Proteome mining for novel IgE‐binding proteins from the German cockroach (<i>Blattella germanica</i>) and allergen profiling of patients

Chuang, Jiing-Guang; Su, Song‐Nan; Chiang, Bor‐Luen; Lee, How‐Jing; Chow, Lu-Ping

doi:10.1002/pmic.201000348

Cited by 92 publications

(59 citation statements)

References 51 publications

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“…Prevalence of IgE antibodies was highest for rBla g 2 (54.4%) and rBla g 5 (37.4%) among the 118 sera analyzed; however, patterns of IgE antibody binding were unique to each subject. More recently, Chuang et al [6 ]also showed that IgE reactivity profiles were heterogeneous, in a group of 32 cockroach-allergic patients presumably from Taiwan. Using a panel of recombinant cockroach allergens Bla g 1, Bla g 2, Bla g 4, Bla g 5, Bla g 7, the newly identified B. germanica enolase, arginine kinase and vitellogenin, the authors demonstrated substantial differences in the prevalence of IgE reactivity to each of the allergens.…”

Section: Discussionmentioning

confidence: 99%

“…Cockroach allergens comprise at least 10 groups of distinct proteins, including Bla g 1 and Per a 1 (midgut microvilli protein homologues), Bla g 2 and Per a 2 (inactive aspartic proteases), Per a 3 (arylphorin/hemocyanin), Bla g 4 and Per a 4 (male pheromone transport lipocalins), Bla g 5 and Per a 5 (glutathione-S-transferases), Bla g 6 and Per a 6 (troponins), Bla g 7 and Per a 7 (tropomyosins), Bla g 8 (myosin light chain), Bla g 9 and Per a 9 (arginine kinases) and Per a 10 (serine protease) [5]. B. germanica enolase and vitellogenin have also been identified as novel cockroach allergens by proteomics approach [6]. In clinical practice, exclusive reactivity to either German or American cockroach extracts on skin testing is rarely observed among cockroach-allergic patients, and this is thought to be due to cross-reactivity to the homologous allergens.…”

Section: Introductionmentioning

confidence: 99%

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Efficacy of Recombinant Allergens for Diagnosis of Cockroach Allergy in Patients with Asthma and/or Rhinitis

Barbosa¹,

Santos²,

Ferriani

et al. 2013

Int Arch Allergy Immunol

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Background: Immunoglobulin E (IgE) reactivity to individual allergens among cockroach-allergic patients has revealed wide variability. The aim of this study was to assess the effectiveness of recombinant cockroach allergens for skin testing, and to determine sensitization profiles among cockroach-allergic patients living in Brazil. Methods: Fifty-seven cockroach-allergic patients with asthma and/or rhinitis were recruited. Skin testing with recombinant (r) allergens from Periplaneta americana (rPer a 1 and rPer a 7) and Blattella germanica (rBla g 2, rBla g 4 and rBla g 5) were performed at 10 μg/ml and 5 μg/ml (rPer a 1). IgE antibodies to rPer a 7 and rPer a 1 were quantitated by ELISA. Results: Of 57 patients tested, 3 (5.3%), 24 (42.1%), 4 (7%), 3 (5.3%) and 4 (7%) showed positive reactions to rPer a 1, rPer a 7, rBla g 2, rBla g 4 and rBla g 5, respectively. Twenty-eight patients (49.1%) had positive tests to at least one allergen. In keeping with skin test results, 31/57 patients (54.4%) and 5/55 patients (9%) had detectable IgE to rPer a 7 and rPer a 1, respectively. Levels of IgE to rPer a 7 were higher in patients with positive tests to rPer a 7 than those with negative tests (geometric mean 13.2 and 1.8 IU/ml, p < 0.05). There was good concordance of results of skin tests and measurements of serum IgE to rPer a 7. Conclusion: IgE reactivity to rPer a 7 (P. americana tropomyosin) was dominant among patients in Brazil. However, 50% of the patients did not present reactivity to any of the recombinant allergens tested.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Efficacy of Recombinant Allergens for Diagnosis of Cockroach Allergy in Patients with Asthma and/or Rhinitis

Barbosa¹,

Santos²,

Ferriani

et al. 2013

Int Arch Allergy Immunol

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“…The gel pieces were dehydrated with 100% ACN, dried, and subjected to in-gel digestion with sequencing-grade modified trypsin (enzyme-to-substrate ratio, 1:50; Promega) in 50 mM NH 4 HCO 3 overnight at 37°C. Peptides were extracted sequentially with 1% formic acid (FA), 60% ACN combined 0.1% FA and dried (25). To identify the protein modification by mass spectrometry (MS), tryptic-peptide preparation and data-dependent tandem MS (MS-MS) analysis were carried out as described previously (26).…”

Section: Methodsmentioning

confidence: 99%

The Ubiquitin Ligase Itch and Ubiquitination Regulate BFRF1-Mediated Nuclear Envelope Modification for Epstein-Barr Virus Maturation

Lee

Liu

Kung

et al. 2016

J Virol

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The cellular endosomal sorting complex required for transport (ESCRT) was recently found to mediate important morphogenesis processes at the nuclear envelope (NE). We previously showed that the Epstein-Barr virus (EBV) BFRF1 protein recruits the ESCRT-associated protein Alix to modulate NE structure and promote EBV nuclear egress. Here, we uncover new cellular factors and mechanisms involved in this process. BFRF1-induced NE vesicles are similar to those observed following EBV reactivation. BFRF1 is ubiquitinated, and elimination of possible ubiquitination by either lysine mutations or fusion of a deubiquitinase hampers NE-derived vesicle formation and virus maturation. While it interacts with multiple Nedd4-like ubiquitin ligases, BFRF1 preferentially binds Itch ligase. We show that Itch associates with Alix and BFRF1 and is required for BFRF1-induced NE vesicle formation. Our data demonstrate that Itch, ubiquitin, and Alix control the BFRF1-mediated modulation of the NE and EBV maturation, uncovering novel regulatory mechanisms of nuclear egress of viral nucleocapsids. IMPORTANCEThe nuclear envelope (NE) of eukaryotic cells not only serves as a transverse scaffold for cellular processes, but also as a natural barrier for most DNA viruses that assemble their nucleocapsids in the nucleus. Previously, we showed that the cellular endosomal sorting complex required for transport (ESCRT) machinery is required for the nuclear egress of EBV. Here, we further report the molecular interplay among viral BFRF1, the ESCRT adaptor Alix, and the ubiquitin ligase Itch. We found that BFRF1-induced NE vesicles are similar to those observed following EBV reactivation. The lysine residues and the ubiquitination of BFRF1 regulate the formation of BFRF1-induced NE-derived vesicles and EBV maturation. During the process, a ubiquitin ligase, Itch, preferably associates with BFRF1 and is required for BFRF1-induced NE vesicle formation. Therefore, our data indicate that Itch, ubiquitin, and Alix control the BFRF1-mediated modulation of the NE, suggesting novel regulatory mechanisms for ESCRT-mediated NE modulation. T he eukaryotic nuclear envelope (NE) is a specialized compartment composed of double lipid-bilayer membranes and an underlying proteinaceous lamina network and connected by membrane-integrating nuclear pore complexes (NPCs) that selectively regulate the nucleocytoplasmic transport of macromolecules. The NE not only provides an intact meshwork to protect the genome's integrity from cytoplasmic insults, but also serves as a natural barrier against most DNA viruses that replicate their genomes within the nucleus (1). DNA viruses thus evolve various strategies to modify the NE for efficient material transport and nuclear egress of viral nucleocapsids.Epstein-Barr virus (EBV) is a gammaherpesvirus that infects most of the human population. After primary infection, EBV becomes latent in resting B cells and can be reactivated periodically for lytic replication and virus shedding. During lytic infection, several EBV gene pr...

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“…Of note, only 1 of 28 African-American patients achieved an extended interval (Q4W) in this study. Long-interval darbepoetin regimens have also been studied extensively in patients not requiring dialysis (table 3) [101, 102]. …”

Section: Anemia Management In African-americans Through the Course Ofmentioning

confidence: 99%

The Role of Anemia Management in Improving Outcomes for African-Americans with Chronic Kidney Disease

2008

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Chronic kidney disease (CKD) is a serious threat to African-American public health. In this population CKD progresses to end-stage renal disease (ESRD) at quadruple the rate in Caucasians. Factors fueling progression to ESRD include diabetes and hypertension, which show high prevalences and accelerated renal damage in African- Americans, as well as possible nutritional, socioeconomic, and genetic factors. Anemia, a common and deleterious complication of CKD, is more prevalent and severe in African-American than Caucasian patients at each stage of the disease. Proactive management of diabetes, hypertension, anemia, and other complications throughout the course of CKD can prevent or delay disease progression and alleviate the burden of ESRD for the African-American community. Currently, African-Americans with CKD are less likely than Caucasian patients to receive anemia treatment before and after the onset of dialysis. Although African-Americans often require higher doses of erythropoiesis-stimulating agents, this may result from late treatment initiation, lower hemoglobin levels, or the presence of comorbidities such as diabetes and inflammation, although racial differences in response cannot be excluded. This review explores racial-specific challenges and potential solutions in renal anemia management to improve outcomes in African-American patients.

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Proteome mining for novel IgE‐binding proteins from the German cockroach (Blattella germanica) and allergen profiling of patients

Cited by 92 publications

References 51 publications

Efficacy of Recombinant Allergens for Diagnosis of Cockroach Allergy in Patients with Asthma and/or Rhinitis

Efficacy of Recombinant Allergens for Diagnosis of Cockroach Allergy in Patients with Asthma and/or Rhinitis

The Ubiquitin Ligase Itch and Ubiquitination Regulate BFRF1-Mediated Nuclear Envelope Modification for Epstein-Barr Virus Maturation

The Role of Anemia Management in Improving Outcomes for African-Americans with Chronic Kidney Disease

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