Proteomic Analysis of Anti‐inflammatory Effects of a Kampo (Japanese Herbal) Medicine “Shoseiryuto (Xiao‐Qing‐Long‐Tang)” on Airway Inflammation in a Mouse Model

Abstract

Effects of a Kampo (Japanese herbal) medicine “shoseiryuto (SST, xiao-qing-long-tang in Chinese)”, which has been used for the treatment of allergic bronchial asthma clinically, were examined on ovalbumin (OVA)-sensitized allergic airway inflammation model (i.e., bronchial asthma) in a mouse. When SST was orally administered at 0.5 g kg−1 day−1 from day 1 to 6 after OVA inhalation, SST reduced the inflammation in lung tissue, the number of eosinophils and the OVA-specific immunoglobulin E (IgE) antibody titer in bronchoalveolar lavage (BAL) fluids at 7 days after the OVA inhalation. SST also reduced the airway hyperreactivity at 6 days after the OVA inhalation. Proteomic analysis with the agarose two-dimensional electrophoresis showed that the expression of spectrin α2 was reduced in the lung tissue of OVA-sensitized mice and SST recovered the expression. Western blot and immunohistochemical analyses of lung tissue also confirmed this result. When prednisolone was orally administered at 3 mg kg−1 day−1 from day 1 to 6 after OVA inhalation, the inflammation in lung tissue, the number of eosinophils in BAL fluids and airway hyperreactivity were reduced in the OVA-sensitized mice. However, prednisolone did not reduce the OVA-specific IgE antibody titer in BAL fluids and did not recover the expression of spectrin α2 in lung tissue. These results suggest that at least a part of action mechanism of SST against OVA-sensitized allergic airway inflammation in a mouse model is different from that of prednisolone.

Xiao-Qing-Long-Tang attenuates allergic airway inflammation and remodeling in repetitive Dermatogoides pteronyssinus challenged chronic asthmatic mice model

“…Xiao-QingLong-Tang (XQLT) contains eight herbal complexes and has been commonly used for the clinical treatment of bronchial asthma and allergic rhinitis in Asia (Kung et al, 2006;Nagai et al, 2009). Recently, investigators demonstrated that XQLT improved the quality of life and reduced the symptoms of illness in common cold patients (Byun et al, 2011).…”

Identifying Core Herbal Treatments for Children with Asthma: Implication from a Chinese Herbal Medicine Database in Taiwan

“…Xiao-Qing-Long-Tang (XQLT) is the second most commonly used HF for asthmatic children, and it has extensive effects on decreasing Th2-related immune response, anti-inflammatory, reducing secretion, and has potent bronchodilator effect [25, 26, 34–36]. Also, it is effective in controlling allergic rhinitis [37].…”

Antidepressive‐like effect of a Kampo (traditional Japanese) medicine, kososan (Xiang Su San) in a stress‐induced depression‐like mouse model: Proteomic analysis of hypothalamus

“…Two-dimensional electrophoresis was performed according to the procedure given by Oh-Ishi et al [10][11][12][13].…”