Proteomic analysis of early phase of conidia germination in Aspergillus nidulans

Photodynamic treatment with phenothiazinium photosensitizers kills both ungerminated and germinated microconidia of the pathogenic fungi Fusarium oxysporum, Fusarium moniliforme and Fusarium solani

“…Activation is followed by isotropic growth which is the first morphological change that can be observed during germination and involves water uptake and cell wall growth. It occurs simultaneously with the resumption of numerous metabolic activities including respiration, and RNA and protein synthesis and results in a cell whose diameter is two to several times that of the resting conidia [15,16,18]. The last stage is the polarized growth which ends with the formation of a germ tube [13].…”

“…In addition to the marked difference in metabolic activity, ungerminated conidia differ from germlings in their biochemical composition, redox status, cell organization and structure and composition of the cell wall [15][16][17][18][19]. As conidium and germling are essential developmental phases in the fungal life cycle and critical for plant colonization, most conventional fungicides have been developed to target the conidial germination and early developmental stages [19].…”

A Review beyond the borders: Proteomics of microclonial black fungi and black yeasts

“…It is recommendable to try different concentrations of CHAPS for the most desirable protein yield, which is to be optimized case to case [14]. DTT and ß-mercaptoethal were used as reducing agents in the homogenization buffer [24,25]. They reduce the disulfide bonds and enhance the protein solubility.…”

Proteomics Methodology Applied to the Analysis of Filamentous Fungi - New Trends for an Impressive Diverse Group of Organisms

“…Thus, the use of liquid nitrogen to decrease warming of the disruption systems is a widely used method. The main cell-breaking system is the traditional pre-chilled mortar grinding due to its efficiency against the fungal cell wall (Cobos et al, 2010;Lu et al, 2010;Vödisch et al, 2011), although waring blender machines (Lim et al, 2001), or glass bead beating systems, either combined with a 10 mM Tris-HCl buffer (Oh et al, 2010) or with a phenol buffer (Coumans et al, 2010;Vodisch et al, 2011), have been successfully applied. After the breaking step, the protein solubilisation buffer always includes a protease inhibitor [e.g.…”

“…Under long-term oxidative stress, the peroxide-detoxifying peroxiredoxins and cytochrome C peroxidase were replaced by thioredoxin reductase, a nitroreductase and a flavohemoprotein, and protein degradation became predominant to eliminate damaged proteins (Pusztahelyi et al, 2011). Those proteins involved in early phase of conidia germination were also analysed through Proteomics using 2D in conjunction with a Voyger-DE STR MALDI-TOF (PerSeptive Biosystems) mass spectrometer (Oh et al, 2010). During the early phase of the germination stage, levels of proteins involved in metabolism, protein synthesis and transcription highly increased, confirming the importance of metabolic activation and new protein synthesis for the germination process.…”