Proteomic Analyses of Human Sperm Cells: Understanding the Role of Proteins and Molecular Pathways Affecting Male Reproductive Health

Agarwal, Ashok; Selvam, Manesh Kumar Panner; Baskaran, Saradha

doi:10.3390/ijms21051621

Cited by 50 publications

(38 citation statements)

References 113 publications

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“…However the identification of the sperm and amino acid concentration as an ideal biomarker is a major challenge, our results were found to be in accordance with the studies conducted by Agrawal et al (Agarwal, Selvam, and Baskaran 2020). Due to the lack of alternative testing or the need for invasive diagnostic procedures, many clinical areas in the field of male fertility are primed for the production of seminal biomarkers, our results may lead to use of prostasomes as one of the biomarker for male infertility, and all our results in accordance with Bieniek (Bieniek, Drabovich, and Lo 2016).…”

Section: Discussionsupporting

confidence: 92%

Analysis and Comparison of Human Prostasomes Amino Acid Content Variation in Normal Men and Infertile Men-A Clinical Relevance Study for Effective Diagnosis Method for Human Male Infertility

Vignesh¹

2021

revistageintec

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Aim: The main objective of this study is to compare and analyse human prostasomes amino acid content variation in normal men semen samples and infertile men semen samples for identification of clinical relevance. Materials and methods: Semen samples were collected from normal men (N=32) and from infertile men (N=32) and by following the standard world health organisation protocol semen analysis was done. Amino acid quantification was done by using amino acid analyzer. Prostasomes were separated from spermatozoa and seminal plasma by using centrifugation technique at 95000 RPM for 90 mins. Results: Independent sample T-test was carried out and shows that proline and alanine amino acids concentration (p<0.01) statistically significant compared with fertile men and infertile men. High concentration of amino acids in prostasomes were found in fertile men samples (18.09 ± 0.20 μmoles/L) when compared with infertile men samples (15.12± 0.37 μmoles/L). Conclusion: Amino acid in prostasomes plays an important role in the fertilization; the change in the concentration of amino acid in prostasomes leads to infertility of men. Here we found that the concentration of amino acids is high in fertile men when compared to infertile men which could act as an innovative diagnosis method for infertility.

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Section: Discussionsupporting

confidence: 92%

Analysis and Comparison of Human Prostasomes Amino Acid Content Variation in Normal Men and Infertile Men-A Clinical Relevance Study for Effective Diagnosis Method for Human Male Infertility

Vignesh¹

2021

revistageintec

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“…Mammalian spermatogenesis starts from undifferentiated spermatogonia, which then undergo reduction division, finally forming spermatozoa. Along with their enormous morphological transformation and genetic material shift from diploid to haploid, gene expression profiles and cell surface proteins also change significantly [1]. This progress, however, creates great challenges for the immune system in the testes, as these differentiated spermatozoa are not generated until puberty, long after the establishment and maturation of the immune system, including the tolerance to self-antigens.…”

Section: Introductionmentioning

confidence: 99%

T Lymphocytes and Testicular Immunity: A New Insight into Immune Regulation in Testes

Gong

Zeng

et al. 2020

IJMS

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The immune privilege of the testes is necessary to prevent immune attacks to gamete-specific antigens and paternal major histocompatibility complex (MHC) antigens, allowing for normal spermatogenesis. However, infection and inflammation of the male genital tract can break the immune tolerance and represent a significant cause of male infertility. Different T cell subsets have been identified in mammalian testes, which may be involved in the maintenance of immune tolerance and pathogenic immune responses in testicular infection and inflammation. We reviewed the evidence in the published literature on different T subtypes (regulatory T cells, helper T cells, cytotoxic T cells, γδ T cells, and natural killer T cells) in human and animal testes that support their regulatory roles in infertility and the orchitis pathology. While many in vitro studies have indicated the regulation potential of functional T cell subsets and their possible interaction with Sertoli cells, Leydig cells, and spermatogenesis, both under physiological and pathological processes, there have been no in situ studies to date. Nevertheless, the normal distribution and function of T cell subsets are essential for the immune privilege of the testes and intact spermatogenesis, and T cell-mediated immune response drives testicular inflammation. The distinct function of different T cell subsets in testicular homeostasis and the orchitis pathology suggests a considerable potential of targeting specific T cell subsets for therapies targeting chronic orchitis and immune infertility.

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“…This issue was particularly challenging for this work because of the process of centrosome reduction in sperm ( Manandhar et al , 2000 ; Avidor-Reiss et al , 2015 ). We could, however, enrich the samples by eliminating the sperm heads and identify more than 3406 sperm tail proteins, as previously performed in humans and in other species such as cows ( Amaral et al , 2013 ; Firat-Karalar et al , 2014 ; Cao et al , 2018 ; Agarwal et al , 2020 ). To our knowledge, this is the most complex human sperm tail proteome and also the only one that carefully examined the sperm PCM proteins.…”

Section: Discussionmentioning

confidence: 99%

The human sperm basal body is a complex centrosome important for embryo preimplantation development

Amargant

Pujol

Ferrer-Vaquer³

et al. 2021

Molecular Human Reproduction

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The mechanism of conversion of the human sperm basal body to a centrosome after fertilization, and its role in supporting human early embryogenesis, has not been directly addressed so far. Using proteomics and immunofluorescence studies we show here that the human zygote inherits a basal body enriched with centrosomal proteins from the sperm, establishing the first functional centrosome of the new organism. Injection of human sperm tails containing the basal body into human oocytes followed by parthenogenetic activation, showed that the centrosome contributes to the robustness of the early cell divisions, increasing the probability of parthenotes reaching the compaction stage. In the absence of the sperm-derived centrosome, pericentriolar (PCM) components stored in the oocyte can form de novo structures after genome activation, suggesting a tight PCM expression control in zygotes. Our results reveal that the sperm basal body is a complex organelle which converts to a centrosome after fertilization, ensuring the early steps of embryogenesis and successful compaction. However, more experiments are needed to elucidate the exact molecular mechanisms of centrosome inheritance in humans.

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Proteomic Analyses of Human Sperm Cells: Understanding the Role of Proteins and Molecular Pathways Affecting Male Reproductive Health

Cited by 50 publications

References 113 publications

Analysis and Comparison of Human Prostasomes Amino Acid Content Variation in Normal Men and Infertile Men-A Clinical Relevance Study for Effective Diagnosis Method for Human Male Infertility

Analysis and Comparison of Human Prostasomes Amino Acid Content Variation in Normal Men and Infertile Men-A Clinical Relevance Study for Effective Diagnosis Method for Human Male Infertility

T Lymphocytes and Testicular Immunity: A New Insight into Immune Regulation in Testes

The human sperm basal body is a complex centrosome important for embryo preimplantation development

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