Proteomic analysis of breast cancer tissue reveals upregulation of actin‐remodeling proteins and its relevance to cancer invasiveness

Abstract

There is an emerging interest in protein expression profiling with the aim of identifying novel diagnostic markers and therapeutic targets in breast cancer. We analyzed breast cancer tissues by 2-D DIGE using a narrow range IPG strip (pH 5.5-6.7) after the immunodepletion of serum albumin and Ig. Sixty-three protein spots were detected with more than 61.8-fold differences (p ,0.05 for three technical replicates) from a set of tissue samples in which three tumor and three nontumor samples were randomly selected from six breast cancer subjects and pooled separately. Of these, 53 proteins were successfully identified by MS. Among the proteins whose levels were increased, we identified three novel WD-repeat-motifbearing proteins that have been known to be involved in actin remodeling: Arp2/3 complex subunit 2 (p34-Arc), coronin-1A and WD-repeat protein 1 (Wdr1). Significantly increased amounts of p34-Arc and coronin-1A in breast cancer were also shown by Western blot analysis of matched tumor and nontumor tissue samples (N = 11, p ,0.05), and were consistent with the mRNA levels retrieved from publicly available microarray databases. The siRNA knockdown of p34-Arc attenuated the invasion of SK-BR3 breast cancer cells into Matrigel. In contrast, the overexpression of coronin-1A increased this invasive activity. Taken together, the cellular levels of p34-Arc and coronin-1A were linked to cancer development and migration. The data obtained from the present study provides new insight into the management of breast cancer.

Quantitative proteomics in resected renal cancer tissue for biomarker discovery and profiling

“…The choice was based on the reports of these two proteins involved in key pathways of kidney or any other cancers in the literature as suggested in the Materials and Methods. Coronin 1A is an actin remodelling protein that has been reported to be overexpressed in breast cancer (Kim et al , 2009a; Klopfleisch et al , 2010). Our immunohistochemical results revealed that Coronin 1A is not expressed by the cells of the primary renal cancer (Figure 3A), but was highly expressed in the infiltrating lymphocytes in all the eight renal cancer specimens.…”

“…Coronin 1A could be a potential biomarker to assess the degree of TILs in the renal cancer, but this needs to be further studied. Coronin 1A was shown to be significantly correlated with development and migration of breast cancer cells (Kim et al , 2009a), compared with normal cell lines. Upregulation of Coronin 1A has been reported by others in RCC samples (Perroud et al , 2009).…”

CRN2 enhances the invasiveness of glioblastoma cells

“…30 Moreover, coronin 1A was found to be elevated in human breast cancer, and over-expression of coronin 1A in SK-BR3 breast cancer cells increased their invasion into Matrigel. 31 …”

Novel TG‐FGFR1 and TRIM33‐NTRK1 transcript fusions in papillary thyroid carcinoma

“…WDR1 plays a crucial role in cytokinesis and cell migration and may be important in the ability of cancer cells to proliferate and invade surrounding tissues . Overexpression of WDR1 was reported in different cancers, including breast cancer, ovarian carcinoma, and thyroid neoplasia …”