Proteomic analysis of acquired tamoxifen resistance in MCF-7 cells reveals expression signatures associated with enhanced migration

Zhou, Changhua; Zhong, Qi; Rhodes, Lyndsay V.; Townley, Ian K.; Bratton, Melyssa R.; Zhang, Qiang; Martin, Elizabeth C.; Elliott, Steven; Collins‐Burow, Bridgette M.; Burow, Matthew E.; Wang, Guangdi

doi:10.1186/bcr3144

Cited by 99 publications

(103 citation statements)

References 72 publications

(76 reference statements)

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“…3 A recent proteomics analysis of an ERα-negative, Tamoxifen-resistant MCF-7 derivative showed changes in expression of genes involved in metastasis, tumorigenesis, and ER-signaling pathways. 4 However, knowledge of the specific molecular mechanisms that cause these changes and determine the endocrine-resistance is far from complete.…”

Section: Introductionmentioning

confidence: 99%

High-density array analysis of DNA methylation in Tamoxifen-resistant breast cancer cell lines

et al. 2013

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Section: Introductionmentioning

confidence: 99%

High-density array analysis of DNA methylation in Tamoxifen-resistant breast cancer cell lines

et al. 2013

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“…The plate was then dipped in the SPS solution for 20 min followed by two 5 min washing steps in DI water with agitation. This process was repeated for either 10 or 10.5 bilayers to form (PDAC/SPS) 10 2.4. Polydimethylsiloxane (PDMS) Mold Fabrication.…”

Section: Methodsmentioning

confidence: 99%

Breast Cancer/Stromal Cells Coculture on Polyelectrolyte Films Emulates Tumor Stages and miRNA Profiles of Clinical Samples

Daverey¹,

Brown²,

Kidambi³

2015

Langmuir

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In this study, we demonstrate a method for controlling breast cancer cells adhesion on polyelectrolyte multilayer (PEM) films without the aid of adhesive proteins/ ligands to study the role of tumor and stromal cell interaction on cancer biology. Numerous studies have explored engineering coculture of tumor and stromal cells predominantly using transwell coculture of stromal cells cultured onto coverslips that were subsequently added to tumor cell cultures. However, these systems imposed an artificial boundary that precluded cell−cell interactions. To our knowledge, this is the f irst demonstration of patterned coculture of tumor cells and stromal cells that captures the temporal changes in the miRNA signature as the breast tumor develops through various stages. In our study we used synthetic polymers, namely poly(diallyldimethylammonium chloride) (PDAC) and sulfonated poly(styrene) (SPS), as the polycation and polyanion, respectively, to build PEMs. Breast cancer cells attached and spread preferentially on SPS surfaces while stromal cells attached to both SPS and PDAC surfaces. SPS patterns were formed on PEM surfaces, by either capillary force lithography (CFL) of SPS onto PDAC surfaces or vice versa, to obtain patterns of breast cancer cells and patterned cocultures of breast cancer and stromal cells. In this study, we utilized cancer cells derived from two different tumor stages and two different stromal cells to effectively model a heterogeneous tumor microenvironment and emulate various tumor stages. The coculture model mimics the proliferative index (Ki67 expression) and tumor aggressiveness (HER-2 expression) akin to those observed in clinical tumor samples. We also demonstrated that our patterned coculture model captures the temporal changes in the miRNA-21 and miRNA-34 signature as the breast tumor develops through various stages. The engineered coculture platform lays groundwork toward precision medicine wherein patient-derived tumor cells can be incorporated within our in vitro models to identify potential pathways and drug treatment regimens for individual patients.

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“…4 However, knowledge of the specific molecular mechanisms that cause these changes and determine the endocrine-resistance is far from complete.…”

Section: Introductionmentioning

confidence: 99%

Epigenetic Control of Tamoxifen-Resistant Breast Cancer

Williams¹

2014

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show abstract

Proteomic analysis of acquired tamoxifen resistance in MCF-7 cells reveals expression signatures associated with enhanced migration

Cited by 99 publications

References 72 publications

High-density array analysis of DNA methylation in Tamoxifen-resistant breast cancer cell lines

High-density array analysis of DNA methylation in Tamoxifen-resistant breast cancer cell lines

Breast Cancer/Stromal Cells Coculture on Polyelectrolyte Films Emulates Tumor Stages and miRNA Profiles of Clinical Samples

Epigenetic Control of Tamoxifen-Resistant Breast Cancer

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