Proteome Dynamics and Physiological Responses to Short-Term Salt Stress in Brassica napus Leaves

Jia, Hongyan; Shao, Mingquan; He, Yongjun; Guan, Rongzhan; Chu, Pu; Jiang, Haidong

doi:10.1371/journal.pone.0144808

Cited by 51 publications

(46 citation statements)

References 68 publications

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“…The increases in NPQ and H d were consistent with reduced rates of carbon fixation and greater energy dissipation as heat [36]. The above results were similar to the significant inhibition of P n , G s , and WUE i caused by 200 mM NaCl in Brassica napus leaves accompanied with the reduction in Chl a, Chl b, and total Chl after three days of salt stress [37]. 24-epiBL pretreatments improved the photosynthetic rate by regulating the limitation on G m and G s given the significant reduction in C i /C a and increase in WUE i in response to 100 mM NaCl and the change in G s in response to 200 mM NaCl.…”

Section: Photosynthesissupporting

confidence: 78%

The Positive Effect of Different 24-epiBL Pretreatments on Salinity Tolerance in Robinia pseudoacacia L. Seedlings

Yue

Zhang

et al. 2018

Forests

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As a brassinosteroid (BR), 24-epibrassinolide (24-epiBL) has been widely used to enhance the resistance of plants to multiple stresses, including salinity. Black locust (Robinia pseudoacacia L.) is a common species in degraded soils. In the current study, plants were pretreated with three levels of 24-epiBL (0.21, 0.62, or 1.04 µM) by either soaking seeds during the germination phase (Sew), foliar spraying (Spw), or root dipping (Diw) at the age of 6 months. The plants were exposed to salt stress (100 and 200 mM NaCl) via automatic drip-feeding (water content~40%) for 45 days after each treatment. Increased salinity resulted in a decrease in net photosynthesis rate (P n ), stomatal conductance (G s ), intercellular:ambient CO 2 concentration ratio (C i /C a ), water-use efficiency (WUE i ), and maximum quantum yield of photosystem II (PSII) (F v /F m ). Non-photochemical quenching (NPQ) and thermal dissipation (H d ) were elevated under stress, which accompanied the reduction in the membrane steady index (MSI), water content (RWC), and pigment concentration (Chl a, Chl b, and Chl). Indicators of oxidative stress (i.e., malondialdehyde (MDA) and antioxidant enzymes (peroxidase (POD) and superoxide dismutase (SOD)) in leaves and Na + content in chloroplasts increased accompanied by a reduction in chloroplastid K + and Ca 2+ . At 200 mM NaCl, the chloroplast and thylakoid ultrastructures were severely disrupted. Exogenous 24-epiBL improved MSI, RWC, K + , and Ca 2+ content, reduced Na + levels, maintained chloroplast and thylakoid membrane structures, and enhanced the antioxidant ability in leaves. 24-epiBL also substantially alleviated stress-induced limitations of photosynthetic ability, reflected by elevated chlorophyll fluorescence, pigment levels, and P n . The positive effects of alleviating salt stress in R. pseudoacacia seedlings in terms of treatment application was Diw > Sew > Spw, and the most positive impacts were seen with 1.04 µM 24-epiBL. These results provide diverse choice for 24-epiBL usage to defend against NaCl stress of a plant.

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Section: Photosynthesissupporting

confidence: 78%

The Positive Effect of Different 24-epiBL Pretreatments on Salinity Tolerance in Robinia pseudoacacia L. Seedlings

Yue

Zhang

et al. 2018

Forests

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“…First, the accumulation of PsbS, chlorophyll a/b binding protein (CP) 24, and CP29, as well as induction of CP24 gene at 24 HAT may contribute to PsbS-protonation-dependent conformation conversion of PSII antenna system, suggesting that PsbS-dependent thermal dissipation was enhanced to minimize the potential for photo-oxidative damage under the Na 2 CO 3 treatment (Figure S8A) [31]. Consistently, CP24 and CP29 also displayed high abundances in salt-sensitive plants (Arabidopsis, oilseed rape ( Brassica napus ), and potato ( Solanum tuberosum )) and salt-tolerant plants (Indian mustard ( Brassica juncea ), mangrove ( Kandelia candel ), wild tomato ( Solanum chilense ) and Sugar beet) under salt stresses [17,32-37]. Second, the phosphorylation at Ser186 of CP24, Thr165 and Ser172 of CP26, as well as Ser95 and Thr108 of CP29 were enhanced in alkaligrass at 24 HAT (Table 1), while CP24 became dephosphorylated in NaCl-treated B. distachyon [21].…”

Section: Discussionmentioning

confidence: 79%

Na2CO3-Responsive Photosynthetic and ROS Scavenging Mechanisms in Chloroplasts of Alkaligrass Revealed by Phosphoproteomics

Suo

Zhang

Zhao

et al. 2019

Preprint

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4Alkali-salinity exerts severe osmotic, ionic and high-pH stresses to plants. To understand the 6 5 alkali-salinity responsive mechanisms underlying photosynthetic modulation and reactive 6 6 oxygen species (ROS) homeostasis, physiological and diverse quantitative proteomics 6 7 analyses of alkaligrass (Puccinellia tenuiflora) under Na 2 CO 3 stress were conducted. In 6 8 addition, Western blot, real-time PCR, and transgenic techniques were applied to validate the 6 9 proteomic results and test the functions of the Na 2 CO 3 -responsive proteins. A total of 104 and 7 0 102 Na 2 CO 3 -responsive proteins were identified in leaves and chloroplasts, respectively. In 7 1 addition, 84 Na 2 CO 3 -responsive phosphoproteins were identified, including 56 new 7 2 phosphorylation sites in 56 phosphoproteins from chloroplasts, which are crucial for the 7 3 regulation of photosynthesis, ion transport, signal transduction and energy homeostasis. A 7 4 full-length PtFBA encoding an alkaligrass chloroplastic fructose-bisphosphate aldolase (FBA) 7 5 was overexpressed in wild-type cells of cyanobacterium Synechocystis sp. Strain PCC 6803, 7 6leading to enhanced Na 2 CO 3 tolerance. All these results indicate that thermal dissipation, 7 7 state transition, cyclic electron transport, photorespiration, repair of photosystem (PS) II, PSI 7 8 activity, and ROS homeostasis were altered in response to Na 2 CO 3 stress, and they have 7 9improved our understanding of the Na 2 CO 3 -responsive mechanisms in halophytes. 8 0 8 1 8 2 Puccinellia tenuiflora 8 3 8 4 8 7 most severe abiotic stresses, limiting the productivity and geographical distribution of plants. 8 8Saline-alkali stress exerts osmotic stress and ion damage, as well as high-pH stress to plants 8 9[2]. However, little attention has been given to the sophisticated tolerance mechanisms 9 0 underlying plant response to saline-alkali (e.g. Na 2 CO 3 and NaHCO 3 ) stresses [3,4]. As the 9 1 organelle for photosynthesis, chloroplasts are extremely susceptible to saline-alkali stress [5]. 2Excessive accumulation of Na + reduces the CO 2 diffusion through stomata and mesophyll, 9 3 negatively affecting plant photosynthesis [6]. As a consequence, excessive excitation energy 9 4 causes generation of reactive oxygen species (ROS), resulting in damage to the thylakoid 9 5 membrane [6]. 9 6 4 Current high-throughput proteomic approaches are powerful to untangle the complicated 9 7 mechanisms of chloroplast development, metabolism and stress response [7−10]. More than 9 8 522 NaCl-responsive chloroplast proteins were found in different plant species, such as 9 9 tomato (Solanum lycopersicum) [11], wheat (Triticum aestivum) [12], and other plant species 1 0 0 [13−18]. The presence of these proteins indicate that the light harvesting, photosynthetic 1 0 1 electron transfer, carbon assimilation, ROS homeostasis, energy metabolism, signaling, and 4 9 5

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“…It is well known that soil salinity affects plant cells in two ways: water deficit caused by high concentrations of salt in soil leading to decreasing water uptake by roots (osmotic stress); and high accumulation of salt in the plant, which alters Na + /K + ratios as well as leading to excessive Na + and Cl − content (ion cytotoxicity; Munns and Tester, 2008; Julkowska and Testerink, 2015). Previous studies have revealed that several mechanisms such as maintenance of ion homeostasis, accumulation of compatible solutes, hormonal control, antioxidant systems, and Ca 2+ signaling are essential for plants to survive under high salinity stress (Jia et al, 2015). Based on those findings, genetic engineering and conventional breeding have been widely used to develop salt-tolerant plants.…”

Section: Introductionmentioning

confidence: 99%

The Histone Deacetylase Inhibitor Suberoylanilide Hydroxamic Acid Alleviates Salinity Stress in Cassava

et al. 2017

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Cassava (Manihot esculenta Crantz) demand has been rising because of its various applications. High salinity stress is a major environmental factor that interferes with normal plant growth and limits crop productivity. As well as genetic engineering to enhance stress tolerance, the use of small molecules is considered as an alternative methodology to modify plants with desired traits. The effectiveness of histone deacetylase (HDAC) inhibitors for increasing tolerance to salinity stress has recently been reported. Here we use the HDAC inhibitor, suberoylanilide hydroxamic acid (SAHA), to enhance tolerance to high salinity in cassava. Immunoblotting analysis reveals that SAHA treatment induces strong hyper-acetylation of histones H3 and H4 in roots, suggesting that SAHA functions as the HDAC inhibitor in cassava. Consistent with increased tolerance to salt stress under SAHA treatment, reduced Na+ content and increased K+/Na+ ratio were detected in SAHA-treated plants. Transcriptome analysis to discover mechanisms underlying salinity stress tolerance mediated through SAHA treatment reveals that SAHA enhances the expression of 421 genes in roots under normal condition, and 745 genes at 2 h and 268 genes at 24 h under both SAHA and NaCl treatment. The mRNA expression of genes, involved in phytohormone [abscisic acid (ABA), jasmonic acid (JA), ethylene, and gibberellin] biosynthesis pathways, is up-regulated after high salinity treatment in SAHA-pretreated roots. Among them, an allene oxide cyclase (MeAOC4) involved in a crucial step of JA biosynthesis is strongly up-regulated by SAHA treatment under salinity stress conditions, implying that JA pathway might contribute to increasing salinity tolerance by SAHA treatment. Our results suggest that epigenetic manipulation might enhance tolerance to high salinity stress in cassava.

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Proteome Dynamics and Physiological Responses to Short-Term Salt Stress in Brassica napus Leaves

Cited by 51 publications

References 68 publications

The Positive Effect of Different 24-epiBL Pretreatments on Salinity Tolerance in Robinia pseudoacacia L. Seedlings

The Positive Effect of Different 24-epiBL Pretreatments on Salinity Tolerance in Robinia pseudoacacia L. Seedlings

Na2CO3-Responsive Photosynthetic and ROS Scavenging Mechanisms in Chloroplasts of Alkaligrass Revealed by Phosphoproteomics

The Histone Deacetylase Inhibitor Suberoylanilide Hydroxamic Acid Alleviates Salinity Stress in Cassava

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