprehensive Proteomic Profiling Identifies Serum Proteomic Signatures for Detection of Hepatocellular Carcinoma and Its Subtypes

Identification of coexpressed gene clusters in a comparative analysis of transcriptome and proteome in mouse tissues

Proteome Analysis of the Effects of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on Murine Testicular Leydig and Sertoli Cells.

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