Proteomic Analysis of Humoral Immune Components in Bronchoalveolar Lavage of Patients Infected or Colonized by Aspergillus fumigatus

Dellière, Sarah; Duchateau, Magalie; Wong, Sarah Sze Wah; Gianetto, Quentin Giai; Guegan, Hélène; Matondo, Mariette; Gangneux, Jean‐Pierre; Aimanianda, Vishukumar

doi:10.3389/fimmu.2021.677798

Cited by 7 publications

(11 citation statements)

References 23 publications

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“…An opportunistic pathogen like A. fumigatus can establish infection when the host defense system is suppressed or compromised. Our recent study comparing the proteomics of bronchoalveolar lavage fluid (BALF) from control and A. fumigatus -infected and colonized hosts indicated that SP-D was one of the key humoral immune components depleted in the infected and colonized BALF ( Delliere et al, 2021 ). One of the reasons for this depletion could be due to the degradation of SP-D. Pseudomonas aeruginosa , a Gram-negative bacteria, was shown to secrete an elastase that can degrade SP-D, abrogating its immunological functions ( Alcorn and Wright, 2004 ).…”

Section: Discussionmentioning

confidence: 99%

Surfactant protein D inhibits growth, alters cell surface polysaccharide exposure and immune activation potential of Aspergillus fumigatus

et al. 2022

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Highlights Surfactant protein D (SP-D), a C -type lectin and a major humoral immune component in the human lung-alveoli, shows direct growth inhibitory effect on Aspergillus fumigatus , an airborne opportunistic fungal pathogen. SP-D treatment modifies the surface architecture and cell wall polysaccharide exposure on the A. fumigatus hyphae. A. fumigatus hyphae grown in presence of SP-D stimulate significantly lower secretions of pro-inflammatory cytokine, but higher anti-inflammatory cytokine and chemokine secretions upon interaction with immune cells, compared to hyphae grown without SP-D. Increased permeability of A. fumigatus hyphae upon SP-D treatment leads to a better efficacy of voriconazole, an antifungal drug that has its target in the fungal cytosol.

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Section: Discussionmentioning

confidence: 99%

Surfactant protein D inhibits growth, alters cell surface polysaccharide exposure and immune activation potential of Aspergillus fumigatus

et al. 2022

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“…Amplifies complement cascade. Increases phagocytosis and modulates immune response [ 15 , 20 , 21 ] C5a Complement system NA NA Recruits neutrophils at the site of infection [ 24 , 25 ] Cathelicidin (LL-37) AMP NA ↑ in BAL Discrepant results in the literature [ 41 , 56 , 64 , 65 ] CRP APP NA ↓ in BAL Promotes conidia phagocytosis by neutrophils [ 41 , 72 ] Defensin (α-) AMP NA NA Fungicidal effect on swollen conidia and hyphae [ 58 , 61 ] Defensin (ß-) AMP NA NA Inhibition of germination [ 56 ] Ficolin-1 Collectin Chitin β-1,3-glucan ↑ in BAL Opsonizes hyphae increase IL-8 secretion by lung epithelial cells [ 40 , 41 ] Ficolin-2 Collectin GlcNAc GalNAc ↓ or ↑ in BAL Opsonizes conidia and decrease pro-inflammatory cy...…”

Section: Introductionmentioning

confidence: 99%

“…Inhibition of hyphae metabolism [ 56 ] Lactoferrin AMP NA ↑ in BAL Iron depletion. Inhibition of germination [ 41 , 56 , 57 ] Lysozyme AMP NA NA Inhibition of hyphae metabolism [ 56 , 59 , 60 ] MASP-1/3 Complement system NA NA Recruit ficolin-3 to Af, activate complement and facilitate phagocytosis [ 28 ] MASP-2 Complement system NA ↓ in BAL To be studied [ 41 ] MBL Complement system Collectin rodA (conidia) Galactomannan (hyphae) ↑ in cornea ↑ in serum PRR binding to A. fumigatus and activating complement system through the lectin-pathway [ 36 , 48 , 128 ] PTX-3 APP NA ↑ in serum and BAL Opsonization promoting phagocytosis and killing by neutrophils [ 74 ] SAP APP NA = in serum ↑ in BAL Activates complement and enhances phagocytosis by neutrophils ...…”

Section: Introductionmentioning

confidence: 99%

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Humoral Immunity Against Aspergillus fumigatus

Dellière

Aimanianda

2023

Mycopathologia

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Aspergillus fumigatus is one the most ubiquitous airborne opportunistic human fungal pathogens. Understanding its interaction with host immune system, composed of cellular and humoral arm, is essential to explain the pathobiology of aspergillosis disease spectrum. While cellular immunity has been well studied, humoral immunity has been poorly acknowledge, although it plays a crucial role in bridging the fungus and immune cells. In this review, we have summarized available data on major players of humoral immunity against A. fumigatus and discussed how they may help to identify at-risk individuals, be used as diagnostic tools or promote alternative therapeutic strategies. Remaining challenges are highlighted and leads are given to guide future research to better grasp the complexity of humoral immune interaction with A. fumigatus.

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“…Dellière et al. dissect the regulation of alveolar humoral immune components during A. fumigatus infection ( 7 ). Using comparative proteomic analysis of the bronchoalveolar lavage (BAL) fluid collected from individuals infected or colonized with A. fumigatus , they identified several humoral immune components affected by A. fumigatus infection and colonization.…”

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Editorial: Interactions of Pentraxins and Complement in Infection, Inflammation, and Cancer

2022

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Complement is an important innate immune defense system that deploys efficient immunosurveillance and protection against pathogens and damaged host cells, and plays crucial roles in inflammation (1). The complement comprises over 50 effectors and receptors, which precisely balance activation, amplification and regulation of the complement proteolytic cascade organized by three canonical pathways (2). However, the complement often becomes host-offensive and leads to pathophysiological situations once disordered (3).Pentraxins are a superfamily of conserved multi-functional pattern recognition molecules (PRMs) with features of a cyclic multimeric structure and a conserved C-terminal pentraxin domain. As major members of the pentraxin family, C-reactive protein (CRP), serum-amyloid P component (SAP) and pentraxin 3 (PTX3) are synthesized mainly in response to inflammatory mediators and tissue injury, and function as essential constituents in innate immune defense against certain pathogenic intruders (4). Featuring antibody-like common properties, CRP, SAP and PTX3 often induce rapid and efficient immune effector mechanisms upon selective recognition of microbial moieties (5). Interestingly, recent data have shown that interaction between pentraxins and complement could build up comprehensive crosstalk with the three pathways of complement cascade activation (6).This Research Topic aims at collecting the latest findings to gain deeper insight into the functional roles of pentraxins in infection and regulation of inflammatory responses with a focus on the interaction with the complement system, thus directing potential therapeutic applications.Both pentraxins and complement play a non-redundant role in resistance to invasive pulmonary aspergillosis caused by Aspergillus fumigatus (A. fumigatus), but their crosstalk also has a synergic effect on their immune effector functions. Dellière et al. dissect the regulation of alveolar humoral immune components during A. fumigatus infection (7). Using comparative proteomic analysis of the bronchoalveolar lavage (BAL) fluid collected from individuals infected or colonized with A. fumigatus, they identified several humoral immune components affected by A. fumigatus infection and colonization. In agreement with previous findings, both complement factors and pentraxins were found as the major humoral immune components, for instance C1q, ficolin-2, MASP-2 and PTX3. These findings further substantiate an essential contribution of these PRMs and their synergistic effects via crosstalk during A. fumigatus-caused infection and inflammation. Further, the

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Proteomic Analysis of Humoral Immune Components in Bronchoalveolar Lavage of Patients Infected or Colonized by Aspergillus fumigatus

Cited by 7 publications

References 23 publications

Surfactant protein D inhibits growth, alters cell surface polysaccharide exposure and immune activation potential of Aspergillus fumigatus

Surfactant protein D inhibits growth, alters cell surface polysaccharide exposure and immune activation potential of Aspergillus fumigatus

Humoral Immunity Against Aspergillus fumigatus

Editorial: Interactions of Pentraxins and Complement in Infection, Inflammation, and Cancer

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