Proteome characterization of the culture supernatant of Mycobacterium bovis in different growth stages

Biosynthesis of Metal and Metal Oxide Nanoparticles Using Microbial Cultures: Mechanisms, Antimicrobial Activity and Applications to Cultural Heritage

“…One study, aiming to ascertain the role and relative contribution of enzymes to the synthesis of NPs, used the cell-free denatured protein fraction of microorganism culture supernatant to prove that, despite contributing to the synthesis of NPs, enzyme catalysis is not mainly responsible or even required [ 38 ]. Moreover, studies on plant extracts that also led to the synthesis of MNPs evidenced the role of several molecules in the reduction (reducing sugars [ 27 ], flavonoids [ 27 , 28 , 34 ], proteins [ 27 ], polysaccharides [ 27 ], aldehydes [ 28 ], phenolic compounds [ 29 , 34 ] and alkaloids [ 32 ]) and stabilization (capping) (reducing sugars [ 27 ], flavonoids [ 27 , 28 , 29 ], phenolic compounds [ 28 , 34 ], alcohols [ 29 ], amines [ 29 ], alkanes [ 29 ] and alkaloids [ 32 ]) of MNPs, some of which are also present in cell-free supernatants of microorganism culture media [ 48 , 49 , 50 ]. The synthesis of MNPs using isolated compounds, such as cysteine [ 43 ], flavonols (DMY) [ 44 ], caffeic acid [ 45 ] and alginate [ 46 ], also proved that enzymatic catalysis is not mandatory for metal nanoparticle synthesis.…”