Proteome Analysis of Wheat Lemma

Woo, Sun Hee; Kimura, Makoto; Higa-Nishiyama, Arisa; Dohmae, Naoshi; Hamamoto, Hiroshi; Jong, Seung-Keun; Yamaguchi, Isamu

doi:10.1271/bbb.67.2486

Cited by 8 publications

(2 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ability to analyze and compare dynamic changes quantitatively in a plant proteome in response to a stimulus, using the iTRAQ approach is a significant added advantage of modern proteomics. 4 Proteome profiling has been carried out in various plant species such as Arabidopsis mitochondrion, 5 Pinus radiata, 6 Holm oak leaf, 7 wheat lemma 8 and leaf. 9 Comprehensive comparative studies of protein profiling during either development or stress responses, and functional analysis and characterization of regulatory processes are now needed to understand plant complex signaling and response mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Proteomic and Metabolic Profiling of Rice Suspension Culture Cells as a Model to Study Abscisic Acid Signaling Response Pathways in Plants

et al. 2010

View full text Add to dashboard Cite

Rice (Oryza sativa cv Taipei 309) suspension culture cells (SCCs) were used as a simple, single cell model system to gain insights into the complex abscisic acid (ABA) signaling response pathways in plants. Following system establishment involving morphological observations and transcript profiling of genes known to be ABA responsive in planta, a comprehensive proteomic and metabolomic study was performed. A total of 759 buffer-soluble proteins that included 3284 peptides categorized into 656 protein families are reported. Using iTRAQ, only 36 of these proteins showed statistically significant changes in abundance in response to ABA. In addition, a GC-MS based metabolite profiling study allowed the identification of 148 metabolites that included 25 amino acids (AAs), 45 organic acids (OAs), 35 sugars, 19 fatty acids, 2 polyamines, 4 sterols, 5 sugar acids, 4 sugar alcohols, and 9 miscellaneous compounds. Of these, only 11 (8.8%) changed in a statistically significant manner in response to ABA treatment. These studies provide important insights into plant responses to ABA at the protein and metabolite level.

show abstract

Section: Introductionmentioning

confidence: 99%

Proteomic and Metabolic Profiling of Rice Suspension Culture Cells as a Model to Study Abscisic Acid Signaling Response Pathways in Plants

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Currently many useful tools are placed on our hands to overview the whole system of life, where biologists in turn start to think big. Although a number of reviews on proteome research in plants are available (van Wijk 2001;Thiellement et al 2002;Woo et al 2002;Woo et al 2003;Islam and Hirano 2003;Rakwal and Agrawal 2003), comprehensive information on proteome research encompassing most of the plants for which genome sequence are available are scant. In this review, the enabling and challenging proteomic techniques will be reviewed and considered to hardness the disciplines whose research will be applied for the purpose of industry.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive proteome analysis using quantitative proteomic technologies

Kamal¹,

Choi²,

Cho³

et al. 2010

Journal of Plant Biotechnology

Self Cite

View full text Add to dashboard Cite

With the completion of genome sequencing of several organisms, attention has been focused to determine the function and functional network of proteins by proteome analysis. The recent techniques of proteomics have been advanced quickly so that the high-throughput and systematic analyses of cellular proteins are enabled in combination with bioinformatics tools. Furthermore, the development of proteomic techniques helps to elucidate the functions of proteins under stress or diseased condition, resulting in the discovery of biomarkers responsible for the biological stimuli. Ultimate goal of proteomics orients toward the entire proteome of life, subcellular localization, biochemical activities, and their regulation. Comprehensive analysis strategies of proteomics can be classified as three categories: (i) protein separation by 2-dimensional gel electrophoresis (2-DE) or liquid chromatography (LC), (ii) protein identification by either Edman sequencing or mass spectrometry (MS), and (iii) quanitation of proteome. Currently MS-based proteomics turns shiftly from qualitative proteome analysis by 2-DE or 2D-LC coupled with off-line matrix assisted laser desorption ionization (MALDI) and on-line electrospray ionization (ESI) MS, respectively, to quantitative proteome analysis. Some new techniques which include top-down mass spectrometry and tandem affinity purification have emerged. The in vitro quantitative proteomic techniques include differential gel electrophoresis with fluorescence dyes, protein-labeling tagging with isotope-coded affinity tag, and peptide-labeling tagging with isobaric tags for relative and absolute quantitation. In addition, stable isotope labeled amino acid can be in vivo labeled into live culture cells through metabolic incorporation. MS-based proteomics extends to detect the phosphopeptide mapping of biologically crucial protein known as one of post-translational modification. These complementary proteomic techniques contribute to not only the understanding of basic biological function but also the application to the applied sciences for industry.

show abstract

Current awareness on comparative and functional genomics

2004

Comp Funct Genom

View full text Add to dashboard Cite

In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on comparative and functional genomics. Each bibliography is divided into 16 sections. 1 Reviews & symposia; 2 General; 3 Large‐scale sequencing and mapping; 4 Genome evolution; 5 Comparative genomics; 6 Gene families and regulons; 7 Pharmacogenomics; 8 Large‐scale mutagenesis programmes; 9 Functional complementation; 10 Transcriptomics; 11 Proteomics; 12 Protein structural genomics; 13 Metabolomics; 14 Genomic approaches to development; 15 Technological advances; 16 Bioinformatics. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted

show abstract

Proteome Analysis of Wheat Lemma

Cited by 8 publications

References 18 publications

Proteomic and Metabolic Profiling of Rice Suspension Culture Cells as a Model to Study Abscisic Acid Signaling Response Pathways in Plants

Proteomic and Metabolic Profiling of Rice Suspension Culture Cells as a Model to Study Abscisic Acid Signaling Response Pathways in Plants

Comprehensive proteome analysis using quantitative proteomic technologies

Current awareness on comparative and functional genomics

Contact Info

Product

Resources

About