Proteomic Analysis of Atrial Appendages Revealed the Pathophysiological Changes of Atrial Fibrillation

Abstract

Atrial fibrillation (AF), known as the most common arrhythmia in the developed world, affects 1.5-2.0% of the population. Numerous basic studies have been carried out to identify the roles of electric and structural remodeling in the pathophysiological changes of AF, but more explorations are required to further understand the mechanisms of AF development. Proteomics enables researchers to identify protein alterations responsible for the pathological developing progresses of diseases. Compared to the genome, the proteome is closely related to the disease phenotype and can better manifest the progression of diseases. In this study, AF patients proteomically analyzed to identify possible mechanisms. Totally 20 patients undergoing cardiac surgery (10 with paroxysmal AF and 10 with persistent AF) and 10 healthy subjects were recruited. The differentially expressed proteins identified here included AKR1A1,

Blood-Based Biomarkers to Search for Atrial Fibrillation in High-Risk Asymptomatic Individuals and Cryptogenic Stroke Patients

“…On the other hand, DKK3, a member of the dickkopf family, is a cardiac-enriched protein that plays an important role in heart development and presents cardioprotective functions as a negative regulator of pressure overload-induced cardiac hypertrophy (24). In AF, increased DKK-3 levels have been previously reported, in atrial and blood samples (15,25).…”

“…Angiopoietin-2 (Ang-2) is an endothelial growth factor reported to be increased in AF (12)(13)(14). Dickkopf-related protein 3 (DKK-3) is involved in heart development and cardiac hypertrophy protection and, in AF patients, elevated levels have been found in the atrial appendages and in circulation (15,16). Finally, insulin-like growth factorbinding protein 7 (IGFBP-7) is a marker of myocardial damage that has been independently associated with chronic heart failure in AF patients (17).…”

X‐linked ichthyosis: New insights into a multi‐system disorder

“…99 Laminin (including LAMC1) protein levels differ in patients with and without atrial fibrillation. 100,101 LAMC1 gene implicated in arrhythmia. 102 Laminins (including LAMC1) important in regulation of heart looping, atrial growth and cardiac size during development.…”

Microprotein Dysregulation in the Serum of Patients with Atrial Fibrillation

“…Recently, proteomics has obtained successful applications in the study of AF. 15 For example, Ko et al identified 8 proteins associated with the risk of incident AF. 16 Though proteomics provides a promising method for discovering key proteins associated with AF development, the traditional processing method of it will lead to a bias on canonical proteins with large molecule weights.…”

“…Benefiting from high throughput and sensitivity, mass spectrometry (MS)-based proteomics has achieved great progress and became a powerful tool for novel disease indicator discovery. Recently, proteomics has obtained successful applications in the study of AF . For example, Ko et al identified 8 proteins associated with the risk of incident AF .…”