Proteomic analysis of aqueous humor from cataract patients with retinitis pigmentosa

Zhang, Bi Ning; Xiao-ming, WU; Dai, Yunhai; Qi, Benxiang; Fan, Cunyi; Huang, Yusen

doi:10.1002/jcp.30031

Cited by 6 publications

(7 citation statements)

References 31 publications

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“…The fibrotic reaction of residual LECs associated with ACO [ 101 ] and extracellular matrix (ECM) disorder might together work on CCS's pathogenesis in RP [ 34 , 68 , 123 , 124 ]. Recently, a new potential target of stimulating LEC proliferation, tenascin‐C (TNC), was found in a proteomic analysis of the aqueous humor between senile cataract and RP patients with cataracts to be a candidate protein underlying capsular shrinkage pathogenesis [ 138 ].…”

Section: Complicationsmentioning

confidence: 99%

A Review of Complicated Cataract in Retinitis Pigmentosa: Pathogenesis and Cataract Surgery

Hong

Sun

et al. 2020

Journal of Ophthalmology

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Retinitis pigmentosa (RP) is a set of inherited retinal degenerative diseases that affect photoreceptor and retinal pigment epithelial cells (RPEs), possibly associated with some ocular complications, including cataract. The complicated cataract formation is most likely the result of RP-related inflammation response, and the most common morphology category is posterior subcapsular cataract (PSC). Despite the absence of curative pharmacologic treatment, phacoemulsification with intraocular lens implantation to deal with opacification in the lens is preferred due to the considerable visual outcomes. However, the incidence of intraocular and postoperative complications is higher in RP patients than those without, including intraoperative phototoxic retinal damage, posterior capsular opacification (PCO), capsular contraction syndrome (CCS), pseudophakic cystoid macular edema (PCME), increased postoperative intraocular pressure (IOP), and intraocular lens (IOL) dislocation. Hence, it needs much attention to surgery progress and close follow-up. In this review, we discuss the current understanding of RP patients with complicated cataracts from morphology to potential pathogenesis to cataract surgical procedure and provide a concise description and the recommended management of related surgery complications to broaden the knowledge and lower the latent risks to yield better clinical outcomes.

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Section: Complicationsmentioning

confidence: 99%

A Review of Complicated Cataract in Retinitis Pigmentosa: Pathogenesis and Cataract Surgery

Hong

Sun

et al. 2020

Journal of Ophthalmology

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“…Aqueous humor mass spectrometry was conducted by Cloud-Seq Biotech (Shanghai, China) with a Q Exactive Orbitrap Mass Spectrometer (Thermo Fisher Scientific). Detailed detection procedure and data analysis were as described in a previous study ( Zhang et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

Sympathetic Nerve-Mediated Fellow Eye Pain During Sequential Cataract Surgery by Regulating Granulocyte Colony Stimulating Factor CSF3

Fan

et al. 2022

Front. Cell. Neurosci.

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Patients were found to experience more pain during their second eye cataract surgery compared with their first eye surgery. This study aimed to explore the inflammatory alterations along time in the fellow eye after the first eye surgery and to reveal the underlying mechanism. Eighty patients with bilateral cataracts were recruited and were divided into four groups based on the time of having the second eye surgery. The second eye aqueous humor samples were collected just before surgery and analyzed by mass spectrometry and PCR array. Cytokine activity was enriched in the aqueous humor of the contralateral eye with granulocyte colony-stimulating factor CSF3 significantly upregulated at both gene and protein levels. Rabbits with or without superior cervical ganglionectomy (SCGx) were subjected to lensectomy to mimic human situations. In both human and rabbit models, the fellow eye CSF3 peaked at 1 week post the first eye surgery. Consistently, more neutrophils were recruited to the contralateral eye aqueous humor. Corneal sensitivity and trigeminal electrophysiology were recorded to imply the pain severity in rats receiving capsulorrhexis with or without SCGx. A more intense pulse was detected in the contralateral trigeminal ganglion after the rat received one eye surgery. SCGx could effectively reduce the fellow corneal sensitivity and trigeminal nerve pain. These alterations were under direct regulation of the sympathetic nerves on the surgical eye side. Our results suggest that CSF3 and sympathetic activity could serve as potential analgesic targets during ocular surgeries.

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“…As AH also mediates immune responses and helps modulate ocular cell proliferation, differentiation, and wound healing, it may contribute to the regulation of CEC properties [18][19][20][21]. Nevertheless, most of the studies that have conducted proteomic analysis of AH have focused on glaucoma or retinal disorders [17,[22][23][24][25][26]. Recently, a number of studies have reported that immune response in AH can increase oxidative stress and cause senescence of CECs, leading to CECD or graft failure [27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Integrated Analysis of Transcriptome and Proteome of the Human Cornea and Aqueous Humor Reveal Novel Biomarkers for Corneal Endothelial Cell Dysfunction

Moon,

Kim,

Jung

et al. 2023

IJMS

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Earlier studies have reported that elevated protein levels in the aqueous humor (AH) are associated with corneal endothelial cell dysfunction (CECD), but the details of the underlying mechanism as well as specific biomarkers for CECD remain elusive. In the present study, we aimed to identify protein markers in AH directly associated with changes to corneal endothelial cells (CECs), as AH can be easily obtained for analysis. We carried out an in-depth proteomic analysis of patient-derived AH as well as transcriptomic analysis of CECs from the same patients with bullous keratopathy (BK) resulting from CECD. We first determined differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) from CECs and AH in CECD, respectively. By combining transcriptomic and proteomic analyses, 13 shared upregulated markers and 22 shared downregulated markers were observed between DEGs and DEPs. Among these 35 candidates from biomarker profiling, three upregulated markers were finally verified via data-independent acquisition (DIA) proteomic analysis using additional individual AH samples, namely metallopeptidase inhibitor 1 (TIMP1), Fc fragment of IgG binding protein (FCGBP), and angiopoietin-related protein 7 (ANGPTL7). Furthermore, we confirmed these AH biomarkers for CECD using individual immunoassay validation. Conclusively, our findings may provide valuable insights into the disease process and identify biofluid markers for the assessment of CEC function during BK development.

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Proteomic analysis of aqueous humor from cataract patients with retinitis pigmentosa

Cited by 6 publications

References 31 publications

A Review of Complicated Cataract in Retinitis Pigmentosa: Pathogenesis and Cataract Surgery

A Review of Complicated Cataract in Retinitis Pigmentosa: Pathogenesis and Cataract Surgery

Sympathetic Nerve-Mediated Fellow Eye Pain During Sequential Cataract Surgery by Regulating Granulocyte Colony Stimulating Factor CSF3

Integrated Analysis of Transcriptome and Proteome of the Human Cornea and Aqueous Humor Reveal Novel Biomarkers for Corneal Endothelial Cell Dysfunction

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