Proteomic analysis identifies cytoskeleton‐interacting proteins as major downstream targets of altered folate status in the aorta of adult rat

Gérard, Nicolas; Chanson-Rollé, Aurélie; Rock, Edmond; Brachet, Patrick

doi:10.1002/mnfr.201400317

Cited by 2 publications

(1 citation statement)

References 60 publications

(86 reference statements)

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“…However 40% of samples are discarded as outliers and the simultaneous examination of mthfr +/and dietary folate deficiency does not allow proteomic adaptations to be attributed to either in isolation [121]. In a 2DGE-MS-based study of adult rats, aortic calmodulin (CALM1, calcium signaling) protein abundances are positively correlated with folate dose while abundances of triose phosphate isomerase (TPI1, glycolysis), transgelin (TAGLN, cytoskeleton), and glutathione s-transferase alpha 3 (GSTA3, reductive detoxification) respond inversely [122]. In an 2DGE-MS-based study of rat livers, PRDX6 and GPX1 are reported to be elevated while cofilin (CFL1) is reported to be depleted under folate deficiency [123].…”

Section: Vitamin Bmentioning

confidence: 99%

Systemic vitamin intake impacting tissue proteomes

Jeong

Vacanti

2020

Nutr Metab (Lond)

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The kinetics and localization of the reactions of metabolism are coordinated by the enzymes that catalyze them. These enzymes are controlled via a myriad of mechanisms including inhibition/activation by metabolites, compartmentalization, thermodynamics, and nutrient sensing-based transcriptional or post-translational regulation; all of which are influenced as a network by the activities of metabolic enzymes and have downstream potential to exert direct or indirect control over protein abundances. Considering many of these enzymes are active only when one or more vitamin cofactors are present; the availability of vitamin cofactors likely yields a systems-influence over tissue proteomes. Furthermore, vitamins may influence protein abundances as nuclear receptor agonists, antioxidants, substrates for post-translational modifications, molecular signal transducers, and regulators of electrolyte homeostasis. Herein, studies of vitamin intake are explored for their contribution to unraveling vitamin influence over protein expression. As a body of work, these studies establish vitamin intake as a regulator of protein abundance; with the most powerful demonstrations reporting regulation of proteins directly related to the vitamin of interest. However, as a whole, the field has not kept pace with advances in proteomic platforms and analytical methodologies, and has not moved to validate mechanisms of regulation or potential for clinical application.

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Section: Vitamin Bmentioning

confidence: 99%