Proteomic analysis in cardiovascular research

Abstract

Advances in mass spectrometry technology and bioinformatics using clinical human samples have expanded quantitative proteomics in cardiovascular research. There are two major proteomic strategies: namely, “gel-based” or “gel-free” proteomics coupled with either “top-down” or “bottom-up” mass spectrometry. Both are introduced into the proteomic analysis using plasma or serum sample targeting ‘biomarker” searches of aortic aneurysm and tissue samples, such as from the aneurysmal wall, calcific aortic valve, or myocardial tissue, investigating pathophysiological protein interactions and post-translational modifications. We summarize the proteomic studies that analyzed human samples taken during cardiovascular surgery to investigate disease processes, in order to better understand the system-wide changes behind known molecular factors and specific signaling pathways.

iTRAQ analysis of a mouse acute myocardial infarction model reveals that vitamin D binding protein promotes cardiomyocyte apoptosis after hypoxia

“…High-throughput LC MS/MS technologies have developed significantly over the last 10 years [ 11 – 13 ]. Isobaric tags for relative and absolute quantitation (iTRAQ) technology has been applied to find potential early biomarkers for Alzheimer’s disease, gastric adenocarcinoma and AMI [ 14 – 17 ].…”

Hydroxytyrosol and its complex forms (secoiridoids) modulate aorta and heart proteome in healthy rats: Potential cardio‐protective effects

“…Regarding proteomics focused on cardiovascular tissues, very few studies have been performed up to now investigating the possible protective effect on diseased animals of food bioactive compounds on the protein expression of aortic tissue testing vitamin E and omega‐3 fatty acids and heart tissue testing resveratrol . Other studies analyzing proteome on diseased cardiovascular tissues , such as calcific aortic valve or infarcted myocardium have reflected important changes in protein induced by disease . However, to the best of our knowledge, no study has reported so far the modulation of aorta and heart proteome by bioactive compounds, specifically phenolic compounds, in healthy animals to address future cardiovascular protection.…”

The Defibrillation Conundrum: New Insights into the Mechanisms of Shock-Related Myocardial Injury Sustained from a Life-Saving Therapy

“…This is concordant with some available data suggesting free-radical generation in dogs following transthoracic shocks [ 84 ]. Finally, an increase in peroxisome proliferator-activated receptor γ coactivator 1 (PGC-1) and estrogen-related receptor (ERR)-induced regulator in muscle protein 1 (Perm1), may participate in the regulation of muscle-specific transcriptional programs, such as mitochondrial biogenesis and oxidative metabolism, and adaptation of mitochondrial energy transduction pathways and oxidative metabolism [ 99 ].…”