Proteome of tolerance fine-tuning in the human pathogen black yeast Exophiala dermatitidis

Abstract

E. dermatitidis is a pathogenic black yeast causing neurotropic infections, systemic and subcutaneous disease in a wide range of hosts, including humans. The discovery of the fungus high prevalence in man-made habitats, including sauna facilities, drinking water and dishwashers, generated concern and raised questions about the infection route. In the present work - which is the first contribution on E. dermatitidis proteome - the effect of different temperature conditions on the fungus protein pattern have been analyzed by using a gel-based approach and the temperature responsive proteins have been identified. The absence of a typical stress response following the exposure to non-optimal temperature was detected at the proteome level, along with a general reduction of the metabolic activity after exposure to cold. These results suggest that a very fine regulation of the protein expression as well as adaptations involving a basic set of stable proteins may be at the base of E. dermatitidis enormous ecological plasticity, which plays a role in the fungus distribution, also enabling the transition from natural to human habitat and to the human host.

Chemical characterization of the adhesive secretions of the salamander Plethodon shermani (Caudata, Plethodontidae)

“…In-gel tryptic digestion was carried out as previously described 62 with the modification that purified peptides were prepared for MALDI-TOF/TOF-MS. For this, the purified tryptic peptides were dissolved in 2 µL matrix solution (4 mg/mL α-Cyano-4-hydroxy cinnamic acid) and applied to stainless steel targets for analysis. Peptide Mass Fingerprinting (PMF) and peptide sequencing (MS/MS) were carried out in positive ion, reflectron mode on an UltrafleXtreme (Co. Bruker, Germany).…”

Protein functional analysis data in support of comparative proteomics of the pathogenic black yeast Exophiala dermatitidis under different temperature conditions

“…The effects of different temperature conditions on the E. dermatitidis protein expression patterns have been analyzed by using a gel-based approach and by identifying temperature responsive proteins. Culture conditions and temperature treatments were performed as described in the Journal of Proteomics paper [1] . Briefly, after growing for 7 weeks at its temperature optimum (i.e.…”

“…After protein separation using DIGE and the identification of the differentially abundant proteins, bioinformatic analyses were performed to investigate the protein biological functions, pathways and sub-cellular localization. Data source location University of Natural Resources and Life sciences, Vienna, Austria Data accessibility Data are with this article and are related to [1] …”

“…In each pie chart, semantically close terms are clustered into categories of cellular processes, being each category represented by a different color (Fig. 6) in Tesei et al [1] .…”

“…The bioinformatics analyses and data presented here provided novel insights into the cellular pathways at the base of the fungus temperature tolerance. A detailed analysis and interpretation of the data can be found in “Proteome of tolerance fine-tuning in the human pathogen black yeast Exophiala dermatitidis ” by Tesei et al (2015) [1] .…”