Proteomic analysis of a murine model of lung hypoplasia induced by oligohydramnios

Najrana, Tanbir; Ahsan, Nagib; Eid, Rasha Abu; Uzun, Alper; Noble, Lelia; Tollefson, George A.; Sanchez‐Esteban, Juan

doi:10.1002/ppul.25525

Cited by 3 publications

(2 citation statements)

References 44 publications

(97 reference statements)

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“…Database search and label‐free quantitative analysis was performed as previously described [ 45 ]. Peptide spectrum matching of MS/MS spectra of each file was searched against the Uniprot Mus musculus database (TaxonID: 10090, downloaded on 02/09/2015) using the Sequest algorithm within proteome discoverer v 2.3 software (Thermo Fisher Scientific, San Jose, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Proximity proteomics reveals role of Abelson interactor 1 in the regulation of TAK1/RIPK1 signaling

et al. 2023

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Dysregulation of the adaptor protein Abelson interactor 1 (ABI1) is linked to malignant transformation. To interrogate the role of ABI1 in cancer development, we mapped the ABI1 interactome using proximity‐dependent labeling (PDL) with biotin followed by mass spectrometry. Using a novel PDL data filtering strategy, considering both peptide spectral matches and peak areas of detected peptides, we identified 212 ABI1 proximal interactors. These included WAVE2 complex components such as CYFIP1, NCKAP1, or WASF1, confirming the known role of ABI1 in the regulation of actin‐polymerization‐dependent processes. We also identified proteins associated with the TAK1‐IKK pathway, including TAK1, TAB2, and RIPK1, denoting a newly identified function of ABI1 in TAK1‐NF‐κB inflammatory signaling. Functional assays using TNFα‐stimulated, ABI1‐overexpressing or ABI1‐deficient cells showed effects on the TAK1‐NF‐kB pathway‐dependent signaling to RIPK1, with ABI1‐knockout cells being less susceptible to TNFα‐induced, RIPK1‐mediated, TAK1‐dependent apoptosis. In sum, our PDL‐based strategy enabled mapping of the ABI1 proximal interactome, thus revealing a previously unknown role of this adaptor protein in TAK1/RIPK1‐based regulation of cell death and survival.

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Section: Methodsmentioning

confidence: 99%

Proximity proteomics reveals role of Abelson interactor 1 in the regulation of TAK1/RIPK1 signaling

et al. 2023

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“…Reduced amniotic fluid volume increases the gradient between intraluminal pressure and amniotic fluid pressure. This increases the lung fluid outflow and reduces the expansion pressure in the lumen and the concentrations of various growth and maturation factors (Najrana et al, 2017(Najrana et al, , 2021. The growth factors are released when the lung tissues stretch in response to the fetal breathing movements, and stimulate the proliferation and differentiation of the epithelial cells and the production of surfactants.…”

Section: Mechanical Stress Is Critical During Pulmonary Development A...mentioning

confidence: 99%

Mechanotransduction Regulates the Interplays Between Alveolar Epithelial and Vascular Endothelial Cells in Lung

et al. 2022

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Mechanical stress plays a critical role among development, functional maturation, and pathogenesis of pulmonary tissues, especially for the alveolar epithelial cells and vascular endothelial cells located in the microenvironment established with vascular network and bronchial-alveolar network. Alveolar epithelial cells are mainly loaded by cyclic strain and air pressure tension. While vascular endothelial cells are exposed to shear stress and cyclic strain. Currently, the emerging evidences demonstrated that non-physiological mechanical forces would lead to several pulmonary diseases, including pulmonary hypertension, fibrosis, and ventilation induced lung injury. Furthermore, a series of intracellular signaling had been identified to be involved in mechanotransduction and participated in regulating the physiological homeostasis and pathophysiological process. Besides, the communications between alveolar epithelium and vascular endothelium under non-physiological stress contribute to the remodeling of the pulmonary micro-environment in collaboration, including hypoxia induced injuries, endothelial permeability impairment, extracellular matrix stiffness elevation, metabolic alternation, and inflammation activation. In this review, we aim to summarize the current understandings of mechanotransduction on the relation between mechanical forces acting on the lung and biological response in mechanical overloading related diseases. We also would like to emphasize the interplays between alveolar epithelium and vascular endothelium, providing new insights into pulmonary diseases pathogenesis, and potential targets for therapy.

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Mechanisms of mechanical stimulation in the development of respiratory system diseases

Xia,

Pan,

Wan

et al. 2024

American Journal of Physiology-Lung Cellular and Molecular Phys

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During respiration, mechanical stress can initiate biological responses that impact the respiratory system. Mechanical stress plays a crucial role in the development of the respiratory system. However, pathological mechanical stress can impact the onset and progression of respiratory diseases by influencing the extracellular matrix and cell transduction processes. In this article, we explore the mechanisms by which mechanical forces communicate with and influence cells. We outline the basic knowledge of respiratory mechanics, elucidating the important role of mechanical stimulation in influencing respiratory system development and differentiation from a microscopic perspective. We also explore the potential mechanisms of mechanical transduction in the pathogenesis and development of respiratory diseases such as asthma, lung injury, pulmonary fibrosis, and lung cancer. Finally, we look forward to new research directions in cellular mechanotransduction, aiming to provide fresh insights for future therapeutic research on respiratory diseases.

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Proteomic analysis of a murine model of lung hypoplasia induced by oligohydramnios

Cited by 3 publications

References 44 publications

Proximity proteomics reveals role of Abelson interactor 1 in the regulation of TAK1/RIPK1 signaling

Proximity proteomics reveals role of Abelson interactor 1 in the regulation of TAK1/RIPK1 signaling

Mechanotransduction Regulates the Interplays Between Alveolar Epithelial and Vascular Endothelial Cells in Lung

Mechanisms of mechanical stimulation in the development of respiratory system diseases

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