Proteome changes in renal cortex and medulla induced by dietary supplementation with inulin‐type fructans in growing pigs

Abstract

The aim of the study was to evaluate the influence of dietary supplementation with inulin extract from chicory root and dried chicory root on the protein profile of the renal cortex and medulla of growing pigs. The experiment was carried out on renal cortex and medulla tissue collected from 24 50‐day‐old PIC x Penarlan P76 crossbred piglets (males). Animals were divided into three dietary groups (n = 8) and fed with a control diet, diet supplemented with 2% inulin extract from chicory root and a diet supplemented with 4% dried chicory root. Kidney samples were collected after 40 days of feeding, and renal cortex and medulla proteins were separated by two‐dimensional electrophoresis. Protein identification was performed using MALDI‐TOF mass spectrometry. The diet supplemented with 2% chicory inulin induced significant expression changes of 20 and 26 protein spots in the renal cortex and medulla respectively. Supplementation with 4% dried chicory root triggered changes in the expression of 44 and 24 proteins in the renal cortex and medulla respectively. Both forms of chicory inulin‐type fructans effectively affected the expression of proteins involved in energy metabolism, heat shock proteins and other chaperones, cytoskeletal and cytoskeleton‐related proteins, as well as other proteins. Additionally, changes in transferrin abundance in both experimental groups suggested the significance of chicory fructan supplementation for iron absorption and bioavailability. In conclusion, 2% inulin extract from chicory root and 4% dried chicory root exerted a similar effect on changes in renal protein expression; however, more pronounced alterations were induced by dried chicory root. Nevertheless, further studies are needed for better understanding the mechanism underlying the effect of chicory inulin‐type fructans and their fermentation end products on the kidneys of growing pigs.

Back to the Roots: Revisiting the Use of the Fiber-Rich Cichorium intybus L. Taproots

“…For instance, in trials with companion animals it has been shown that dried whole chicory roots, as a source of prebiotic fibers, provided health benefits and contributed to longevity ( 149 , 150 ). Consequently, whole chicory roots are now also tested in studies with domestic animals for their potential as nutritive and health-promoting feed components ( 151 – 154 ).…”

Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in veterinary medicine: Recent advances (2019–present)

“…Identification of biomarkers or tracking changes in sample chemical components may often signal the presence of physiological conditions requiring treatment regimens. Table-3 summarizes recent applications of MALDI-TOF for diagnostics based on molecular typing [138][139][140][141][142][143][144][145][146][147][148][149][150][151][152][153][154]. For the first example, Riengvirodkij et al [138] used MALDI-TOF in effort to identify peptide biomarkers associated with mitral valve disease in dogs.…”

“…Feed additives for beef cattle and swine operations are continuously evaluated for affecting the operation's bottom line. Robak et al [149] have studied the effect of inulin extract and chicory root on protein expression in the renal cortex and medulla of male pigs. Matrix-assisted laser desorption ionization-time-offlight was used for protein identification.…”

Pharmacological Applications and Action Mechanisms of Phytochemicals as Alternatives to Antibiotics in Pig Production

“…Additionally, both dietary additives increase the expression of proteins related to hepatocyte protection against oxidative stress ( 44 ). Diet supplementation with dried Chicory root or inulin causes significant changes in the expression of liver cytoskeletal proteins, and alters the expression of kidney proteins engaged in energy metabolism and stress response in pigs ( 45 ). Moreover, Chicory root supplements have a potential ability to improve the liver mineral content oxidoreductive homeostasis in growing pigs ( 46 ).…”