Proteomic analysis of drought-responsive proteins in rice reveals photosynthesis-related adaptations to drought stress

Chintakovid, Nutwadee; Maipoka, Maiporn; Phaonakrop, Narumon; Mickelbart, Michael V.; Roytrakul, Sittiruk; Chadchawan, Supachitra

doi:10.1007/s11738-017-2532-4

Cited by 34 publications

(16 citation statements)

References 102 publications

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“…Comparative proteomic analysis is a powerful tool for the study of plant stress response [31,46]. The changes in protein expression profiles under drought conditions have been investigated in several plants, including wheat, maize, rice, peanut, and soybean, and many drought-responsive proteins have been characterized [32,33,34,35,36,37]. However, despite this progress, most of these proteins have not been functionally verified.…”

Section: Discussionmentioning

confidence: 99%

“…In most cases, proteins are the ultimate functional molecules; therefore, proteomics has become a powerful and promising tool for the study of plant stress response [31]. However, although many drought-responsive proteins have been characterized [32,33,34,35,36,37], most of them have not been functionally verified. One important reason is that functional verification by transgenic research in some polyploid crops, especially in hexaploid wheat, is time-consuming and not suitable for high-throughput studies.…”

Section: Introductionmentioning

confidence: 99%

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Identification of Two Novel Wheat Drought Tolerance-Related Proteins by Comparative Proteomic Analysis Combined with Virus-Induced Gene Silencing

Wang

Yan

et al. 2018

IJMS

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Drought is a major adversity that limits crop yields. Further exploration of wheat drought tolerance-related genes is critical for the genetic improvement of drought tolerance in this crop. Here, comparative proteomic analysis of two wheat varieties, XN979 and LA379, with contrasting drought tolerance was conducted to screen for drought tolerance-related proteins/genes. Virus-induced gene silencing (VIGS) technology was used to verify the functions of candidate proteins. A total of 335 differentially abundant proteins (DAPs) were exclusively identified in the drought-tolerant variety XN979. Most DAPs were mainly involved in photosynthesis, carbon fixation, glyoxylate and dicarboxylate metabolism, and several other pathways. Two DAPs (W5DYH0 and W5ERN8), dubbed TaDrSR1 and TaDrSR2, respectively, were selected for further functional analysis using VIGS. The relative electrolyte leakage rate and malonaldehyde content increased significantly, while the relative water content and proline content significantly decreased in the TaDrSR1- and TaDrSR2-knock-down plants compared to that in non-knocked-down plants under drought stress conditions. TaDrSR1- and TaDrSR2-knock-down plants exhibited more severe drooping and wilting phenotypes than non-knocked-down plants under drought stress conditions, suggesting that the former were more sensitive to drought stress. These results indicate that TaDrSR1 and TaDrSR2 potentially play vital roles in conferring drought tolerance in common wheat.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of Two Novel Wheat Drought Tolerance-Related Proteins by Comparative Proteomic Analysis Combined with Virus-Induced Gene Silencing

Wang

Yan

et al. 2018

IJMS

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“…Twenty-eight days after germination, rice plants were drought-stressed for three days by the addition of 10% polyethylene glycol 6000 (PEG6000). This condition was previously shown to cause drought stress in rice [ 14 , 15 ]. In order to induce the stronger drought-stress condition, after treatment with WP nutrient solution with 10% PEG6000 for three days, the solution was then changed to WP nutrient solution with 15% PEG.…”

Section: Methodsmentioning

confidence: 99%

Drought-Tolerance Gene Identification Using Genome Comparison and Co-Expression Network Analysis of Chromosome Substitution Lines in Rice

Punchkhon

Plaimas

Buaboocha

et al. 2020

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Drought stress limits plant growth and productivity. It triggers many responses by inducing changes in plant morphology and physiology. KDML105 rice is a key rice variety in Thailand and is normally grown in the northeastern part of the country. The chromosome segment substitution lines (CSSLs) were developed by transferring putative drought tolerance loci (QTLs) on chromosome 1, 3, 4, 8, or 9 into the KDML105 rice genome. CSSL104 is a drought-tolerant line with higher net photosynthesis and leaf water potential than KDML105 rice. The analysis of CSSL104 gene regulation identified the loci associated with these traits via gene co-expression network analysis. Most of the predicted genes are involved in the photosynthesis process. These genes are also conserved in Arabidopsis thaliana. Seven genes encoding chloroplast proteins were selected for further analysis through characterization of Arabidopsis tagged mutants. The response of these mutants to drought stress was analyzed daily for seven days after treatment by scoring green tissue areas via the PlantScreen™ XYZ system. Mutation of these genes affected green areas of the plant and stability index under drought stress, suggesting their involvement in drought tolerance.

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“…In Brassica and wheat, the Chl a/b ratio was highly reduced in susceptible cultivars under drought stress, but was slightly increased in tolerant genotypes (Ashraf and Mehmood, 1990 ; Ashraf, 1994 ). In rice, helicase domain-containing proteins have shown up-regulation in response to drought stress and functions as maintaining photosynthesis and antioxidant machinery (Ambavaram et al, 2014 ; Chintakovid et al, 2017 ), Moreover, the genes encoding GAPDH and FNR [the key enzymes influencing NADP(H) homeostasis are affected by osmotic-stress treatments], suggests that drought tolerance in rice may be mediated by photosynthesis-related adaptations by utilizing the NADP(H) homeostasis.…”

Section: Major Factors Limiting Photosynthesis and Plant Growthmentioning

confidence: 99%

“…Another enzyme, ferredoxin-NADP reductase (FNR), helps to channelize electronic transport and redox homeostasis within chloroplasts (Chinthapalli et al, 2003 ). Different abiotic stresses affect the activity of FNR differently; for example, in transgenic tobacco plants, FNR level was reduced by drought stress (Gharechahi et al, 2015 ), whereas in Paeonia cathayana (Xiao et al, 2009 ), wheat (Budak et al, 2013 ), rice (Nouri et al, 2015 ; Chintakovid et al, 2017 ), and maize, FNR levels was increased by salt stress (Zörb et al, 2009 ). In rice, GAPDH activity was up-regulated, whereas FNR level was reduced under osmotic stress (Chintakovid et al, 2017 ).…”

Section: Effects On Activities Of Key Photosynthetic Enzymesmentioning

confidence: 99%

Mechanisms Regulating the Dynamics of Photosynthesis Under Abiotic Stresses

et al. 2021

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Photosynthesis sustains plant life on earth and is indispensable for plant growth and development. Factors such as unfavorable environmental conditions, stress regulatory networks, and plant biochemical processes limits the photosynthetic efficiency of plants and thereby threaten food security worldwide. Although numerous physiological approaches have been used to assess the performance of key photosynthetic components and their stress responses, though, these approaches are not extensive enough and do not favor strategic improvement of photosynthesis under abiotic stresses. The decline in photosynthetic capacity of plants due to these stresses is directly associated with reduction in yield. Therefore, a detailed information of the plant responses and better understanding of the photosynthetic machinery could help in developing new crop plants with higher yield even under stressed environments. Interestingly, cracking of signaling and metabolic pathways, identification of some key regulatory elements, characterization of potential genes, and phytohormone responses to abiotic factors have advanced our knowledge related to photosynthesis. However, our understanding of dynamic modulation of photosynthesis under dramatically fluctuating natural environments remains limited. Here, we provide a detailed overview of the research conducted on photosynthesis to date, and highlight the abiotic stress factors (heat, salinity, drought, high light, and heavy metal) that limit the performance of the photosynthetic machinery. Further, we reviewed the role of transcription factor genes and various enzymes involved in the process of photosynthesis under abiotic stresses. Finally, we discussed the recent progress in the field of biodegradable compounds, such as chitosan and humic acid, and the effect of melatonin (bio-stimulant) on photosynthetic activity. Based on our gathered researched data set, the logical concept of photosynthetic regulation under abiotic stresses along with improvement strategies will expand and surely accelerate the development of stress tolerance mechanisms, wider adaptability, higher survival rate, and yield potential of plant species.

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Proteomic analysis of drought-responsive proteins in rice reveals photosynthesis-related adaptations to drought stress

Cited by 34 publications

References 102 publications

Identification of Two Novel Wheat Drought Tolerance-Related Proteins by Comparative Proteomic Analysis Combined with Virus-Induced Gene Silencing

Identification of Two Novel Wheat Drought Tolerance-Related Proteins by Comparative Proteomic Analysis Combined with Virus-Induced Gene Silencing

Drought-Tolerance Gene Identification Using Genome Comparison and Co-Expression Network Analysis of Chromosome Substitution Lines in Rice

Mechanisms Regulating the Dynamics of Photosynthesis Under Abiotic Stresses

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