Proteomic analysis of altered proteins in lymphoid organ of yellow head virus infected Penaeus monodon

Differentially expressed genes in Penaeus monodon hemocytes following infection with yellow head virus

“…Crustacean transglutaminase is an enzyme that cross-links hemolymph clots to prevent excess blood loss from a wound and invasion of micro-organisms into the wound (15); its down-regulation at a late stage of YHV infection in our study was in accordance with that reported in WSSV-infected Chinese shrimp (16). Using proteomic approaches, Bourchookarn et al (6) found down-regulation of hemolymph clottable protein and up-regulation of transglutaminase upon YHV infection.…”

“…However, in this study, YHV infection of P. monodon did not affect the expression level of several calcium-dependent genes including calnexin, calponin, calreticulin and calcium-sensitive chloride channel, in contrast to the reported up-regulation of calreticulin in WSSV-infected shrimp at http://bmbreports.org BMB reports both the mRNA and protein levels (3,18). In addition, no calcium-dependent protein was identified by comparative proteomic analysis between normal and YHV-infected shrimp (6). The up-regulation of several calcium-related genes in WSSV infection may be a compensating reaction of the host genes.…”

“…In contrast, the responses of shrimp to YHV infection at the molecular level are currently poorly described. WSSV is an enveloped, ellipsoid and large double-stranded DNA virus (5), while YHV is an enveloped, rod-shaped and single-stranded RNA virus (6). As YHV contains a single-stranded RNA genome, the molecular basis for shrimp response to YHV might be somewhat different than that to the DNA virus WSSV.…”

Proteomic analysis of differentially expressed proteins in lymphoid organ of Fenneropenaeus chinensis response to Vibrio anguillarum stimulation

“…A decrease of soluble actin abundance in the lymphoid organ of shrimp challenged by V. anguillarum might be caused by the formation of microfilaments, which is the polymerized form of actin [39]. It was reported that some cytoskeleton proteins including beta-actin, actin-2 and fast tropomyosin decreased in the lymphoid organ of YHV-infected P. monodon [40]. Microtubules are assembled from the dimers of a-and b-tubulin, which serve as structural components within cells and are involved in many cellular processes including mitosis, cytokinesis, and vesicular transport.…”

Differential expression of proteins in the gills of Litopenaeus vannamei infected with white spot syndrome virus

“…Thus, knowing the probable infection, biosafety measures can be adopted early and applied for future development of antiviral defenses, because the animal needs a fast response in the beginning of infection to combat the virus with efficacy and minimize the impacts of infection in shrimp farming. In recent years, studies on shrimp immunity at the molecular level has increased rapidly for both cellular and humoral responses, in order to elucidate host immune response mechanisms to viral infection (Bourchookarn et al 2008;Robalino et al 2009;Chai et al 2010;Zhang et al 2010;Somboonwiwat et al 2010).…”