Proteomic analysis of changes in protein expression in serum from animals exposed to paraquat

Abstract

Abstract. Paraquat (PQ) poisoning remains a major public health concern in many countries. Extensive research has focused on finding early diagnostic biomarkers of acute PQ poisoning. In order to investigate the characterization of diagnostic biomarkers in PQ poisoning, we utilized proteomic analysis using serum from rats exposed to PQ, and we identified 8 differentially expressed proteins from over 500 protein spots. The expression of apolipoprotein E (ApoE), preprohaptoglobin (Pphg), a precursor of haptoglobin (Hp), and complement component 3 (C3) proteins was greatly induced by PQ exposure while the expression of fibrinogen γ-chain (FGG) and Ac-158 was dramatically reduced. To further investigate the possibility of ApoE, Pphg and FGG as useful diagnostic biomarkers of PQ poisoning, western blot and qRT-PCR analyses were conducted using cell lines as well as rat and human sera. The expression levels of ApoE, Hp and FGG were significantly altered in the presence of PQ in both rat and human serum suggesting that these proteins may be appropriate candidate molecular biomarkers for the early diagnosis of acute PQ intoxication.

Pulmonary Proteome and Protein Networks in Response to the Herbicide Paraquat in Rats

“…However, our work presented that NADH dehydrogenase (ubiquinone) iron-sulfur protein 8, which is related to electron transport, was found to be down-regulated in the treatment sample ( Supplemental data Table 4 ). It is noteworthy that Kim et al [ 24 ] reported great induction of ApoE, Pphg, Hp and C3 proteins and dramatic reduction of FGG and Ac-158 in sera upon PQ exposure, which, however, were not detected in the lung tissues in the present study ( Supplementary data Table 5 ).…”

“…Early diagnostic biomarkers are important for treatment of acute PQ poisoning. In order to investigate the characterization of diagnostic biomarkers in PQ poisoning, Kim et al [ 24 ] employed a 2D-PAGE MALDI-TOF/TOF approach and identified that apolipoprotein E (ApoE), a precursor of haptoglobin (Hp) and fibrinogen γ-chain (FGG) were potential biomarkers of PQ exposure in rat sera. In an effort to develop a remedy for PQ poisoning, Kim et al [ 25 ] conducted proteomic analysis of PQ-poisoned male CD(SD)IGS rat lungs to identify any protective effects of acai berry on the PQ poisoning.…”

Pesticide Use and Age-Related Macular Degeneration in the Agricultural Health Study

“…These persistent lipophilic compounds could accumulate in the retina and increase oxidative stress in response to light exposure. Potentially of great importance to AMD are observations that complement pathways can be activated by some pesticides, including DDT ( Dutta et al 2008 ), other organochlorine insecticides ( Kumar et al 2014 ), malathion ( Ayub et al 2001 ), the pyrethroid fenvalerate ( Dutta and Das 2011 ), and paraquat ( Kim YS et al 2012 ).…”