Proteomic analyses revealed the antibacterial mechanism of Aronia melanocarpa isolated anthocyanins against Escherichia coli O157: H7

A multi-omics analysis strategy reveals the molecular mechanism of the inhibition of Escherichia coli O157:H7 by anthocyanins from Aronia melanocarpa and its application

“…The method of component identification of AMAs was described in the previous report. 22 In brief, the composition of AMAs was identified by liquid chromatography-mass spectrometry (HPLC-MS). It was identified that AMAs mainly contain four anthocyanins, namely cyanidin-3- O -galactoside, cyanidin-3- O -glucoside, cyanidin-3- O -arabinoside, and cyanidin-3- O -xyloside.…”

“…In the HPLC chromatogram, the peak area ratios of the four anthocyanins were 65.368%, 3.944%, 25.119% and 4.716% respectively. 22…”

“…The proteomics method was carried out according to the previous description. 21,22 In brief, E. coli O157:H7 cells were added with lysis buffer and centrifuged to obtain the supernatant. DTT was added to the sample, and iodoacetamide was added after a water bath at 56 °C for 1 h. 30 μg of the sample was added to the ultrafiltration tube, and the waste liquid was discarded after centrifugation.…”

“…Previous reports have shown that AMAs can enter Escherichia coli O157:H7 cells through the bacillary mechanosensitive channel and affect the expression of proteins involved in oxidative stress, protein biosynthesis, DNA replication and repair and other processes. 21,22 Furthermore, metabolomics and proteomics data were integrated. First, the pathway results were classified and analyzed, including metabolism, environmental information processing, cellular processes, human diseases, and genetic information processing.…”

Combining the In Silico and In Vitro Assays to Identify Strobilanthes cusia Kuntze Bioactives against Penicillin-Resistant Streptococcus pneumoniae

“…For example, the antimicrobial activity of epigallocatechin gallate from green tea against Streptococcus suis was analyzed by the synthesis of proteomics and TEM observation to infer a possible mechanism about cell wall and membrane damage [ 16 ]. In other research, the antibacterial mechanism of anthocyanins (isolated from Aronia melanocarpa ) against Escherichia coli was also investigated by the combination of proteomics and TEM observation to discover that oxidative damage to the cells occurred after treatment [ 17 ]. A few studies employed in silico target fishing combined with experimental verification by the surface plasmon resonance assay (SPR) in order to find potential targets of a known antibacterial chemical or to screen out potential novel drugs for a known antibacterial target [ 18 ].…”