Proteomic analysis of cast cuticles from Anopheles gambiae by tandem mass spectrometry

He, Ningjia; Botelho, Julianne M.C.; McNall, Rebecca J.; Belozerov, Vladimir E.; Dunn, William A.; Mize, Todd H.; Orlando, Ron; Willis, Judith H.

doi:10.1016/j.ibmb.2006.10.011

Cited by 88 publications

(121 citation statements)

References 37 publications

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“…Proteomics analyses by MS/MS of various cuticle preparations identified peptides, either unique or shared among similar proteins, for 93% of the 156 CPR proteins, validating their authenticity as cuticular proteins (Cornman et al, 2007;He et al, 2007). The present study was designed to answer the following questions that might help us to understand why An.…”

Section: Introductionmentioning

confidence: 94%

Developmental expression patterns of cuticular protein genes with the R&R Consensus from Anopheles gambiae

Togawa

Dunn

Emmons

et al. 2008

Insect Biochemistry and Molecular Biology

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106

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CPR proteins are the largest cuticular protein family in arthropods. The whole genome sequence of Anopheles gambiae revealed 156 genes that code for proteins with the R&R Consensus and named CPRs. This protein family can be divided into RR-1 and RR-2 subgroups, postulated to contribute to different regions of the cuticle. We determined the temporal expression patterns of these genes throughout post-embryonic development by means of real-time qRT-PCR. Based on expression profiles, these genes were grouped into 21 clusters. Most of the genes were expressed with sharp peaks at single or multiple periods associated with molting. Genes coding for RR-1 and RR-2 proteins were found together in several co-expression clusters. Twenty-five genes were expressed exclusively at one metamorphic stage. Five out of six X-linked genes showed equal expression in males and females, supporting the presence of a gene dosage compensation system in An. gambiae. Many RR-2 genes are organized into sequence clusters whose members are extremely similar to each other and generally closely associated on a chromosome. Most genes in each sequence cluster are expressed with the same temporal expression pattern and at the same level, suggesting a shared mechanism to regulate their expression.

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Section: Introductionmentioning

confidence: 94%

Developmental expression patterns of cuticular protein genes with the R&R Consensus from Anopheles gambiae

Togawa

Dunn

Emmons

et al. 2008

Insect Biochemistry and Molecular Biology

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106

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“…Localization of MpRPS2 in cell membranes would explain its role as a receptor in the transmission process. Interestingly, an equivalent RPS2 product was identified in cast cuticles from Anopheles gambiae after moulting (He et al, 2007), a fact that provides at least preliminary evidence for its presence in insect cuticles. Further work will be needed to confirm whether this is also the case for aphids, and particularly for the structures considered essential for virus transmission that have been described recently in aphid stylets (Uzest et al, 2010).…”

Section: Discussionmentioning

confidence: 89%

The helper-component protease transmission factor of tobacco etch potyvirus binds specifically to an aphid ribosomal protein homologous to the laminin receptor precursor

Fernández‐Calvino

Goytia

López‐Abella

et al. 2010

Journal of General Virology

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Potyviruses are plant pathogens transmitted by aphids in a non-persistent manner. During transmission, the virus-encoded factor helper-component protease (HCPro) is presumed to act as a molecular bridge, mediating the reversible retention of virions to uncharacterized binding sites in the vector mouthparts. Whilst the predicted interaction between HCPro and the coat protein (CP) of virions has been confirmed experimentally, the characterization of putative HCPro-specific receptors in aphids has remained elusive, with the exception of a report that described binding of HCPro of zucchini yellow mosaic virus to several cuticle proteins. To identify other aphid components that could play a role during transmission, this study used purified HCPro of tobacco etch virus (TEV) in far-Western blotting assays as bait to select interactors among proteins extracted from aphid heads. With this approach, new HCPro-interacting proteins were found, and several were identified after mass spectrometry analysis and searches in databases dedicated to aphid sequences. Among these interactors, a ribosomal protein S2 (RPS2) was chosen for further investigation due to its homology with the laminin receptor precursor, known to act as the receptor of several viruses. The specific interaction between RPS2 and TEV HCPro was confirmed after cloning and heterologous expression of the corresponding Myzus persicae gene. The possible involvement of RPS2 in the transmission process was further suggested by testing a variant of HCPro that was non-functional for transmission due to a mutation in the conserved KITC motif (EITC variant). This variant retained its ability to bind CP but failed to interact with RPS2. INTRODUCTIONNatural dissemination of most plant viruses is mediated by insect vectors, in either circulative or non-circulative processes, with aphids (Hemiptera, Aphididae) being the most common vectors (Pirone & Perry, 2002;Ng & Falk, 2006;Hogenhout et al., 2008). Whilst the viral components implicated in transmission are frequently well known, there is substantially less knowledge about the insect counterparts, and only a few published studies have identified insect products with the capacity to interact with virions or viral components that might play a role in plant virus transmission. Using far-Western blotting or similar methodologies, studies with circulative plant viruses have found proteins interacting with virions at key points of the pathways followed by these viruses inside their insect vectors. Examples include a thrips-transmitted tospovirus (Medeiros et al., 2000) and several aphid-transmitted members of the family Luteoviridae (van den Heuvel et al., 1997;Li et al., 2001;Seddas et al., 2004). A promising approach for further advances in this field is represented by the recent adoption of combined genetic and proteomic tools in the analysis of vector specificity in luteovirids (Yang et al., 2008).In the case of non-circulative plant viruses, the insect vector factors implicated in transmission remain largely unknown. The ...

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“…We then had the peptides for LC-MS/MS analyses. We identified numerous CPs, including many CPs that had no relatives in the limited data available from other species (15).…”

Section: Wwwannualreviewsorg • Arthropod Proteomics and Genomicsmentioning

confidence: 99%

The Evolution and Metamorphosis of Arthropod Proteomics and Genomics

Willis

2018

Annu. Rev. Entomol.

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This article presents an overview of the development of techniques for analyzing cuticular proteins (CPs), their transcripts, and their genes over the past 50 years based primarily on experience in the laboratory of J.H. Willis. It emphasizes changes in the kind of data that can be gathered and how such data provided insights into the molecular underpinnings of insect metamorphosis and cuticle structure. It describes the techniques that allowed visualization of the location of CPs at both the anatomical and intracuticular levels and measurement of the appearance and deployment of transcripts from CP genes as well as what was learned from genomic and transcriptomic data. Most of the early work was done with the cecropia silkmoth, Hyalophora cecropia, and later work was with Anopheles gambiae.

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Proteomic analysis of cast cuticles from Anopheles gambiae by tandem mass spectrometry

Cited by 88 publications

References 37 publications

Developmental expression patterns of cuticular protein genes with the R&R Consensus from Anopheles gambiae

Developmental expression patterns of cuticular protein genes with the R&R Consensus from Anopheles gambiae

The helper-component protease transmission factor of tobacco etch potyvirus binds specifically to an aphid ribosomal protein homologous to the laminin receptor precursor

The Evolution and Metamorphosis of Arthropod Proteomics and Genomics

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