Proteomic Analysis of<i>Campylobacter jejuni</i>11168 Biofilms Reveals a Role for the Motility Complex in Biofilm Formation

Kalmokoff, Martin; Lanthier, Patricia; Tremblay, Tammy-Lynn; Foss, Mary; Lau, Peter C.; Sanders, Gregory A.; Austin, John W.; Kelly, John F.; Szymanski, Christine M.

doi:10.1128/jb.01975-05

Cited by 253 publications

(237 citation statements)

References 67 publications

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“…3B) revealed that the csrA mutant formed nearly 50% less biofilm than 81-176 (P ϭ 0.0001); however, the complemented mutant formed twice as much biofilm as the wild type. It has been demonstrated that flagellar function and responses to both general and oxidative stress are critical to biofilm formation (24,30,31,57,59). These results suggest that CsrA is an activator of biofilm formation, possibly via regulation of motility and oxidative stress responses in C. jejuni.…”

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confidence: 99%

Campylobacter jejuniCsrA Mediates Oxidative Stress Responses, Biofilm Formation, and Host Cell Invasion

2008

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Campylobacter jejuniCsrA Mediates Oxidative Stress Responses, Biofilm Formation, and Host Cell Invasion

2008

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“…It has been suggested that bacteria attached to broiler skin are less susceptible to detergents and other antibacterial treatments (15), as well as to oxidative stress (16). Sessile and planktonic bacteria have some physical changes in protein expression; in particular, increased expression of stress genes in sessile bacteria influences the persistence of attached bacteria (17).…”

Section: Discussionmentioning

confidence: 99%

Salmonella enterica Serovar Kentucky Flagella Are Required for Broiler Skin Adhesion and Caco-2 Cell Invasion

Salehi

Howe

Lawrence

et al. 2017

Appl Environ Microbiol

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Nontyphoidal Salmonella strains are the main source of pathogenic bacterial contamination in the poultry industry. Recently, Salmonella enterica serovar Kentucky has been recognized as the most prominent serovar on carcasses in poultry-processing plants. Previous studies showed that flagella are one of the main factors that contribute to bacterial attachment to broiler skin. However, the precise role of flagella and the mechanism of attachment are unknown. There are two different flagellar subunits (fliC and fljB) expressed alternatively in Salmonella enterica serovars using phase variation. Here, by making deletions in genes encoding flagellar structural subunits (flgK, fliC, and fljB), and flagellar motor (motA), we were able to differentiate the role of flagella and their rotary motion in the colonization of broiler skin and cellular attachment. Utilizing a broiler skin assay, we demonstrated that the presence of FliC is necessary for attachment to broiler skin. Expression of the alternative flagellar subunit FljB enables Salmonella motility, but this subunit is unable to mediate tight attachment. Deletion of the flgK gene prevents proper flagellar assembly, making Salmonella significantly less adherent to broiler skin than the wild type. S. Kentucky with deletions in all three structural genes, fliC, fljB, and flgK, as well as a flagellar motor mutant (motA), exhibited less adhesion and invasion of Caco-2 cells, while an fljB mutant was as adherent and invasive as the wild-type strain.IMPORTANCE In this work, we answered clearly the role of flagella in S. Kentucky attachment to the chicken skin and Caco-2 cells. We demonstrated that the presence of FliC is necessary for attachment to broiler skin. Expression of the alternative flagellar subunit FljB enables Salmonella motility, but this subunit is unable to mediate strong attachment. Deletion of the flgK gene prevents proper flagellar assembly, making Salmonella significantly less adherent to broiler skin than the wild type. S. Kentucky with deletions in all three structural genes, fliC, fljB, and flgK, as well as a flagellar motor mutant (motA), exhibited less adhesion and invasion of Caco-2 cells, while an fljB mutant was as adherent and invasive as the wild-type strain. We expect these results will contribute to the understanding of the mechanisms of Salmonella attachment to food products.

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“…FlaC shows significant homology to the FlaA and FlaB flagellins of the flagellar filament. However, flaC mutants are not defective for motility, as analyzed by in vitro methods (305,559). Instead, purified FlaC has been shown to bind to HEp-2 human epidermoid carcinoma cells, and a flaC mutant is unable to invade HEp-2 cells as well as a wild-type strain does (559).…”

Section: Adherence To Gastrointestinal Epitheliummentioning

confidence: 99%

Change Is Good: Variations in Common Biological Mechanisms in the Epsilonproteobacterial GeneraCampylobacterandHelicobacter

Gilbreath

Cody

Merrell

et al. 2011

Microbiol Mol Biol Rev

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SUMMARY Microbial evolution and subsequent species diversification enable bacterial organisms to perform common biological processes by a variety of means. The epsilonproteobacteria are a diverse class of prokaryotes that thrive in diverse habitats. Many of these environmental niches are labeled as extreme, whereas other niches include various sites within human, animal, and insect hosts. Some epsilonproteobacteria, such as Campylobacter jejuni and Helicobacter pylori , are common pathogens of humans that inhabit specific regions of the gastrointestinal tract. As such, the biological processes of pathogenic Campylobacter and Helicobacter spp. are often modeled after those of common enteric pathogens such as Salmonella spp. and Escherichia coli . While many exquisite biological mechanisms involving biochemical processes, genetic regulatory pathways, and pathogenesis of disease have been elucidated from studies of Salmonella spp. and E. coli , these paradigms often do not apply to the same processes in the epsilonproteobacteria. Instead, these bacteria often display extensive variation in common biological mechanisms relative to those of other prototypical bacteria. In this review, five biological processes of commonly studied model bacterial species are compared to those of the epsilonproteobacteria C. jejuni and H. pylori . Distinct differences in the processes of flagellar biosynthesis, DNA uptake and recombination, iron homeostasis, interaction with epithelial cells, and protein glycosylation are highlighted. Collectively, these studies support a broader view of the vast repertoire of biological mechanisms employed by bacteria and suggest that future studies of the epsilonproteobacteria will continue to provide novel and interesting information regarding prokaryotic cellular biology.

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Proteomic Analysis ofCampylobacter jejuni11168 Biofilms Reveals a Role for the Motility Complex in Biofilm Formation

Cited by 253 publications

References 67 publications

Campylobacter jejuniCsrA Mediates Oxidative Stress Responses, Biofilm Formation, and Host Cell Invasion

Campylobacter jejuniCsrA Mediates Oxidative Stress Responses, Biofilm Formation, and Host Cell Invasion

Salmonella enterica Serovar Kentucky Flagella Are Required for Broiler Skin Adhesion and Caco-2 Cell Invasion

Change Is Good: Variations in Common Biological Mechanisms in the Epsilonproteobacterial GeneraCampylobacterandHelicobacter

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