2021
DOI: 10.3390/ijms22115696
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Proteome Characterization of BALF Extracellular Vesicles in Idiopathic Pulmonary Fibrosis: Unveiling Undercover Molecular Pathways

Abstract: In the longtime challenge of identifying specific, easily detectable and reliable biomarkers of IPF, BALF proteomics is providing interesting new insights into its pathogenesis. To the best of our knowledge, the present study is the first shotgun proteomic investigation of EVs isolated from BALF of IPF patients. Our main aim was to characterize the proteome of the vesicular component of BALF and to explore its individual impact on the pathogenesis of IPF. To this purpose, ultracentrifugation was chosen as the … Show more

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Cited by 14 publications
(13 citation statements)
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References 64 publications
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“…As the proteomic profile of EVs is not only related to the vesicular main components, rather it includes an extensive part of proteins strictly related to their progenitor cells, the proteomic approach by two-dimensional electrophoresis (2DE) might retain a valuable potentiality. In our recent paper about the proteomic characterization of EVs from bronchoalveolar lavage fluid (BALF) of idiopathic pulmonary fibrosis (IPF) patients, we compared the 2DE map of EVs isolated from BALF with the 2DE image of the whole biological sample, showing that the obtained protein profiles were different and characteristics [ 55 ]. As Figure 2 shows, the 2DE proteomic pattern of IPF BALF-EVs ( Figure 2 A) is visually unique and dissimilar to that of the whole BALF ( Figure 2 B), as well as to that of EVs isolated from the BALF of patients with other interstitial lung diseases (ILDs) ( Figure 2 C).…”
Section: Extracellular Vesicle As Resourceful Biological Systemsmentioning
confidence: 99%
“…As the proteomic profile of EVs is not only related to the vesicular main components, rather it includes an extensive part of proteins strictly related to their progenitor cells, the proteomic approach by two-dimensional electrophoresis (2DE) might retain a valuable potentiality. In our recent paper about the proteomic characterization of EVs from bronchoalveolar lavage fluid (BALF) of idiopathic pulmonary fibrosis (IPF) patients, we compared the 2DE map of EVs isolated from BALF with the 2DE image of the whole biological sample, showing that the obtained protein profiles were different and characteristics [ 55 ]. As Figure 2 shows, the 2DE proteomic pattern of IPF BALF-EVs ( Figure 2 A) is visually unique and dissimilar to that of the whole BALF ( Figure 2 B), as well as to that of EVs isolated from the BALF of patients with other interstitial lung diseases (ILDs) ( Figure 2 C).…”
Section: Extracellular Vesicle As Resourceful Biological Systemsmentioning
confidence: 99%
“…A growing body of literature shows that EVs are a key component in the pathobiology of IPF. Those vesicles accumulate in the lungs of patients with IPF [ 164 ], and they harbor specific cargoes of proteins and microRNA [ 164 , 165 , 166 , 167 , 168 , 169 ]. Mechanistically, fibrosis-derived EVs activate the mechanism of fibrosis, such as fibroblast proliferation and activation, by controlling developmental signaling such as Wnt or TGF-β1 [ 164 , 167 ].…”
Section: Extracellular Lipids As Important Regulators Of Fibrosis Pro...mentioning
confidence: 99%
“…EVs isolated from asthmatics transport not only specific lipids but also trigger leukotriene production in bronchial epithelial cells, which demonstrates the close link between EVs and lipid dysregulation during chronic lung diseases [ 173 ]. In IPF, EVs also carry a distinct cargo of microRNA and proteins [ 165 , 174 ]. The analysis of the proteome of the IPF-EVs shows the presence of proteins involved in lipid metabolism [ 165 ].…”
Section: Extracellular Lipids As Important Regulators Of Fibrosis Pro...mentioning
confidence: 99%
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“…BALF is the fluid that is collected through a flexible bronchoscope during bronchoalveolar lavage ( 105 ). This fluid is mainly composed of cells (including resident intrinsic trachea and alveolar cells, as well as recruited inflammatory cells), but also contains EVs or exosomes ( 106 ). It is an important diagnostic material that provides information about the immunological, infectious and inflammatory processes occurring in the alveoli ( 105 ).…”
Section: Role Of Exosomal Micrornas In Pulmonary Fibrosismentioning
confidence: 99%