Proteomic analysis of endothelial cell secretome: A means of studying the pleiotropic effects of Hmg-CoA reductase inhibitors

Brioschi, Maura; Lento, Sabrina; Tremoli, Elena; Banfi, Cristina

doi:10.1016/j.jprot.2012.10.003

Cited by 38 publications

(49 citation statements)

References 68 publications

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“…Studies have shown that reactive oxygen species, such as the superoxide anion (·O 2-), hydroxyl radical (·OH), and hydrogen peroxide (H 2 O 2 ), are the major factors that bind as ligands to nuclear receptors in human umbilical vein endothelial cells (HUVECs), participate and regulate the expression of several genes, enhance mononuclear cell adhesion and immigration to the vascular intima, and play a pivotal role in the initial stage of AS, diabetes, hypertension, and heart failure (Yanamoto et al, 2012;Brioschi et al, 2013;Choi et al, 2013;Montoro-García et al, 2013). …”

Section: Introductionmentioning

confidence: 99%

Protective effect of ultrafiltered XinMaiJia extract against H2O2-induced injury in human umbilical vein endothelial cells through NHE1 downregulation

Yin¹,

Li²,

Yang³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. We examined the protective effects of ultrafiltered XinMaiJia (XMJ) extract in a hydrogen peroxide (H 2 O 2 )-induced injury model in human umbilical vein endothelial cells (HUVECs) and determined the corresponding changes in the Na indicators, cytokine concentration, NHE1 protein content, and NHE1 gene expression were determined. H 2 O 2 significantly promoted HUVEC injury, whereas ultrafiltered XMJ extract significantly improved the morphological changes in injured HUVECs, increased their activity, and decreased NHE1 gene and protein expression, as well as limited the decrease in membrane permeability and expression of intercellular adhesion molecule-1, vascular cell adhesion molecule-1, interleukin (IL)-1, IL-6, and nuclear factor-kB. Ultrafiltered XMJ extract inhibited H 2 O 2 -induced HUVEC injury by inhibiting NHE1 activity.

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Section: Introductionmentioning

confidence: 99%

Protective effect of ultrafiltered XinMaiJia extract against H2O2-induced injury in human umbilical vein endothelial cells through NHE1 downregulation

Yin¹,

Li²,

Yang³

et al. 2014

Genet. Mol. Res.

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“…The cell pellets were dissolved in 25 mmol/L NH 4 HCO 3 containing 0.1% RapiGest (Waters 226 Corporation, Milford, MA, USA) and digested as previously described [23]. The tryptic peptides 227…”

Section: Label-free Lc-ms E Analysis 225mentioning

confidence: 99%

“…Ion detection, data clustering, and the normalisation of 234 the data-independent LC-MS E data have been previously explained in detail [23,25,26]. 235…”

Section: Label-free Lc-ms E Analysis 225mentioning

confidence: 99%

“…between 0 and 0.05), the likelihood of down-regulation is >95%; if it is >0.95 242 (i.e., between 0.95 and 1) the likelihood of up-regulation is >95% [23]. 243 The significance of the regulation level was determined at a 20% fold change, but only proteins 244 identified with at least 2 peptides with similar intensities in the technical replicates (CV<10%) 245…”

Section: Label-free Lc-ms E Analysis 225mentioning

confidence: 99%

“…Cytoscape (v 2.7) and BiNGO plugin (v 2.3) were used to make gene ontology (GO) assignments 265 and determine the cell component and biological process GO categories that were significantly 266 under-and over-represented, as previously described [23]. Statistical significance was determined 267…”

Section: Data Mining 264mentioning

confidence: 99%

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Proteomics of tissue factor silencing in cardiomyocytic cells reveals a new role for this coagulation factor in splicing machinery control

Lento

Brioschi

Barcella

et al. 2015

Journal of Proteomics

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22It has long been known that Tissue Factor (TF) plays a role in blood coagulation and has a direct 23 thrombotic action that is closely related to cardiovascular risk, but it is becoming increasingly 24clear that it has a much wider range of biological functions that range from inflammation to 25 immunity. It is also involved in maintaining heart hemostasis and structure, and the observation 26 that it is down-regulated in the myocardium of patients with dilated cardiomyopathy suggests that 27 it influences cell-to-cell contact stability and contractility, and thus contributes to cardiac 28 dysfunction. However, the molecular mechanisms underlying these coagulation-independent 29 functions have not yet been fully elucidated. 30In order to analyse the influence of TF on the cardiomyocitic proteome, we used functional 31 biochemical approaches incorporating label-free quantitative proteomics and gene silencing, and 32found that this provided a powerful means of identifying a new role for TF in regulating splicing 33 machinery together with the expression of several proteins of the spliceosome, and mRNA 34 metabolism with a considerable impact on cell viability. 35 36 SIGNIFICANCE 37

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Normal human mitral valve proteome: A preliminary investigation by gel‐based and gel‐free proteomic approaches

et al. 2016

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The mitral valve is a highly complex structure which regulates blood flow from the left atrium to the left ventricle (LV) avoiding a significant forward gradient during diastole or regurgitation during systole. The integrity of the mitral valve is also essential for the maintenance of normal LV size, geometry, and function. Significant advances in the comprehension of the biological, functional, and mechanical behavior of the mitral valve have recently been made. However, current knowledge of protein components in the normal human mitral valve is still limited and complicated by the low cellularity of this tissue and the presence of high abundant proteins from the extracellular matrix. We employed here an integrated proteomic approach to analyse the protein composition of the normal human mitral valve and reported confident identification of 422 proteins, some of which have not been previously described in this tissue. In particular, we described the ability of pre-MS separation technique based on liquid-phase IEF and SDS-PAGE to identify the largest number of proteins. We also demonstrated that some of these proteins, e.g. αB-Crystallin, septin-11, four-and-a-half LIM domains protein 1, and dermatopontin, are synthesised by interstitial cells isolated from human mitral valves. These initial results provide a valuable basis for future studies aimed at analysing in depth the mitral valve protein composition and at investigating potential pathogenetic molecular mechanisms. Data are available via ProteomeXchange with identifier PXD004397.

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Proteomic analysis of endothelial cell secretome: A means of studying the pleiotropic effects of Hmg-CoA reductase inhibitors

Cited by 38 publications

References 68 publications

Protective effect of ultrafiltered XinMaiJia extract against H2O2-induced injury in human umbilical vein endothelial cells through NHE1 downregulation

Protective effect of ultrafiltered XinMaiJia extract against H2O2-induced injury in human umbilical vein endothelial cells through NHE1 downregulation

Proteomics of tissue factor silencing in cardiomyocytic cells reveals a new role for this coagulation factor in splicing machinery control

Normal human mitral valve proteome: A preliminary investigation by gel‐based and gel‐free proteomic approaches

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