Proteomic alterations induced by ionic liquids in Aspergillus nidulans and Neurospora crassa

Martins, Isabel; Hartmann, Diego O.; Alves, Paula C.; Planchon, Sébastien; Renaut, Jenny; Leitão, Maria C.; Rebelo, Luís Paulo N.; Pereira, Cristina Silva

doi:10.1016/j.jprot.2013.09.015

Cited by 21 publications

(29 citation statements)

References 108 publications

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“…Their study revealed significant differential expression of 688 genes in response to 375 mM [C 2 C 1 Im]Cl, including up-regulation of osmoprotectant transporters and other efflux pumps, and down-regulation of certain membrane porins (Martins et al 2013).…”

Section: Elucidating Mechanisms Of Il Tolerancementioning

confidence: 99%

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Simmons

Singer

et al. 2016

Appl Microbiol Biotechnol

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Chemical and physical pretreatment of biomass is a critical step in the conversion of lignocellulose to biofuels and bioproducts. Ionic liquid (IL) pretreatment has attracted significant attention due to the unique ability of certain ILs to solubilize some or all components of the plant cell wall. However, these ILs not only inhibit enzyme activities, but also the growth and productivity of microorganisms used in downstream hydrolysis and fermentation processes.While pretreated biomass can be washed to remove residual IL and reduce inhibition, extensive washing is costly and not feasible in large-scale processes. IL tolerant microorganisms and microbial communities have been discovered from environmental samples and studies begun to elucidate mechanisms of IL tolerance. The discovery of IL tolerance in environmental microbial communities and individual microbes has lead to the proposal of molecular mechanisms of resistance. In this article, we review recent progress on discovering IL tolerant microorganisms, identifying metabolic pathways and mechanisms of tolerance, and engineering microorganisms for IL tolerance. Research in these areas will yield new approaches to overcome inhibition in lignocellulosic biomass bioconversion processes and increase opportunities for the use of ILs in biomass pretreatment.

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Section: Elucidating Mechanisms Of Il Tolerancementioning

confidence: 99%

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Simmons

Singer

et al. 2016

Appl Microbiol Biotechnol

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“…It plays major antioxidant roles fighting oxidative stress and is induced as a stress-responsive protein, e.g., in human cells exposed to Angelica sinensis extracts (Dietz et al, 2008) and in A. nidulans (AN0297) expose to either menadione (Pusztahelyi et al, 2011) or ionic liquids (Martins et al, 2013). LA also increased this stress response protein in the mycelia of P. janczewskii (spot 1304, FC = 1.9, Table 2).…”

Section: Discussionmentioning

confidence: 99%

“…Established methods were used to extract mycelial (Martins et al, 2013) and extracellular proteins (Martins et al, 2014b). Mycelial [100 mg powder mixed with 20 mg poly(vinyl)polypyrrolidone from Merck] and extracellular proteins (concentrated using 10 kDa centrifugal devices) were precipitated with cold acetone containing 10% w/v trichloroacetic acid and 60 mM DTT.…”

Section: Methodsmentioning

confidence: 99%

“…Mycelial and extracellular protein samples (80 and 50 µg, respectively) were loaded in precast 13 cm non-linear IPG strips (GE Healthcare) (pH 3–10 NL and 3–5.6 NL, respectively). Protein isoelectric focusing, SDS-PAGE, staining, image acquisition, and analysis were done as previously reported (Martins et al, 2013, 2014b). The gels were stained with flamingo dye (BioRad, USA) for image acquisition but for spot excision colloidal coomassie blue (Fluka, Switzerland) was used instead and higher quantities of protein were loaded, namely, 180 and 100 µg for mycelial and extracellular protein fractions, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Samples (0.7 µL) were then spotted on MALDI-TOF target plates (Applied Biosystems), before the deposit of 0.7 µL CHCA (7 mg/mL in ACN 50%, TFA 0.1%). Peptide mass determinations were carried out using the Applied Biosystems 5800 Proteomics Analyzer (Applied Biosystems) using established protocols (Carvalho et al, 2013; Martins et al, 2013, 2014b). Proteins were identified, with ProteinPilot, by searching against the NCBInr database (restricted to fungi taxonomy, 1,291,260 sequences) with Mascot v2.3 (Matrix Science).…”

Section: Methodsmentioning

confidence: 99%

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Proteomic Insights on the Metabolism of Penicillium janczewskii during the Biotransformation of the Plant Terpenoid Labdanolic Acid

Martins

Varela

Frija

et al. 2017

Front. Bioeng. Biotechnol.

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Plant terpenoids compose a natural source of chemodiversity of exceptional value. Many of these compounds own biological/pharmacological activity, others are regarded as unique chemical skeletons for the synthesis of derivatives with improved properties. Functional chemical modification of terpenoids through biotransformation frequently relies on the use of Ascomycota strains, but information on major cellular responses is still largely lacking. Penicillium janczewskii mediates a stereo-selective hydroxylation of labdanolic acid (LA)—terpenoid found abundantly in Cistus ladanifer—producing 3β-hydroxy-labdanolic acid with yields >90%. Herein, combined analyses of mycelial and extracellular differential proteomes demonstrated that the plant terpenoid increased stress responses, especially against oxidative stress (e.g., accumulation of superoxide dismutase) and apparently altered mitochondria functioning. One putative cytochrome P450 monooxygenase differentially accumulated in the secretome and the terpenoid bioconversion was inhibited in vivo in the presence of a P450 inhibitor. The stereo-selective hydroxylation of the plant terpenoid is likely mediated by P450 enzymes, yet its unequivocal identity remains unclear. To the best of our knowledge, this is the first time that proteomics was used to investigate how a plant terpenoid impacts the metabolism of a filamentous fungus during its efficiently biotransformation. Our findings may encourage the development of new strategies for the valorization of plant natural resources through biotechnology.

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Ligand‐Independent Activation of Aryl Hydrocarbon Receptor and Attenuation of Glutamine Levels by Natural Deep Eutectic Solvent

Denis,

Toledo,

Hakim

et al. 2023

ChemBioChem

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Natural deep eutectic solvents (NADESs) are emerging sustainable alternatives to conventional organic solvents. Beyond their role as laboratory solvents, NADESs are increasingly explored in drug delivery and as therapeutics. Their increasing applications notwithstanding, our understanding of how they interact with biomolecules at multiple levels – metabolome, proteome, and transcriptome – within human cell remain poor. Here, we deploy integrated metabolomics, proteomics, and transcriptomics to probe how NADESs perturb the molecular landscape of human cells. In a human cell line model, we found that an archetypal NADES derived from choline and geranic acid (CAGE) significantly altered the metabolome, proteome, and transcriptome. CAGE upregulated indole‐3‐lactic acid and 4‐hydroxyphenyllactic acid levels, resulting in ligand‐independent activation of aryl hydrocarbon receptor to signal the transcription of genes with implications for inflammation, immunomodulation, cell development, and chemical detoxification. Further, treating the cell line with CAGE downregulated glutamine biosynthesis, a nutrient rapidly proliferating cancer cells require. The ability of CAGE to attenuate glutamine levels is potentially relevant for cancer treatment. These findings suggest that NADESs, even when derived from natural components like choline, can indirectly modulate cell biology at multiple levels, expanding their applications beyond chemistry to biomedicine and biotechnology.

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Proteomic alterations induced by ionic liquids in Aspergillus nidulans and Neurospora crassa

Cited by 21 publications

References 108 publications

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Proteomic Insights on the Metabolism of Penicillium janczewskii during the Biotransformation of the Plant Terpenoid Labdanolic Acid

Ligand‐Independent Activation of Aryl Hydrocarbon Receptor and Attenuation of Glutamine Levels by Natural Deep Eutectic Solvent

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