Proteomic analysis of cucumber seedling roots subjected to salt stress

Proteomic response of wheat embryos to fosthiazate stress in a protected vegetable soil

“…Another protein, cytosolic aconitase (spot 32) related to the citrate cycle, decreased significantly compared to the control. This was similar to Du et al (2010), who found that enzymes involved in the citrate cycle (pyruvate dehydrogenase E1, malate dehydrogenase) were down-regulated in cucumber subjected to salt stress, suggesting carbohydrate metabolism and energy consumption reduction. Thus, glycolysis may be the main energy supply in germinating wheat seeds under fosthiazate stress.…”

“…Metabolism-related proteins were found to be significantly up-or down-regulated during germination in wheat, as detected in tomato, rice and cucumber (Ahsan et al, 2007b(Ahsan et al, , 2007cDu et al, 2010). In the present study we detected differential expression of proteins involved in carbohydrate, fatty acid and amino acid metabolism.…”

“…As suggested by Du et al (2010), up-regulation of vacuolar proton-ATPase subunit A was associated with osmotic adjustment. In our study the expression of this enzyme was more than halved compared to the untreated embryos, indicating that fosthiazate might cause osmotic stress to wheat at the germination stage.…”

“…Up-regulation of FBP aldolase during germination might indicate that seeds need more energy to cope with fosthiazate stress. However, FBP aldolase was observed to decrease under salt stress in cucumber and flooding stress in wheat (Du et al, 2010;Kong et al, 2010). Another protein, cytosolic aconitase (spot 32) related to the citrate cycle, decreased significantly compared to the control.…”

“…2-DE is used to investigate differentially-expressed proteins in response to abiotic or biotic stresses such as heavy metals (Ahsan et al, 2007a(Ahsan et al, , 2007c, herbicides (Ahsan et al, 2008;Castro et al, 2005;Lin et al, 2008), flooding (Ahsan et al, 2007b;Kong et al, 2010), salt stress (Du et al, 2010), chilling , ovine saliva , Meloidogyne incognita (de Deus Barbosa, 2009) and pathogens (Lee, 2006). It is well-documented that the germination process is highly disturbed by heavy metals (Xu et al, 2009), herbicides (Song et al, 2007) and antibiotics (Jin et al, 2010).…”