Proteomic analysis of gastric cancer and immunoblot validation of potential biomarkers

Kočevar, Nina

doi:10.3748/wjg.v18.i11.1216

Cited by 20 publications

(18 citation statements)

References 98 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TALDO catalyzes the reversible transfer of a three-carbon unit between various sugar phosphates (from ketose to aldose sugar phosphates) (10). A previous study has revealed that TALDO is significantly overexpressed in gastric adenocarcinoma (41). Furthermore, its expression is associated with metastatic behavior in HCC (42).…”

Section: Roles Of Ribose-5-phosphate Isomerase (Rpi) and Ribulose-5-pmentioning

confidence: 99%

Crucial role of the pentose phosphate pathway in malignant tumors (Review)

2019

View full text Add to dashboard Cite

Interest in cancer metabolism has increased in recent years. The pentose phosphate pathway (PPP) is a major glucose catabolism pathway that directs glucose flux to its oxidative branch and leads to the production of a reduced form of nicotinamide adenine dinucleotide phosphate and nucleic acid. The PPP serves a vital role in regulating cancer cell growth and involves many enzymes. The aim of the present review was to describe the recent discoveries associated with the deregulatory mechanisms of the PPP and glycolysis in malignant tumors, particularly in hepatocellular carcinoma, breast and lung cancer. Contents 1. Introduction 2. Glucose in the PPP 3. Glucose breakdown through glycolysis in the PPP 4. PPP in malignant tumors 5. Perspectives

show abstract

Section: Roles Of Ribose-5-phosphate Isomerase (Rpi) and Ribulose-5-pmentioning

confidence: 99%

Crucial role of the pentose phosphate pathway in malignant tumors (Review)

2019

View full text Add to dashboard Cite

show abstract

“…We first separated the proteins in an alkaline pH using the conventional procedures that were used in our previous study (in‐gel rehydration, conventional rehydration solution, DTT as reducing agent; see Supporting Information Fig. 1A).…”

Section: Resultsmentioning

confidence: 99%

“…In the present study, we report on a detailed investigation of gastric tissue samples using 2DE under alkaline conditions. To complement our previous data from the analysis in the acidic pH range , the same samples and detection techniques were used. As 2DE specifically designed for alkaline pHs has not been reported previously for gastric cancer, we first optimized the procedures for this specific purpose.…”

Section: Introductionmentioning

confidence: 99%

Two‐dimensional gel electrophoresis of gastric tissue in an alkaline pH range

2014

Self Cite

View full text Add to dashboard Cite

2DE in combination with MS has facilitated the discovery of several proteins with altered abundance in gastric cancer. While acidic and wide pH ranges have been widely investigated, analysis in the alkaline pH range has not been specifically performed in gastric cancer to date. In the present study, we initially optimized the 2DE in alkaline pH range (pH 7-11) for gastric tissue samples. Using a modified lysis buffer, we analyzed pooled nontumor and tumor samples for proteins with altered abundance in gastric adenocarcinoma. We successfully identified 38 silver-stained spots as 24 different proteins. Four of these were chosen for investigation with immunoblotting on individual paired samples to determine whether the changes seen in 2DE represent the overall abundance of the protein or possibly only a single form. While mitochondrial trifunctional protein (MTP) subunits were decreased in 2DE gels, immunoblotting identified their overall abundance as being differently dysregulated: in the gastric tumor samples, the MTP-α subunit was decreased, and the MTP-β subunit was increased. On the other hand, heterogenous nuclear ribonucleoprotein M and galectin-4 were increased in the gastric tumor samples in both 2DE and immunoblotting.

show abstract

“…Elevated tumour markers are known to be associated with the higher probability of lymph node metastases, lymphatic and blood vessel invasion, depth of invasion, higher stage and dissemination of the disease. Kočevar et al 57 reported that translationally controlled tumour protein (TPT1) was shown to be differentially expressed only in patients GEJ cancer, but, it clinical have to be established.…”

Section: Discussionmentioning

confidence: 99%

Results of postoperative radiochemotherapy of the patients with resectable gastroesophageal junction adenocarcinoma in Slovenia

Jeromen¹,

Oblak²,

Anderluh³

et al. 2012

Radiology and Oncology

View full text Add to dashboard Cite

Background.Although the incidence of adenocarcinomas of the gastroesophageal junction (GEJ) is sharply rising in the Western world, there are still some disagreements about the staging and the treatment of this disease. The aim of this retrospective study was to analyse the effectiveness and safety of postoperative radiochemotherapy in patients with a GEJ adenocarcinoma treated at the Institute of Oncology Ljubljana.Patients and methods.Seventy patients with GEJ adenocarcinoma, who were treated with postoperative radiochemotherapy between January 2005 and June 2010, were included in the study. The treatment consisted of 6 cycles of chemotherapy with 5-FU and cisplatin and concomitant radiotherapy with the total dose of 45 Gy.Results.Twenty-six patients (37.1%) completed the treatment according to the protocol. The median follow-up time was 17.7 months (range: 3.3–64 months). Acute toxicity grade 3 or more, such as stomatitis, dysphagia, nausea or vomiting, and infection, occurred in 2.9%, 34.3%, 38.6% and 41.5% of patients, respectively. At 3 years locoregional control (LRC), disease-free survival (DFS), disease-specific survival (DSS) and overall survival (OS) were 78.2%, 25.3%, 35.8%, and 33.9%, respectively. In the multivariate analysis of survival, splenectomy and level of Ca 19-9 >20 kU/L before the adjuvant treatment were identified as independent prognostic factors for lower DFS, DSS and OS. Age <60 years, higher number of involved lymph nodes and advanced disease stage were identified as independent prognostic factors for lower DSS and OS.Conclusions.In patients with GEJ adenocarcinoma who first underwent surgery, postoperative radiochemotherapy is feasible, but we must be aware of a high risk of acute toxic side effects.

show abstract

Proteomic analysis of gastric cancer and immunoblot validation of potential biomarkers

Abstract: This work presents a set of possible diagnostic biomarkers, validated for the first time. It might contribute to the efforts of understanding gastric cancer carcinogenesis.

Cited by 20 publications

References 98 publications

Crucial role of the pentose phosphate pathway in malignant tumors (Review)

Crucial role of the pentose phosphate pathway in malignant tumors (Review)

Two‐dimensional gel electrophoresis of gastric tissue in an alkaline pH range

Results of postoperative radiochemotherapy of the patients with resectable gastroesophageal junction adenocarcinoma in Slovenia

Contact Info

Product

Resources

About