Proteome Analysis of Skin Distinguishes Acute Guttate from Chronic Plaque Psoriasis

Abstract

Psoriasis is a disease with considerable heterogeneity in clinical presentation. This is the first study using two-dimensional gel electrophoresis to compare global protein expression patterns in lesional and non-lesional skin from subjects with acute guttate psoriasis associated with streptococcal throat infection and chronic plaque psoriasis. Samples from experimentally induced contact eczema and normal skin from healthy controls were also included. Proteins with statistically significant differences in expression were used in hierarchical cluster analyses resulting in separation of the different samples into groups. Chronic plaque and guttate psoriasis samples were distinctly separated, indicating that they represent discrete phenotypes at the protein expression level. Interestingly, there was a trend in which guttate psoriasis lesions clustered closer to eczema than to chronic plaque psoriasis lesions, indicating that the duration of the inflammatory reaction may affect clustering. Several of the differentially expressed proteins were identified by mass spectrometry.

Repeated exposures to UVB induce differentiation rather than senescence of human keratinocytes lacking p16INK-4A

“…K17 is induced by interferon-c (IFNc) in keratinocytes (Jiang et al 1994), during skin inflammation linked with psoriasis (Carlen et al 2005), in skin allergy (Jiang et al 1994;Komine et al 1996) and in damaged epidermis (Bousquet and Coulombe 2002). This suggests that K17 expression may be specific of inflammatory skin conditions that might be reproduced in vitro in this model.…”

“…This suggests that K17 expression may be specific of inflammatory skin conditions that might be reproduced in vitro in this model. K6 is induced in several skin diseases including abnormal differentiation (Stoler et al 1988;Weiss et al 1984) or hyperproliferative stratified epithelium such as in psoriasis (Carlen et al 2005). K6 also participates in migration and wound healing processes (Paladini et al 1996;Wong and Coulombe 2003).…”

Ezrin, Maspin, Peroxiredoxin 2, and Heat Shock Protein 27: Potential Targets of a Streptococcal-Induced Autoimmune Response in Psoriasis

“…Maspin is restricted to epithelial cell types and is abundantly expressed in epidermis, stratified squamous epithelium of the tonsils, and the mucosal epithelium of the small intestine (39). Its expression is increased in hyperplastic psoriatic epidermis (40).…”

Primer on the human genome

“…88 Interestingly, this model demonstrated an additional requirement for activated T cells in the dermis as well as the Koebner phenomenon. 88 The global protein expression patterns of lesional skin of psoriasis 89 and atopic dermatitis 90 have also been studied. By using 2D gel electrophoresis, patterns from guttate psoriasis, plaque psoriasis, acute contact eczema, and normal skin were compared and the most differentially expressed proteins in pairwise comparisons were subjected to hierarchical clustering.…”