Proteome of Soybean Seed Exudates Contains Plant Defense-Related Proteins Active against the Root-Knot Nematode<i>Meloidogyne incognita</i>

Rocha, Raquel O.; Morais, Janne K. S.; Oliveira, José T.A.; Oliveira, Hermógenes David de; Sousa, Daniele O.B.; Souza, Carlos Eduardo A.; Moreno, Frederico Bruno Mendes Batista; Monteiro-Moreira, Ana Cristina de Oliveira; Júnior, José Dijair Antonino de Souza; Sá, Maria Fátima Grossi de; Vasconcelos, Ilka M.

doi:10.1021/acs.jafc.5b01109

Cited by 29 publications

(16 citation statements)

References 40 publications

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“…The amount of proteins of 0.24 μgP/mg seed (3.42 ±0.29 μgP/seed) exudated from M . urundeuva seeds after soaking in 0.1 M sodium acetate buffer, pH 5.0, at 5 °C, for 24 h, followed by dialysis (cutoff: 12 kDa) against distilled water, was very low compared with that obtained for soybean seeds soaking for 18 h in 0.1 M sodium acetate buffer, pH 5.0, at 28 °C, and dialyzed (cutoff: 3.5 kDa) against distilled [ 12 ]. Besides M .…”

Section: Discussionmentioning

confidence: 99%

“…For example, the seed exudates of Cicer arietinum , which contains chitinase, chitosanase, and protease inhibitors, has antifungal activity against Fusarium oxysporum [ 10 , 11 ]. Recently, Rocha et al [ 12 ] reported that the Glycine max seed exudates contains plant-defense proteins active toward the nematode Meloidogyne incognita , a plant parasitic helminth. As chitin is a structural component of fungal cell wall [ 13 ] and eggshells of nematodes [ 14 ], bioactive compounds that degrade chitin may exert antifungal and anthelmintic effects.…”

Section: Introductionmentioning

confidence: 99%

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Myracrodruon urundeuva seed exudates proteome and anthelmintic activity against Haemonchus contortus

et al. 2018

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Seed exudates are plant-derived natural bioactive compounds consisting of a complex mixture of organic and inorganic molecules. Plant seed exudates have been poorly studied against parasite nematodes. This study was undertaken to identify proteins in the Myracrodruon urundeuva seed exudates and to assess the anthelmintic activity against Haemonchus contortus, an important parasite of small ruminants. M. urundeuva seed exudates (SEX) was obtained after immersion of seeds in sodium acetate buffer. SEX was fractionated with ammonium sulfate at 0–90% concentration to generate the ressuspended pellet (SEXF1) and the supernatant (SEXF2). SEX, SEXF1, and SEXF2 were exhaustively dialyzed against distilled water (cut-off: 12 kDa) and the protein contents determined. Mass spectrometry analyses of SEX, SEXF1, and SEXF2 were done to identify proteins and secondary metabolites. The seed exudates contained protease, protease inhibitor, peptidase, chitinase, and lipases as well as the low molecular weight secondary compounds ellagic acid and quercetin rhamnoside. SEX inhibited H. contortus larval development (LDA) (IC50 = 0.29 mg mL-1), but did not affect larval exsheathment (LEIA). On the other hand, although SEXF1 and SEXF2 inhibited H. contortus LEIA (IC50 = 1.04 and 0.93 mg mL-1, respectively), they showed even greater inhibition efficiency of H. contortus larval development (IC50 = 0.29 and 0.42 mg mL-1, respectively). To the best of our knowledge, this study is the first to show the anthelmintic activity of plant exudates against a gastrointestinal nematode. Moreover, it suggests the potential of exuded proteins as candidates to negatively interfere with H. contortus life cycle.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Myracrodruon urundeuva seed exudates proteome and anthelmintic activity against Haemonchus contortus

et al. 2018

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“…These enzymes degrade chitin, an important component of the nematode egg shell [ 41 , 42 ]. In nematodes, chitinase degrades the chitin present in the cuticles of the egg [ 35 ] and larva, inhibiting their development or leading to death [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

In vitro anthelmintic effects of Spigelia anthelmia protein fractions against Haemonchus contortus

et al. 2017

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Gastrointestinal nematodes are a significant concern for animal health and well-being, and anthelmintic treatment is mainly performed through the use of chemical products. However, bioactive compounds produced by plants have shown promise for development as novel anthelmintics. The aim of this study is to assess the anthelmintic activity of protein fractions from Spigelia anthelmia on the gastrointestinal nematode Haemonchus contortus. Plant parts were separated into leaves, stems and roots, washed with distilled water, freeze-dried and ground into a fine powder. Protein extraction was performed with sodium phosphate buffer (75 mM, pH 7.0). The extract was fractionated using ammonium sulfate (0–90%) and extensively dialyzed. The resulting fractions were named LPF (leaf protein fraction), SPF (stem protein fraction) and RPF (root protein fraction), and the protein contents and activities of the fractions were analyzed. H. contortus egg hatching (EHA), larval exsheathment inhibition (LEIA) and larval migration inhibition (LMIA) assays were performed. Proteomic analysis was conducted, and high-performance liquid chromatography (HPLC) chromatographic profiles of the fractions were established to identify proteins and possible secondary metabolites. S. anthelmia fractions inhibited H. contortus egg hatching, with LPF having the most potent effects (EC50 0.17 mg mL-1). During LEIA, SPF presented greater efficiency than the other fractions (EC50 0.25 mg mL-1). According to LMIA, the fractions from roots, stems and leaves also reduced the number of larvae, with EC50 values of 0.11, 0.14 and 0.21 mg mL-1, respectively. Protein analysis indicated the presence of plant defense proteins in the S. anthelmia fractions, including protease, protease inhibitor, chitinase and others. Conversely, secondary metabolites were absent in the S. anthemia fractions. These results suggest that S. anthelmia proteins are promising for the control of the gastrointestinal nematode H. contortus.

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“…This was regarded as the most safe and effective approach for the prevention of root-knot nematode disease [ 7 – 8 ]. Beyond the gene, Mi , the main target of researchers worldwide has become to understanding the allelopathic effect of root exudates on nematodes [ 9 – 14 ]. Root exudates can affect parasitic nematode egg hatch [ 15 – 19 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Tomato Root Exudates on Meloidogyne incognita

et al. 2016

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Plant root exudates affect root-knot nematodes egg hatch. Chemicals in root exudates can attract nematodes to the roots or result in repellence, motility inhibition or even death. However, until recently little was known about the relationship between tomato root exudates chemicals and root-knot nematodes. In this study, root exudates were extracted from three tomato rootstocks with varying levels of nematode resistance: Baliya (highly resistant, HR), RS2 (moderately resistant, MR) and L-402 (highly susceptible, T). The effects of the root exudates on Meloidogyne incognita (M. incognita) egg hatch, survival and chemotaxis of second-stage juveniles (J2) were explored. The composition of the root exudates was analysed by gas chromatography/mass spectrometry (GC/MS) prior to and following M. incognita inoculation. Four compounds in root exudates were selected for further analysis and their allopathic effect on M. incognita were investigated. Root exudates from each tomato rootstocks (HR, MR and T strains) suppressed M. incognita egg hatch and increased J2 mortality, with the highest rate being observed in the exudates from the HR plants. Exudate from HR variety also repelled M. incognita J2 while that of the susceptible plant, T, was demonstrated to be attractive. The relative amount of esters and phenol compounds in root exudates from HR and MR tomato rootstocks increased notably after inoculation. Four compounds, 2,6-Di-tert-butyl-p-cresol, L-ascorbyl 2,6-dipalmitate, dibutyl phthalate and dimethyl phthalate increased significantly after inoculation. The egg hatch of M. incognita was suppressed by each of the compound. L-ascorbyl 2,6-dipalmitate showed the most notable effect in a concentration-dependent manner. All four compounds were associated with increased J2 mortality. The greatest effect was observed with dimethyl phthalate at 2 mmol·L-1. Dibutyl phthalate was the only compound observed to repel M. incognita J2 with no effect being detected in the other compounds. Each of the four compounds were correlated with a reduction in disease index in the susceptible cultivar, T, and tomato seedlings irrigated with L-ascorbyl 2,6-dipalmitate at 2 mmol·L-1 showed the best resistance to M. incognita. Taken together, this study provided a valuable contribution to understanding the underlying mechanism of nematode resistance in tomato cultivars.

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Proteome of Soybean Seed Exudates Contains Plant Defense-Related Proteins Active against the Root-Knot NematodeMeloidogyne incognita

Cited by 29 publications

References 40 publications

Myracrodruon urundeuva seed exudates proteome and anthelmintic activity against Haemonchus contortus

Myracrodruon urundeuva seed exudates proteome and anthelmintic activity against Haemonchus contortus

In vitro anthelmintic effects of Spigelia anthelmia protein fractions against Haemonchus contortus

Effects of Tomato Root Exudates on Meloidogyne incognita

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