Proteomic analysis and interactions network in leaves of mycorrhizal and nonmycorrhizal sorghum plants under water deficit

Abstract

For understanding the water deficit stress mechanism in sorghum, we conducted a physiological and proteomic analysis in the leaves of Sorghum bicolor L. Moench (a drought tolerant crop model) of non-colonized and colonized plants with a consortium of arbuscular mycorrhizal fungi. Physiological results indicate that mycorrhizal fungi association enhances growth and photosynthesis in plants, under normal and water deficit conditions. 2D-electrophoresis profiles revealed 51 differentially accumulated proteins in response to water deficit, of which HPLC/MS successfully identified 49. Bioinformatics analysis of protein–protein interactions revealed the participation of different metabolic pathways in nonmycorrhizal compared to mycorrhizal sorghum plants under water deficit. In noninoculated plants, the altered proteins are related to protein synthesis and folding (50S ribosomal protein L1, 30S ribosomal protein S10, Nascent polypeptide-associated complex subunit alpha), coupled with multiple signal transduction pathways, guanine nucleotide-binding beta subunit (Rack1) and peptidyl-prolyl-cis-trans isomerase (ROC4). In contrast, in mycorrhizal plants, proteins related to energy metabolism (ATP synthase-24kDa, ATP synthase β), carbon metabolism (malate dehydrogenase, triosephosphate isomerase, sucrose-phosphatase), oxidative phosphorylation (mitochondrial-processing peptidase) and sulfur metabolism (thiosulfate/3-mercaptopyruvate sulfurtransferase) were found. Our results provide a set of proteins of different metabolic pathways involved in water deficit produced by sorghum plants alone or associated with a consortium of arbuscular mycorrhizal fungi isolated from the tropical rain forest Los Tuxtlas Veracruz, México.

Signals and Machinery for Mycorrhizae and Cereal and Oilseed Interactions towards Improved Tolerance to Environmental Stresses

“…To gain a deeper understanding of the mechanisms underlying water deficit stress, a proteomic analysis on mycorrhizal Sorghum bicolor L. plants identified 51 differentially accumulated proteins in response to water deficit stress. Notably, different metabolic pathways in AMF–sorghum leaves involved proteins related to energy (ATP synthase β, ATP synthase-24kDa) and carbon (sucrose-phosphatase, malate dehydrogenase, triosephosphate isomerase), oxidative phosphorylation (mitochondrial-processing peptidase), and sulfur (thiosulfate/3-mercaptopyruvate sulfurtransferase) metabolisms [ 143 ]. In a related study, wheat plants inoculated with AMF exhibited the down-regulation of the expression of sugar efflux transporter genes and invertases (beta-fructofuranosidase) involved in the conversion of sucrose to fructose and glucose.…”

Microbial modulation of hormone signaling, proteomic dynamics, and metabolomics in plant drought adaptation

“…Similarly, Olalde‐Portugal et al. (2020) investigated water deficit stress in sorghum leaves with and without arbuscular mycorrhizal fungal colonization. Physiological and proteomic analyses revealed enhanced growth and photosynthesis due to mycorrhizal association under both normal and water deficit conditions.…”

Proteomic Analysis Revealed Different Molecular Mechanisms of Response to PEG Stress in Drought-Sensitive and Drought-Resistant Sorghums

“…For example, drought-resistant sorghum varieties upregulate detoxification/defense-related proteins, while drought-sensitive sorghum varieties downregulate the proteins involved in metabolism to cope with water stress [ 14 , 15 , 16 , 17 ]. Furthermore, proteomic analyses indicate that mycorrhizal and nonmycorrhizal sorghum plants use different molecular mechanisms to deal with water deficit stress [ 18 ]. It is important to reveal the expression trends of differentially abundant proteins (DAPs), within and among sorghum varieties, and their biological functions in drought resistance.…”