Proteomic Analysis of Biomaterial Surfaces after Contacting with Body Fluids by MALDI-ToF Mass Spectroscopy

Abstract

We developed a method to identify proteins adsorbed on solid surfaces from a solution containing a complex mixture of proteins by using Matrix-Assisted Laser Desorption/Ionization-Time of Flight mass (MALDI-ToF mass) spectroscopy. In the method, we performed all procedures of peptide mass fingerprint method including denaturation, reduction, alkylation, digestion, and spotting of matrix on substrates. The method enabled us to avoid artifacts of pipetting that could induce changes in the composition. We also developed an algorithm to identify the adsorbed proteins. In this work, we demonstrate the identification of proteins adsorbed on self-assembled monolayers (SAMs). Our results show that the composition of proteins on the SAMs critically depends on the terminal groups of the molecules constituting the SAMs, indicating that the competitive adsorption of protein molecules is largely affected by protein-surface interaction. The method introduced here can provide vital information to clarify the mechanism underlying the responses of cells and tissues to biomaterials.

Coatings Functionalization via Laser versus Other Deposition Techniques for Medical Applications: A Comparative Review

“…Laser ablation is a powerful and promising technique [67] for a wide range of applications, from biomedicine (tissue excision) [68], to characterization of biomaterials in analytical chemistry (assisted-matrix laser desorption ionization, MALDI) [69,70] and the structuring of polymers in the field of microelectronics [67], in addition to the preservation of works of art [71].…”

Protein- and Cell-Resistance of Zwitterionic Peptide-Based Self-Assembled Monolayers: Anti-Biofouling Tests and Surface Force Analysis

“…Our results here are in good agreement with previous findings. The analysis of the protein composition by mass spectroscopy is underway (Hirohara et al, 2019).…”

Interplays of Interfacial Forces Modulate Structure and Function of Soft and Biological Matters in Aquatic Environments

“…This has a significant importance in a versatile of applications, ranging from the coating of medical devices such as contact lenses and implants to the protection of ships against the marine biofilms. The exact identification of proteins interacting with the material surface using the proteomic profiling becomes more important for biomedical applications because the body fluid, such as serum, contains various proteins (Hirohara et al, 2019). In recent several decades, a number of protein/cell-repelling materials have been developed, including poly-and oligoethylene glycols (Prime and Whitesides, 1993;Otsuka et al, 2012) and bioinspired dopamine-derivatives (Dalsin et al, 2005;Lee et al, 2007).…”