Proteome dataset of mouse macrophage cell line infected with tick-borne encephalitis virus

Русанов, А. Л.; Stepanov, Alexander A.; Zgoda, Victor G.; Kaysheva, Anna L.; Selinger, Martin; Mašková, Hana; Loginov, Dmitry S.; Štěrba, Ján; Grubhoffer, Libor; Лузгина, Н. Г.

doi:10.1016/j.dib.2019.105029

Cited by 2 publications

(1 citation statement)

References 7 publications

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“…Thus, cells exhibit many, but not all, functions and features of primary macrophages [10]. It was also demonstrated that PMJ2-R cells can be used for the investigation of an initial development phase of tick-borne encephalitis in macrophages [11].…”

Section: Introductionmentioning

confidence: 99%

Proteome Profiling of PMJ2-R and Primary Peritoneal Macrophages

Русанов

Кожин

Tikhonova

et al. 2021

IJMS

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In vitro models are often used for studying macrophage functions, including the process of phagocytosis. The application of primary macrophages has limitations associated with the individual characteristics of animals, which can lead to insufficient standardization and higher variability of the obtained results. Immortalized cell lines do not have these disadvantages, but their responses to various signals can differ from those of the living organism. In the present study, a comparative proteomic analysis of immortalized PMJ2-R cell line and primary peritoneal macrophages isolated from C57BL/6 mice was performed. A total of 4005 proteins were identified, of which 797 were quantified. Obtained results indicate significant differences in the abundances of many proteins, including essential proteins associated with the process of phagocytosis, such as Elmo1, Gsn, Hspa8, Itgb1, Ncf2, Rac2, Rack1, Sirpa, Sod1, C3, and Msr1. These findings indicate that outcomes of studies utilizing PMJ2-R cells as a model of peritoneal macrophages should be carefully validated. All MS data are deposited in ProteomeXchange with the identifier PXD022133.

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Section: Introductionmentioning

confidence: 99%

Proteome Profiling of PMJ2-R and Primary Peritoneal Macrophages

Русанов

Кожин

Tikhonova

et al. 2021

IJMS

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Plasma Proteomic Profile of Patients with Tick-Borne Encephalitis and Co-Infections

Gęgotek

Moniuszko‐Malinowska

Groth

et al. 2022

IJMS

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Despite the increasing number of patients suffering from tick-borne encephalitis (TBE), Lyme disease, and their co-infection, the mechanisms of the development of these diseases and their effects on the human body are still unknown. Therefore, the aim of this study was to evaluate the changes in the proteomic profile of human plasma induced by the development of TBE and to compare it with changes in TBE patients co-infected with other tick-borne pathogens. The results obtained by proteomic analysis using a nanoLC-Q Exactive HF mass spectrometer showed that the most highly elevated groups of proteins in the plasma of TBE patients with co-infection were involved in the pro-inflammatory response and protein degradation, while the antioxidant proteins and factors responsible for protein biosynthesis were mainly downregulated. These results were accompanied by enhanced GSH- and 4-HNE-protein adducts formation, observed in TBE and co-infected patients at a higher level than in the case of patients with only TBE. In conclusion, the differences in the proteomic profiles between patients with TBE and co-infected patients indicate that these diseases are significantly diverse and, consequently, require different treatment, which is particularly important for further research, including the development of novel diagnostics tools.

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Proteome dataset of mouse macrophage cell line infected with tick-borne encephalitis virus

Cited by 2 publications

References 7 publications

Proteome Profiling of PMJ2-R and Primary Peritoneal Macrophages

Proteome Profiling of PMJ2-R and Primary Peritoneal Macrophages

Plasma Proteomic Profile of Patients with Tick-Borne Encephalitis and Co-Infections

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