Proteomic Analyses of the Vitreous Humour

Angi, Martina; Kalirai, Helen; Coupland, Sarah E.; Damato, Bertil; Semeraro, Francesco; Romano, Mario R.

doi:10.1155/2012/148039

Cited by 74 publications

(71 citation statements)

References 49 publications

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“…Rhegmatogenous RD occurs when the tractional forces of the vitreous on the retinal tear permit the fluid from the vitreous humor to enter the subretinal space (SRS). Vitreous fluid contains a large amount of cytokines and growth factors that stimulate the activation and the proliferation RPE and retinal glial cells [12, 18]. …”

Section: Etiopathogenesis Of Pvrmentioning

confidence: 99%

Proliferative Vitreoretinopathy after Eye Injuries: An Overexpression of Growth Factors and Cytokines Leading to a Retinal Keloid

Morescalchi

Duse

Gambicorti

et al. 2013

Mediators of Inflammation

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124

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Eye injury is a significant disabling worldwide health problem. Proliferative Vitreoretinopathy (PVR) is a common complication that develops in up to 40–60% of patients with an open-globe injury. Our knowledge about the pathogenesis of PVR has improved in the last decades. It seems that the introduction of immune cells into the vitreous, like in penetrating ocular trauma, triggers the production of growth factors and cytokines that come in contact with intra-retinal cells, like Müller cells and RPE cells. Growth factors and cytokines drive the cellular responses leading to PVR's development. Knowledge of the pathobiological and pathophysiological mechanisms involved in posttraumatic PVR is increasing the possibilities of management, and it is hoped that in the future our treatment strategies will evolve, in particular adopting a multidrug approach, and become even more effective in vision recovery. This paper reviews the current literature and clinical trial data on the pathogenesis of PVR and its correlation with ocular trauma and describes the biochemical/molecular events that will be fundamental for the development of novel treatment strategies. This literature review included PubMed articles published from 1979 through 2013. Only studies written in English were included.

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Section: Etiopathogenesis Of Pvrmentioning

confidence: 99%

Proliferative Vitreoretinopathy after Eye Injuries: An Overexpression of Growth Factors and Cytokines Leading to a Retinal Keloid

Morescalchi

Duse

Gambicorti

et al. 2013

Mediators of Inflammation

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124

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“…In a comparative proteomics study the protein composition of retina and vitreous humour was analysed showing proteins which functionally interact with each other to control oxidative stress, immune reactions and the exchange of intracellular proteins between the retina and vitreous [63]. It was demonstrated that the total protein content reported for the vitreous of patients with DR is higher than in the non-diabetic and control samples [64].…”

Section: Proteomic Methods Applied For Dr Diagnosismentioning

confidence: 99%

“…LL-37 cathelicidin is an α-helix type AMP of the tear fluid mainly produced by the corneal epithelial cells. Similar to the defensins, cathelicidin exerts various antimicrobial [102] and immunomodulatory functions [54] Complement C4b [64] Brain-specific angiogenesis inhibitor 1-associated protein 2 [70] Lysozyme C [38] VEGF [56] Complement C9 [64] Cystathionine b-synthase [70] Lipophilin A [38] Complement factor B [64] MMP-13 [70] Immunoglobulin lambda chain [38] Pigment epithelial-derived factor [64] Podocan [70] Hsp27 [76] Interstitial retinol-binding protein [64] Selenoprotein P [70] β 2 -Microglobulin [76] Inter-α trypsin inhibitor heavy chain [64] Enolase [77] [95]. In addition, it has an important role in re-epithelialization during wound healing [107].…”

Section: The Chemical Barrier Of the Tears As A Possible Source For Bmentioning

confidence: 99%

Diabetic retinopathy: Proteomic approaches to help the differential diagnosis and to understand the underlying molecular mechanisms

Csősz

Deák

Kalló

et al. 2017

Journal of Proteomics

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a b s t r a c t a r t i c l e i n f oDiabetic retinopathy is the most common diabetic eye disease and a leading cause of blindness among patients with diabetes. The appearance and the severity of the symptoms correlate with the duration of diabetes and poor blood glucose level management. Diabetic retinopathy is also categorized as a chronic low-level inflammatory disease; the high blood glucose level promotes the accumulation of the advanced glycation end products and leads to the stimulation of monocytes and macrophages. Examination of protein level alterations in tears using state-of the art proteomics techniques have identified several proteins as possible biomarkers for the different stages of the diabetic retinopathy. Some of the differentially expressed tear proteins have a role in the barrier function of tears linking the diabetic retinopathy with another eye complication of diabetes, namely the diabetic keratopathy resulting in impaired wound healing. Understanding the molecular events leading to the eye complications caused by hyperglycemia may help the identification of novel biomarkers as well as therapeutic targets in order to improve quality of life of diabetic patients. Biological significance: Diabetic retinopathy (DR), the leading cause of blindness among diabetic patients can develop without any serious symptoms therefore the early detection is crucial. Because of the increasing prevalence there is a high need for improved screening methods able to diagnose DR as soon as possible. The non-invasive collection and the relatively high protein concentration make the tear fluid a good source for biomarker discovery helping the early diagnosis. In this work we have reviewed the administration of advanced proteomics techniques used in tear biomarker studies and the identified biomarkers with potential to improve the already existing screening methods for DR detection.

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“…The differential expressions of angiopoietin-related protein 6, apolipoprotein A-I, estrogen receptor alpha, and tubulin were confirmed by Western blot analysis [75]. Since improved and more sensitive techniques for the proteome analysis are emerging, new data are expected [76]. …”

Section: Proteomicsmentioning

confidence: 99%

Candidate Genes for Proliferative Diabetic Retinopathy

Petrovič¹

2013

BioMed Research International

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Several candidate genes have been so far implicated in the pathogenesis of proliferative diabetic retinopathy (PDR) in subjects with type 2 diabetes. Since the principal pathogenetic mechanisms for diabetic retinopathy (DR) and PDR are different, the main pathogenetic mechanism in DR is increased vascular permeability, whereas in PDR the crucial pathogenetic mechanisms are fibrosis and neoangiogenesis. Due to that fact, different candidate genes are expected to be involved in the development of either DR or PDR. None of the candidate genes, however, can be fully and solely responsible for the development of PDR and for DR progression into PDR. Epigenetic mechanisms are expected to be involved in the pathogenesis of PDR as well. Gene polymorphisms responsible for PDR and epigenetic mechanisms responsible for PDR are reviewed in this paper.

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Proteomic Analyses of the Vitreous Humour

Cited by 74 publications

References 49 publications

Proliferative Vitreoretinopathy after Eye Injuries: An Overexpression of Growth Factors and Cytokines Leading to a Retinal Keloid

Proliferative Vitreoretinopathy after Eye Injuries: An Overexpression of Growth Factors and Cytokines Leading to a Retinal Keloid

Diabetic retinopathy: Proteomic approaches to help the differential diagnosis and to understand the underlying molecular mechanisms

Candidate Genes for Proliferative Diabetic Retinopathy

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