Proteomic analysis of intracellular protein corona of nanoparticles elucidates nano-trafficking network and nano-bio interactions

Qin, Mengmeng; Zhang, Jian; Li, Minghui; Yang, Dan; Liu, Dechun; Song, Santai; Fu, Jijun; Zhang, Hua; Dai, Wenbing; Wang, Xueqing; Wang, Yiguang; He, Bing; Zhang, Qiang

doi:10.7150/thno.38900

Cited by 58 publications

(54 citation statements)

References 53 publications

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“…The consumer products, large-scale manufacturers producing NMs, synthesizing, and research laboratories could be highlighted among the main sources leading to the release of NPs in the environment [26,27]. Despite intense interest in the problem of interaction between NPs and organisms and a relatively big amount of experimental data, the mechanisms of toxic action for NPs are still not fully clear [28][29][30]. Hence, the risk evaluation of nano-bio interaction, regulation, and development of the standards for safe production and utilization of NMs and NPs has become one of the high-priority problems in nanotechnology and nanotoxicology [31,32].…”

Section: Introductionmentioning

confidence: 99%

“…The difficulty of risk assessment in nanotoxicology is complicated by the variety of factors that could significantly change the properties and, therefore, the toxicity of NPs. Moreover, much uncertainty still exists about the relation between different parameters such as size, form, surface area, zeta potential of NPs, protein corona formation, and transformation of NPs inside of organisms and in the environment or combinations of these parameters and toxic properties of NPs [28,[33][34][35]. NPs appear in the aquatic environment by surface wash, atmospheric sedimentation, and direct spills occurring during their synthesis, application, and utilization.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of the Level and Mechanisms of Toxicity of Carbon Nanotubes, Carbon Nanofibers, and Silicon Nanotubes in Bioassay with Four Marine Microalgae

et al. 2020

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Nanoparticles (NPs) have various applications in medicine, cosmetics, optics, catalysis, environmental purification, and other areas nowadays. With an increasing annual production of NPs, the risks of their harmful influence to the environment and human health is rising. Currently, our knowledge about the mechanisms of interaction between NPs and living organisms is limited. Additionally, poor understanding of how physical and chemical characteristic and different conditions influence the toxicity of NPs restrict our attempts to develop the standards and regulations which might allow us to maintain safe living conditions. The marine species and their habitat environment are under continuous stress due to anthropogenic activities which result in the appearance of NPs in the aquatic environment. Our study aimed to evaluate and compare biochemical effects caused by the influence of different types of carbon nanotubes, carbon nanofibers, and silica nanotubes on four marine microalgae species. We evaluated the changes in growth-rate, esterase activity, membrane polarization, and size changes of microalgae cells using flow cytometry method. Our results demonstrated that toxic effects caused by the carbon nanotubes strongly correlated with the content of heavy metal impurities in the NPs. More hydrophobic carbon NPs with less ordered structure had a higher impact on the red microalgae P. purpureum because of higher adherence between the particles and mucous covering of the algae. Silica NPs caused significant inhibition of microalgae growth-rate predominantly produced by mechanical influence.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of the Level and Mechanisms of Toxicity of Carbon Nanotubes, Carbon Nanofibers, and Silicon Nanotubes in Bioassay with Four Marine Microalgae

et al. 2020

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“…It has been reported that the cellular internalization pathway may depend on the type of recipient cell, the origin of the exosomes, and/or the environment 39 in addition to the particle’s size, charge, and shape. Moreover, research indicates that the surfaces of nanoparticles adhere to various plasma proteins under physiological conditions, forming so-called “protein coronas” 40 , 41 . According to the manufacturer’s description, the carboxylate-modified nanoparticles used in the present study are expected to adhere to proteins and other biomolecules, but with extremely decreased avidity compared to those of hydrophobic particles.…”

Section: Discussionmentioning

confidence: 99%

Scavenger receptor MARCO contributes to cellular internalization of exosomes by dynamin-dependent endocytosis and macropinocytosis

Kanno

Hirano

Sakamoto

et al. 2020

Sci Rep

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Macrophage receptor with collagenous structure (MARCO) is a scavenger receptor class-A protein that is expressed on the cell surface of macrophages. MARCO mediates binding and ingestion of unopsonized environmental particles, including nano-sized materials. Exosomes are cell-derived, nano-sized vesicles (40–150 nm) that can contain lipids, RNA, DNA, and various proteins. Exosomes play an essential role in cell-to-cell communication via body fluids. However, mechanisms for the recognition and internalization of exosomes by recipient cells remain poorly characterized. In this study, cellular association of serum-derived fluorescent exosomes and 20-nm fluorescent nanoparticles (positive control) was compared between MARCO-expressing (CHO-MARCO) and control (CHO-CT) CHO-K1 cells to examine whether MARCO expression by recipient cells mediates the cellular uptake of exosomes and environmental nanoparticles. Fluorescence microscopic studies and quantitative analyses revealed that the cellular associations of both exosomes and 20-nm nanoparticles were greater in CHO-MARCO cells than in CHO-CT cells. Exosomes and nanoparticles colocalized with green fluorescent protein (GFP)-MARCO in cells transfected with GFP-MARCO-encoding constructs . Furthermore, inhibitory studies showed that actin reorganization and dynamin are involved in the MARCO-mediated cellular internalization of exosomes. These results indicated that MARCO plays a role in the uptake of exosomes.

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“…The consumer products, large-scale manufacturers producing NMs, synthesizing, and research laboratories could be highlighted among the main sources lead to the release of NPs in the environment [1,2]. Despite intense interest in the problem of interaction between NPs and organisms and a relatively big amounts of experimental data, the mechanisms of toxic action for NPs are still not fully clear [3][4][5]. Hence, the risk evaluation of nano-bio interaction, regulation, and development of the standards for safe production and utilization of NMs and NPs has become one of the highpriority problems in nanotechnology and nanotoxicology [6,7].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of the Level and Mechanisms of Toxicity of Carbon Nanotubes, Carbon Nanofibers, and Silicon Nanotubes in Bioassay with Four Marine Microalgae

Pikula¹,

Чайка²,

Zakharenko³

et al. 2020

Preprint

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Nanoparticles (NPs) have various applications in medicine, cosmetics, optics, catalysis, environmental purification, and other areas nowadays. With an increasing annual production of NPs, the risks of their harmful influence to the environment and human health is rising. Currently, our knowledge about the mechanisms of interaction between NPs and living organisms is limited. Additionally, poor understanding of how physical and chemical characteristic and different conditions influence the toxicity of NPs restrict our attempts to develop the standards and regulations which might allow us to maintain the safe living conditions. The marine species and their habitat environment are under continuous stress due to anthropogenic activities which result in the appearance of NPs in the aquatic environment. Our study aimed to evaluate and compare biochemical effects caused by the influence of different types of carbon nanotubes, carbon nanofibers, and silica nanotubes on four marine microalgae species. We have evaluated the changes in growth-rate, esterase activity, membrane polarization, and size changes of microalgae cells using flow cytometry method. Our results demonstrated that toxic effects caused by the carbon nanotubes strongly correlated with the content of heavy metal impurities in the NPs. More hydrophobic carbon NPs with less ordered structure had a higher impact on the red microalgae P. purpureum because of higher adherence between the particles and mucous covering of the algae; silica NPs caused significant inhibition of microalgae growth-rate predominantly produced by mechanical influence.

show abstract

Proteomic analysis of intracellular protein corona of nanoparticles elucidates nano-trafficking network and nano-bio interactions

Cited by 58 publications

References 53 publications

Comparison of the Level and Mechanisms of Toxicity of Carbon Nanotubes, Carbon Nanofibers, and Silicon Nanotubes in Bioassay with Four Marine Microalgae

Comparison of the Level and Mechanisms of Toxicity of Carbon Nanotubes, Carbon Nanofibers, and Silicon Nanotubes in Bioassay with Four Marine Microalgae

Scavenger receptor MARCO contributes to cellular internalization of exosomes by dynamin-dependent endocytosis and macropinocytosis

Comparison of the Level and Mechanisms of Toxicity of Carbon Nanotubes, Carbon Nanofibers, and Silicon Nanotubes in Bioassay with Four Marine Microalgae

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