Proteomic analysis of ampicillin‐resistant oral <i>Fusobacterium nucleatum</i>

Al‐Haroni, Mohammed; Skaug, Nils; Bakken, Vidar; Cash, Phillip

doi:10.1111/j.1399-302x.2007.00387.x

Cited by 34 publications

(33 citation statements)

References 39 publications

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“…In fact, regardless of cell motivation, RNA degradation is often seen as a good target for bacteria control, and it is the focus of drug development. Enolase also was found upregulated in an ampicillin-resistant Fusobacterium nucleatum strain (2). Enolase is certainly involved in cellular processes such as energy production, RNA degradation, and plasminogen/plasmin system, and it is presumed to be important in resistance to these antimicrobial agents.…”

Section: Discussionmentioning

confidence: 97%

Deciphering the Magainin Resistance Process of Escherichia coli Strains in Light of the Cytosolic Proteome

Maria-Neto

Cândido

Rodrigues

et al. 2012

Antimicrob Agents Chemother

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e Antimicrobial peptides (AMPs) are effective antibiotic agents commonly found in plants, animals, and microorganisms, and they have been suggested as the future of antimicrobial chemotherapies. It is vital to understand the molecular details that define the mechanism of action of resistance to AMPs for a rational planning of the next antibiotic generation and also to shed some light on the complex AMP mechanism of action. Here, the antibiotic resistance of Escherichia coli ATCC 8739 to magainin I was evaluated in the cytosolic subproteome. Magainin-resistant strains were selected after 10 subsequent spreads at subinhibitory concentrations of magainin I (37.5 mg · liter ؊1 ), and their cytosolic proteomes were further compared to those of magainin-susceptible strains through two-dimensional electrophoresis analysis. As a result, 41 differentially expressed proteins were detected by in silico analysis and further identified by tandem mass spectrometry de novo sequencing. Functional categorization indicated an intense metabolic response mainly in energy and nitrogen uptake, stress response, amino acid conversion, and cell wall thickness. Indeed, data reported here show that resistance to cationic antimicrobial peptides possesses a greater molecular complexity than previously supposed, resulting in cell commitment to several metabolic pathways.

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Section: Discussionmentioning

confidence: 97%

Deciphering the Magainin Resistance Process of Escherichia coli Strains in Light of the Cytosolic Proteome

Maria-Neto

Cândido

Rodrigues

et al. 2012

Antimicrob Agents Chemother

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“…51,52 In our lab, we detected a class D b-lactamase produced by F. nucleatum strains in isolates from dental patients. 53 …”

Section: B-lactamasesmentioning

confidence: 97%

Bacterial resistance and the dental professionals’ role to halt the problem

Al‐Haroni

2008

Journal of Dentistry

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“…A study focused on a proteome analysis of ampicillin-resistant Fusobacterium nucleatum was reported by Al-Haroni and colleagues (48). The bacteria were resuspended in lysis buffer and mechanically disrupted.…”

Section: Applications Of Proteomic Analysismentioning

confidence: 99%

Matrix-Assisted Laser Desorption Ionization–Time of Flight (MALDI-TOF) Mass Spectrometry for Detection of Antibiotic Resistance Mechanisms: from Research to Routine Diagnosis

2013

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SUMMARYMatrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) has been successfully applied as an identification procedure in clinical microbiology and has been widely used in routine laboratory practice because of its economical and diagnostic benefits. The range of applications of MALDI-TOF MS has been growing constantly, from rapid species identification to labor-intensive proteomic studies of bacterial physiology. The purpose of this review is to summarize the contribution of the studies already performed with MALDI-TOF MS concerning antibiotic resistance and to analyze future perspectives in this field. We believe that current research should continue in four main directions, including the detection of antibiotic modifications by degrading enzymes, the detection of resistance mechanism determinants through proteomic studies of multiresistant bacteria, and the analysis of modifications of target sites, such as ribosomal methylation. The quantification of antibiotics is suggested as a new approach to study influx and efflux in bacterial cells. The results of the presented studies demonstrate that MALDI-TOF MS is a relevant tool for the detection of antibiotic resistance and opens new avenues for both clinical and experimental microbiology.

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Proteomic analysis of ampicillin‐resistant oral Fusobacterium nucleatum

Cited by 34 publications

References 39 publications

Deciphering the Magainin Resistance Process of Escherichia coli Strains in Light of the Cytosolic Proteome

Deciphering the Magainin Resistance Process of Escherichia coli Strains in Light of the Cytosolic Proteome

Bacterial resistance and the dental professionals’ role to halt the problem

Matrix-Assisted Laser Desorption Ionization–Time of Flight (MALDI-TOF) Mass Spectrometry for Detection of Antibiotic Resistance Mechanisms: from Research to Routine Diagnosis

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