Proteome profiling of Epstein–Barr virus infected nasopharyngeal carcinoma cell line: Identification of potential biomarkers by comparative iTRAQ-coupled 2D LC/MS-MS analysis

Feng, Xuesong; Zhang, Jianhua; Chen, Wei Ning; Ching, Chi Bun

doi:10.1016/j.jprot.2011.01.017

Cited by 34 publications

(35 citation statements)

References 21 publications

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“…iTRAQ-labeled peptide mixtures were analyzed as described previously with a slight modification [19][20][21][22][23].The analysis was performed on combination of an Agilent 1200 nanoflow LC system (Agilent Technologies Inc., USA) and a 6530 Q-TOF mass spectrometer (Agilent Technologies Inc., USA). In the first dimension 3 μl of the combined peptide mixture was loaded onto the PolySulfoethyl A SCX column (0.3 × 50 mm, 5 μm) and was eluted stepwise by injecting salt plugs of 10 different molar concentrations of 10, 20, 30, 40, 50, 60, 80,100, 300, and 500 mM KCl solution.…”

Section: Lc-ms/ms Analysismentioning

confidence: 99%

iTRAQ-coupled 2-D LC–MS/MS analysis of cytoplasmic protein profile in Escherichia coli incubated with apidaecin IB

Zhou

Chen

2011

Journal of Proteomics

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Section: Lc-ms/ms Analysismentioning

confidence: 99%

iTRAQ-coupled 2-D LC–MS/MS analysis of cytoplasmic protein profile in Escherichia coli incubated with apidaecin IB

Zhou

Chen

2011

Journal of Proteomics

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“…In addition to the above effects, MG may impair glucose metabolism and induce energy depletion in neurons (Li et al, 2004). In rat pheochromocytoma (PC12) cells exposed to 1 mmol/L MG, apoptosis is induced via the phosphatidylinositol-3 kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR)/gammaglutamyl cysteine ligase catalytic subunit (GCLc) redox signaling pathway (Feng et al, 2011). MG also elevates the GLUT4 levels on the surface of L6 myoblasts via both increased GLUT4 translocation from the intracellular compartment and reduced GLUT4 internalization, resulting in increased glucose uptake (Engelbrecht et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Dihydromyricetin ameliorates the oxidative stress response induced by methylglyoxal via the AMPK/GLUT4 signaling pathway in PC12 cells

Jiang

Pan

et al. 2014

Brain Research Bulletin

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“…Doing so helped to establish the interactions among NPC and EBV proteins as well as to develop protein networks that elucidate their relationship in cytoskeleton formation, cell proliferation and apoptosis [17]. Feng et al employed this method in the analysis of the protein profile of an NPC cell line upon Epstein-Barr virus (EBV) infection.…”

Section: Introductionmentioning

confidence: 99%

A Quantitative Proteomics Approach in the Study of MicroRNA 181a in HepG2 Cells

Tan¹,

Habib²,

Chen³

2012

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Hepatocellular carcinoma (HCC) is currently of great concern due to its poor patient outcome despite the various therapies available. The cause of HCC usually stems from liver cirrhosis, which mainly results from Hepatitis B virus (HBV) or Hepatitis C virus (HCV) infection or environmental and genetic risk factors like alcoholism. Despite the preventive measures available now, the incidence and mortality rates are still high due to chronic infection, making it the third leading cause of cancer -related deaths in the world today and claiming approximately 600,000 lives annually. Patients are usually also diagnosed with HCC later in the stages, leading to a very poor prognosis. It is therefore imperative that a more effective treatment method is developed such that patient survival rates may be improved from current statistics of less than 50%. The role of microRNAs (miRNAs) in the regulation of gene expression and cellular development makes it an important player in cancer initiation and progression. Mir-181a has been shown to be an important miRNA involved in HCC. In this study, we propose to investigate the potential effects of miR-181a in HepG2 cells and also to explore the mechanisms in which it works in controlling cell fate. As chemotherapy is widely used in liver cancer treatment, we also study the use of miR-181a along with chemotherapy (i.e. Cisplatin). Using iTRAQ-coupled 2D LC-MS/MS analysis, we report here the study of protein profile of HepG2 cells transfected with miR-181a mimic and inhibitor separately. Three main types of cellular proteins including metabolic enzymes, protein binding and stress proteins displayed changes. The changes in the level of proteins involved in important cancer processes like cell growth were further supported by a western blot analysis. MiR-181a was subsequently found to increase HepG2 cell viability while the inhibitor displayed the opposite effect. The inhibitor also sensitized HepG2 cells to cisplatin. Cell cycle analysis showed that the inhibitor retards cell cycle progression by decreasing the proportion of cells in S and G2/M phases. Our findings therefore provide molecular evidence on the mechanism of action of miR-181a inhibitor, including its beneficial effects in inhibiting the development of HCC.

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Proteome profiling of Epstein–Barr virus infected nasopharyngeal carcinoma cell line: Identification of potential biomarkers by comparative iTRAQ-coupled 2D LC/MS-MS analysis

Cited by 34 publications

References 21 publications

iTRAQ-coupled 2-D LC–MS/MS analysis of cytoplasmic protein profile in Escherichia coli incubated with apidaecin IB

iTRAQ-coupled 2-D LC–MS/MS analysis of cytoplasmic protein profile in Escherichia coli incubated with apidaecin IB

Dihydromyricetin ameliorates the oxidative stress response induced by methylglyoxal via the AMPK/GLUT4 signaling pathway in PC12 cells

A Quantitative Proteomics Approach in the Study of MicroRNA 181a in HepG2 Cells

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