Proteomic analysis of Giardia: Studies from the pre- and post-genomic era

Abstract

The proteome of Giardia duodenalis has been under study for the last 25 years and has lead to the discovery of valuable information on the biology and variation of the parasite.Proteomic techniques, mainly SDS-PAGE and 2D-PAGE, have been used to investigate protein variation, cellular structure and host parasite interactions. This has allowed for the identification of assemblage and host specific proteins, structural proteins, proteins released by trophozoites upon exposure to host cell monolayers and immunoreactive proteins. These data are important in understanding the pathogenesis of G. duodenalis infections, as well as highlighting potential drug and vaccine targets. There is however a large amount of future work needed to fully understand the proteome of this parasite.

Proteomic analysis inGiardia duodenalisyields insights into strain virulence and antigenic variation

“…Proteomic data already exist in integrated databases for other protozoan parasites of humans , including Plasmodium falciparum , Toxoplasma gondii , and Cryptosporidium parvum . However, published proteomic studies in Giardia are not as comprehensive . There have been several MS‐based studies recently published on the ventral disk, mitosome, and basal body proteins , but there are few published shotgun proteomics experiments aimed at exploring the entire G. duodenalis proteome .…”

Proteomics and Host—Pathogen Interactions

“…By contrast, this represents one major limitation to characterize hostÀpathogen interactions, but also a challenging perspective for proteomics technology. This is why retrospectively proteomics studies were mainly conducted to evidence pathogenic virulence and pathogenicity factors (Ouellette et al, 2003;Texier et al, 2005;Sims and Hyde, 2006;Van Hellemond et al, 2007;Liu et al, 2008a,b;Bird and Opperman, 2009;Jagusztyn-Krynicka et al, 2009;Premsler et al, 2009;Weiss et al, 2009;Steuart, 2010;Bhavsar et al, 2010;Holzmuller et al, 2010).…”