Proteome Changes Paralleling the Olfactory Conditioning in the Forager Honey Bee and Provision of a Brain Proteomics Dataset

Sialana, Fernando J.; Menegasso, Anally Ribeiroda Silva; Šmidák, Roman; Hussein, A. M.; Zavadil, M; Rattei, Thomas; Lübec, Gert; Palma, Mário Sérgio; Lubec, Jana

doi:10.1002/pmic.201900094

“…Olfactory learning and memory behaviors of honeybees can be regulated by several proteins in the brain through proteomic analysis 40 . An in-depth proteome profile of the honeybee brain from MF and CV groups identified a total of 3,427 protein counts, 2,845 of which are both detected in MF and CV groups.…”

Section: Brain Proteomicsmentioning

confidence: 99%

Honeybee gut microbiota modulates host behaviors and neurological processes

Zhang

¹

,

Mu

²

,

Cao

³

et al. 2021

Preprint

0

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Honeybee is a highly social insect with a reach behavioral repertoire and is a versatile model for neurobiological research. The honeybee gut microbiota is composed of a limited number of bacterial phylotypes that play an important role in host health. However, it remains unclear whether the microbiota can shape brain profiles and behaviors. Here, we revealed that the gut microbiota is requisite for the olfactory learning and memory ability of honeybees and alters the level of neurotransmitters in the brain. Transcriptomic and proteomic analysis showed distinctive gene expression and protein signatures for gnotobiotic bees associated with different gut bacteria. Specifically, genes related to olfactory functions and labor division are most upregulated. Moreover, differentially spliced genes in the brains of colonized bees largely overlapped with the datasets for human autism. The circulating metabolome profiles identified that different gut species regulated specific module of metabolites in the host hemolymph. Most altered metabolites are involved in the amino acid and glycerophospholipid metabolism pathways for the production of neuroactive compounds. Finally, antibiotic treatment disturbed the gut community and the nursing behavior of worker bees under field conditions. The brain transcripts and gut metabolism was also greatly interfered in treated bees. Collectively, we demonstrate that the gut microbiota regulates honeybee behaviors, brain gene transcription, and the circulating metabolism. Our findings highlight the contributions of honeybee gut microbes in the neurological processes with striking parallels to those found in other animals, thus providing a promising model to understand the host-microbe interactions via the gut-brain axis.

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Using a toxicoproteomic approach to investigate the effects of thiamethoxam into the brain of Apis mellifera

Roat

¹

,

Santos-Pinto

²

,

Miotelo

³

et al. 2020

View full text Add to dashboard Cite

Honeybee gut microbiota modulates host behaviors and neurological processes

Zhang

¹

,

Mu

²

,

Cao

³

et al. 2020

Preprint

View full text Add to dashboard Cite

Honeybee is a highly social insect with a reach behavioral repertoire and is a versatile model for neurobiological research. The honeybee gut microbiota is composed of a limited number of bacterial phylotypes that play an important role in host health. However, it remains unclear whether the microbiota can shape brain profiles and behaviors. Here, we revealed that the gut microbiota is requisite for the olfactory learning and memory ability of honeybees and alters the level of neurotransmitters in the brain. Transcriptomic and proteomic analysis showed distinctive gene expression and protein signatures for gnotobiotic bees associated with different gut bacteria. Specifically, genes related to olfactory functions and labor division are most upregulated. Moreover, differentially spliced genes in the brains of colonized bees largely overlapped with the datasets for human autism. The circulating metabolome profiles identified that different gut species regulated specific module of metabolites in the host hemolymph. Most altered metabolites are involved in the amino acid and glycerophospholipid metabolism pathways for the production of neuroactive compounds. Finally, antibiotic treatment disturbed the gut community and the nursing behavior of worker bees under field conditions. The brain transcripts and gut metabolism was also greatly interfered in treated bees. Collectively, we demonstrate that the gut microbiota regulates honeybee behaviors, brain gene transcription, and the circulating metabolism. Our findings highlight the contributions of honeybee gut microbes in the neurological processes with striking parallels to those found in other animals, thus providing a promising model to understand the host-microbe interactions via the gut-brain axis.

show abstract

Proteome Changes Paralleling the Olfactory Conditioning in the Forager Honey Bee and Provision of a Brain Proteomics Dataset

Cited by 4 publications

References 29 publications

Honeybee gut microbiota modulates host behaviors and neurological processes

Honeybee gut microbiota modulates host behaviors and neurological processes

Using a toxicoproteomic approach to investigate the effects of thiamethoxam into the brain of Apis mellifera

Honeybee gut microbiota modulates host behaviors and neurological processes

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