Proteome dynamics and physiological responses to short-term salt stress in Leymus chinensis leaves

Li, Jikai; Cui, Guowen; Hu, Guofu; Wang, Mingjun; Zhang, Pan; Qin, Ligang; Shang, Chaoqun; Zhang, Hailing; Zhu, Xiaocen

doi:10.1371/journal.pone.0183615

Cited by 27 publications

(24 citation statements)

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“…This indicated that AMF inoculation increased the chlorophyll contents, which was consistent with our proteomics results. Similar results were also found in L. chinensis by Li et al (). On the basis of the proteomic analysis, we found that several proteins involved in chlorophyll synthesis were up‐regulated after AMF inoculation under AS (AS‐AM vs. AS analysis).…”

Section: Discussionsupporting

confidence: 90%

Isobaric tags for relative and absolute quantification‐based proteomic analysis of Puccinellia tenuiflora inoculated with arbuscular mycorrhizal fungi reveal stress response mechanisms in alkali‐degraded soil

et al. 2019

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Alkali soil is a major abiotic constraint that limits plant distribution and yield in the northeast of China. Puccinellia tenuiflora is considered the most promising grass species for salt‐alkali grassland restoration. However, there is little information on the molecular mechanisms underlying how arbuscular mycorrhizal fungi (AMF) enhances P. tenuiflora stress responses in alkali‐degraded soil. In this study, AMF colonization, growth, photosynthetic pigments, and inorganic ion contents were measured. Isobaric tags for relative and absolute quantification‐based quantitative proteomic technology were employed to identify the differentially abundant proteins in P. tenuiflora seedlings with or without AMF under alkalinity stress. The results showed that AMF colonization increased seedling biomass, photosynthetic pigment contents, and K+/Na+ ratio under alkalinity stress. Moreover, a total of 598 proteins were significantly differentially regulated in P. tenuiflora seedlings after AMF inoculation under alkalinity stress compared with under alkalinity stress alone. The results showed that AMF inoculation significantly improved protein synthesis, reactive oxygen species scavenging, and nitrogen metabolism to promote the biosynthesis of osmotic substances in response to alkalinity stress. In addition, P. tenuiflora seedlings produced more energy to compensate for the energy loss caused by alkalinity stress after AMF inoculation. In conclusion, these findings provide new insights into the physiological mechanisms of the response to alkali‐degraded soil in P. tenuiflora seedlings with AMF and also clarify the role of AMF in the molecular regulation network of P. tenuiflora under alkalinity stress.

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Section: Discussionsupporting

confidence: 90%

Isobaric tags for relative and absolute quantification‐based proteomic analysis of Puccinellia tenuiflora inoculated with arbuscular mycorrhizal fungi reveal stress response mechanisms in alkali‐degraded soil

et al. 2019

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“…Under saline conditions, plants adapt themselves by osmotic adjustment, accumulation of osmoprotectants, modifications in ion transport to avoid ion toxicities, and activation of enzymes by changing gene expression (Akram et al, 2006;Athar et al, 2015). Various genes or proteins form a regulatory network to modulate such physiological or biochemical processes under abiotic stress conditions (Ibrahim et al, 2016;Li et al, 2017;Farooq et al, 2018). Under saline conditions, plants may synthesize and accumulate certain stress specific proteins which directly reveal alteration or switching on/off the possible number of salt stress related genes (Zhang et al, 2012;Li et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Various genes or proteins form a regulatory network to modulate such physiological or biochemical processes under abiotic stress conditions (Ibrahim et al, 2016;Li et al, 2017;Farooq et al, 2018). Under saline conditions, plants may synthesize and accumulate certain stress specific proteins which directly reveal alteration or switching on/off the possible number of salt stress related genes (Zhang et al, 2012;Li et al, 2017). Identification of new proteins responsible for salinity stress tolerance may help in understanding detailed mechanism of salt tolerance in plants (Li et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Under saline conditions, plants may synthesize and accumulate certain stress specific proteins which directly reveal alteration or switching on/off the possible number of salt stress related genes (Zhang et al, 2012;Li et al, 2017). Identification of new proteins responsible for salinity stress tolerance may help in understanding detailed mechanism of salt tolerance in plants (Li et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Various proteome approaches used to assess mechanism of salinity tolerance in different plant species. Advanced proteomic approaches such as SDS-PAGE complemented with LC-MS techniques may help in identification of new proteins which are responsible for salinity tolerance and/or identification of proteins which are components of various pathways that lead to salinity tolerance (Li et al, 2017). Thus, physiological responses of 13 canola cultivars to salt stress was examined.…”

Section: Introductionmentioning

confidence: 99%

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Leaf proteome analysis signified that photosynthesis and antioxidants are key indicators of salinity tolerance in canola (Brassica napus L.)

Iqbal¹,

Athar

Ibrahim

et al. 2019

PAK.J.BOT.

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Growth and yield reduction in different crops including canola is predicted to rise due to salinity stress in coming years. Understanding responses to salt stress will help in selecting and breeding salt tolerant canola cultivars. Physiological and leaf proteomic responses of 13 cultivars of canola were investigated under salt stress. In a pot experiment, three-week-old plants were grown under normal or salt stress (150 mM NaCl) for further two weeks. Out of 13 canola cultivars, cvs DGL, Dunkled, Faisal Canola and Punjab Canola were categorized as salinity stress tolerant cultivars, while cvs Bulbul-98, Oscar, Legend and Cyclone were considered as salt sensitive. Wide genotypic variations in canola cultivars have been observed in accumulation of potassium and sodium ions in the leaves and roots. Salt tolerant cultivars accumulated low Na + in their leaves than in in roots indicating limited uptake of Na + at root level with subsequent its transport to shoot. Moreover, salt tolerant cultivars had greater Na + discriminating capacity against K + . Salt tolerant cultivars were higher in leaf relative water content. Although Fv/Fm did not change in canola cultivars due to salt stress, Fv/Fo and PIABS decreased considerably in all cultivars indicating important indicators of salt stress. Salt stress increased Vj, VI, ABS/RC, TRo/RC and DIo/RC but it decreased ETo/RC which indicated that salt stress damaged the donor end of PSII (oxygen evolving complex) and reaction centers. Such adverse effects were maximal in salt sensitive cultivar Legend while minimal effects were observed in salt tolerant canola cultivars Faisal Canola, DGL and Dunkled. From comparative proteome analysis, it is obvious that 18 differentially expressed proteins in canola cultivars are mainly related with antioxidative defense system, photosynthesis and gene regulation. In addition, expression of these proteins was greater in salt tolerant cultivars. Cellular enegetics related proteins were downregulated particularly in salt sensitive cultivars due to salt stress. It is concluded that antioxidative defence system and photosynthesis are major componens of salt tolerance in canola in addition to salt exclusion. In addition, physiological studies complemented with proteomics will help in understanding detailed mechanism of salt tolerance.

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Osmoprotectants and Nonenzymatic Antioxidants in Halophytes

Surówka

Hura

2020

Handbook of Halophytes

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Salt tolerance is a complex phenomenon leading to ionic imbalance, osmotic stress, and excessive generation of reactive oxygen species (ROS). Halophytes developed a range of adaptations to tolerate disturbances in their redox homeostasis caused by high salinity alone or by its combination with other stresses. Osmoprotectants are universal molecules whose chemical structure allows them for making efficient osmotic adjustments and exerting antioxidant activity. Combining these two abilities, they become effective molecules that prevent membrane injury and stabilize proteins/enzymes under high concentrations of salts or other harmful solutes. Components of antioxidant systems together with some osmoprotectants act as ROS regulating networks. Selected compatible solutes (carbohydrates, polyols, amino acids, betaines, polyamines, phenols) (i) allow for balancing ROS level and redox status, (ii) are involved in ROSdependent signaling pathways, and (iii) activate the osmotic response network under salt stress. Osmolytes are also sources of carbon, nitrogen, sulfur, and energy for cells. Through the interaction with other biocompounds and/or their involvement in different metabolic pathways/metabolic loops, they can modulate metabolic homeostasis, especially under environmental stresses, and thus affect plant salt tolerance.

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Proteome dynamics and physiological responses to short-term salt stress in Leymus chinensis leaves

Cited by 27 publications

References 50 publications

Isobaric tags for relative and absolute quantification‐based proteomic analysis of Puccinellia tenuiflora inoculated with arbuscular mycorrhizal fungi reveal stress response mechanisms in alkali‐degraded soil

Isobaric tags for relative and absolute quantification‐based proteomic analysis of Puccinellia tenuiflora inoculated with arbuscular mycorrhizal fungi reveal stress response mechanisms in alkali‐degraded soil

Leaf proteome analysis signified that photosynthesis and antioxidants are key indicators of salinity tolerance in canola (Brassica napus L.)

Osmoprotectants and Nonenzymatic Antioxidants in Halophytes

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