Proteome of Coxiella burnetii

Ihnatko, Robert; Shaw, Edward I.; Toman, Rudolf

doi:10.1007/978-94-007-4315-1_6

Cited by 8 publications

(10 citation statements)

References 134 publications

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“…Regarding the specificity of tests, some of the immunodominant proteins including the most frequently published antigens, like CBU1718 (GroEL), CBU0236 (Tuf-2), CBU1398 (SucB), CBU1290 (DnaK) and CBU0235 (FusA) are highly conserved among bacteria species (Ihnatko et al, 2012). This correlates with their housekeeping functions (Table 2).…”

Section: Sensitivity and Specificity Of Identified Immunogenic Proteinsmentioning

confidence: 83%

“…Comparison of antigenic profiles between the replicative large cell variants (LCVs) and metabolically dormant SCVs showed that the immunogenic proteins CBU1718, CBU0236, CBU0867, CBU1433, CBU0528, CBU0963, CBU1385, CBU0737 and CBU1416 are more abundant in LCVs (Ihnatko et al, 2012;Papadioti et al, 2012). Noticeably, eight of the nine antigens are involved in transcription, translation, chaperone or protein secretion activities.…”

Section: Figmentioning

confidence: 99%

“…The CBU0630 may play a role in survival and multiplication within the host cell, and it might be essential for cell entry (Ihnatko et al, 2012). Similarly, CBU1260 (OmpA) was shown to be involved in invasion as its mutation strongly inhibits C. burnetii internalization and replication within host cells (Martinez et al, 2014).…”

Section: Figmentioning

confidence: 99%

“…The following immunogenic proteins were described to have a potential as candidates for subunit vaccines: CBU0311 (P1) (Ihnatko et al, 2012), CBU1910 (Com1), CBU0092 (YbgF), CBU0612 (OmpH), CBU0891, CBU1143 (YajC) and CBU0545 (LemA) (Vigil et al, 2011). To confirm their potential as a vaccine, mouse bone marrow-derived dendritic cells were stimulated with recombinant proteins and transferred into naïve mice before C. burnetii challenge.…”

Section: Candidate Proteins For New Vaccinesmentioning

confidence: 99%

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Coxiella burnetii immunogenic proteins as a basis for new Q fever diagnostic and vaccine development

Gerlach¹,

Skultéty²,

Henning³

et al. 2017

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Summary. -Coxiella burnetii is the etiological agent of the zoonosis Q fever, which can cause an acute or a chronic, life-threatening disease in humans. It presents a highly stable cell form, which persists in the environment and is transmitted via contaminated aerosols. Ruminants are considered as the main reservoir for human infections but are usually asymptomatic. Subclinical infection in these animals and the occurrence of serologically negative shedders hamper the identification of infected animals with the currently used diagnostic techniques. This suboptimal sensitivity limits reliable identification of infected animals as well as the well-timed implementation of countermeasures. This review summarizes compounds, focusing on C. burnetii seroreactive proteins, which were discovered in recent immunoproteomic studies. We analyzed these proteins regarding their localization, function, frequency of citation, differences seen in various host species as well as sensitivity and specificity. Finally, proteins useful for the development of new diagnostic test systems as well as subunit vaccines were discussed.

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Section: Sensitivity and Specificity Of Identified Immunogenic Proteinsmentioning

confidence: 83%

Section: Figmentioning

confidence: 99%

Section: Figmentioning

confidence: 99%

Section: Candidate Proteins For New Vaccinesmentioning

confidence: 99%

See 2 more Smart Citations

Coxiella burnetii immunogenic proteins as a basis for new Q fever diagnostic and vaccine development

Gerlach¹,

Skultéty²,

Henning³

et al. 2017

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show abstract

“…A Trx peroxidase (CBU1706) is also annotated on the C. burnetii genome (Seshadri et al, 2003). This enzyme catalyzes the conversion of H 2 O 2 to H 2 O, with concurrent oxidation of Trx (Berggren et al, 2001;Ihnatko, Shaw, & Toman, 2012). Furthermore, C. burnetii expresses a DNA-binding peroxiredoxin (CBU0963) that displays peroxidase activity that is also dependent on thioredoxin/thioredoxin reductase (Hicks, Raghavan, Battisti, & Minnick, 2010), and therefore also requires an adequate supply of NADP(H).…”

Section: Discussionmentioning

confidence: 99%

SdrA, an NADP(H)‐regenerating enzyme, is crucial for Coxiella burnetii to resist oxidative stress and replicate intracellularly

Bitew

Hofmann

Souza

et al. 2020

Cellular Microbiology

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SUMMARY Coxiella burnetii, the causative agent of the zoonotic disease Q fever, is a Gram‐negative bacterium that replicates inside macrophages within a highly oxidative vacuole. Screening of a transposon mutant library suggested that sdrA, which encodes a putative short‐chain dehydrogenase, is required for intracellular replication. Short‐chain dehydrogenases are NADP(H)‐dependent oxidoreductases, and SdrA contains a predicted NADP+ binding site, suggesting it may facilitate NADP(H) regeneration by C. burnetii, a key process for surviving oxidative stress. Purified recombinant 6×His‐SdrA was able to convert NADP+ to NADP(H) in vitro. Mutation to alanine of a conserved glycine residue at position 12 within the predicted NADP binding site abolished significant enzymatic activity. Complementation of the sdrA mutant (sdrA::Tn) with plasmid‐expressed SdrA restored intracellular replication to wild‐type levels, but expressing enzymatically inactive G12A_SdrA did not. The sdrA::Tn mutant was more susceptible in vitro to oxidative stress, and treating infected host cells with L‐ascorbate, an anti‐oxidant, partially rescued the intracellular growth defect of sdrA::Tn. Finally, stable isotope labelling studies demonstrated a shift in flux through metabolic pathways in sdrA::Tn consistent with the presence of increased oxidative stress, and host cells infected with sdrA::Tn had elevated levels of reactive oxygen species compared with C. burnetii NMII.

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Contemporary diagnostics for medically relevant fastidious microorganisms belonging to the genera Anaplasma,Bartonella,Coxiella,OrientiaandRickettsia

Vaca

Dobler²,

Fischer³

et al. 2022

FEMS Microbiology Reviews

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Many of the human infectious pathogens - especially the zoonotic or vector-borne bacteria - are fastidious organisms which are difficult to cultivate because of their strong adaption to the infected host culminating in their near complete physiological dependence on this environment. These bacterial species exhibit reduced multiplication rates once they are removed from their optimal ecological niche. This fact complicates the laboratory diagnosis of the disease and hinders the detection and further characterization of the underlying organisms, e.g. at the level of their resistance to antibiotics due to their slow growth. Here, we describe the current state of microbiological diagnostics for five genera of human pathogens with a fastidious laboratory lifestyle. For Anaplasma spp., Bartonella spp., Coxiella burnetii, Orientia spp., and Rickettsia spp. we will summarize the existing diagnostic protocols, the specific limitations for implementation of novel diagnostic approaches, and the need for further optimization or expansion of the diagnostic armamentarium. We will reflect upon the diagnostic opportunities provided by new technologies including mass spectrometry and next-generation nucleic acid sequencing. Finally, we will review the (im)possibilities of rapidly developing new in vitro diagnostic tools for diseases of which the causative agents are fastidiously growing and therefore hard to detect.

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Proteome of Coxiella burnetii

Cited by 8 publications

References 134 publications

Coxiella burnetii immunogenic proteins as a basis for new Q fever diagnostic and vaccine development

Coxiella burnetii immunogenic proteins as a basis for new Q fever diagnostic and vaccine development

SdrA, an NADP(H)‐regenerating enzyme, is crucial for Coxiella burnetii to resist oxidative stress and replicate intracellularly

Contemporary diagnostics for medically relevant fastidious microorganisms belonging to the genera Anaplasma,Bartonella,Coxiella,OrientiaandRickettsia

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