Proteomic analysis of leaves from a diploid cybrid produced by protoplast fusion between Satsuma mandarin and pummelo

Wang, Lei; Pan, Zhiyong; Guo, Wen‐Wu

doi:10.1007/s11240-010-9764-y

Cited by 30 publications

(15 citation statements)

References 42 publications

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“…1 (G1) and mesophyll protoplasts of Hirado Buntan pummelo ( Citrus grandis (L.) Osbeck) (HBP). Their nuclear and cytoplasmic genome composition was verified [10] and further confirmed [13]. G1+HBP and HBP plants were grafted onto trifoliate orange seedling rootstock at the same time, and planted in the experimental field of the National Citrus Breeding Center at HuaZhong Agricultural University.…”

Section: Methodsmentioning

confidence: 87%

Comparative Transcript Profiling of a Male Sterile Cybrid Pummelo and Its Fertile Type Revealed Altered Gene Expression Related to Flower Development

Zheng

et al. 2012

PLoS ONE

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Male sterile and seedless characters are highly desired for citrus cultivar improvement. In our breeding program, a male sterile cybrid pummelo, which could be considered as a variant of male fertile pummelo, was produced by protoplast fusion. Herein, ecotopic stamen primordia initiation and development were detected in this male sterile cybrid pummelo. Histological studies revealed that the cybrid showed reduced petal development in size and width, and retarded stamen primordia development. Additionally, disorganized cell proliferation was also detected in stamen-like structures (fused to petals and/or carpel). To gain new insight into the underlying mechanism, we compared, by RNA-Seq analysis, the nuclear gene expression profiles of floral buds of the cybrid with that of fertile pummelo. Gene expression profiles which identified a large number of differentially expressed genes (DEGs) between the two lines were captured at both petal primordia and stamen primordia distinguishable stages. For example, nuclear genes involved in nucleic acid binding and response to hormone synthesis and metabolism, genes required for floral bud identification and expressed in particular floral whorls. Furthermore, in accordance with flower morphology of the cybrid, expression of PISTILLATA (PI) was reduced in stamen-like structures, even though it was restricted to correct floral whorls. Down-regulated expression of APETALA3 (AP3) coincided with that of PI. These finding indicated that, due to their whorl specific effects in flower development, citrus class-B MADS-box genes likely constituted ‘perfect targets’ for CMS retrograde signaling, and that dysfunctional mitochondria seemed to cause male sterile phenotype in the cybrid pummelo.

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Section: Methodsmentioning

confidence: 87%

Comparative Transcript Profiling of a Male Sterile Cybrid Pummelo and Its Fertile Type Revealed Altered Gene Expression Related to Flower Development

Zheng

et al. 2012

PLoS ONE

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“…Ribulose-bisphosphate carboxylase (band thirteen), fructose-bisphosphate aldolase (band fourteen) and 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase (band seven) are intracellular proteins (Wang et al 2010) found in all three sugar beet cell lines. These may have been released from cells that ruptured during the 4-day cultivation in liquid medium.…”

Section: Discussionmentioning

confidence: 99%

Morphological and proteomic analyses of sugar beet cultures and identifying putative markers for cell differentiation

Pavoković

Poljuha²,

Horvatić

et al. 2011

Plant Cell Tiss Organ Cult

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Normal (N), habituated nonorganogenic (HNO), and tumour (T) sugar beet cell lines were analysed in order to establish specific patterns of extracellular proteins and identify protein markers that might explain the distinct phenotypical characteristics. Electron microscopy showed that the ultrastructure of N cells corresponds to that of parenchyma cells, and that these cells contain plastids with large starch grains. HNO and T cells had enlarged, lobed nuclei with an increased number of nucleoli; the number of nuclei in HNO cells was greater than in T cells. The T plastids were elongated, with reduced thylakoids and abundant phytoferritin deposits, while HNO plastids were small and vacuolated, with an irregular, underdeveloped thylakoid system. The extracellular proteome of the cells was separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Greater differences in protein expression were observed between the HNO and N lines than between the T and N lines. Sixteen of the most prominent bands differentially expressed among the cell lines were cut out from the gel and analyzed by mass spectrometry. Cell wall-modifying enzymes were identified, including a peroxidase whose expression was twofold higher in N and T tissue than in HNO tissue; pectinesterase, which was expressed at a level threefold lower in the T line than in the other cell lines; and xyloglucan endotransglucosylase, which was expressed at a level sixfold higher in HNO and T tissue. Three proteins belonged to the chitinase gene family and their expression was higher in HNO and T tissue than in N tissue. The differential expression of these proteins suggests that these play a role in cell line-specific cell wall composition and cell-to-cell adhesion.

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“…Despite the need for new and improved cultivars the rate of development is slow due to long juvenile period, high heterozygosity and sexual incompatibility factors (Deng and Duan 2006). Gene transfer technologies such as Agrobacterium-mediated transformation Grosser 2009, 2010;Pasquali et al 2009;Ballester et al 2010;Duan et al 2010;Dutt et al 2011), particle gun bombardment and protoplast fusion (Wang et al 2010;Grosser and Gmitter 2011) offer an alternative approach for the transfer of gene traits into important germplasms. For this to be viable, a reliable and efficient tissue culture system needs to be established for the production of regenerated shoots.…”

Section: Introductionmentioning

confidence: 99%

Influence of surfactants on growth and regeneration from mature internodal stem segments of sweet orange (Citrus sinensis) cv. Hamlin

Curtis

Mirkov

2011

Plant Cell Tiss Organ Cult

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The development of a reliable shoot regeneration system for mature tissue of citrus is of major importance to accelerate the evaluation of commercial traits. Three non-ionic surfactants were evaluated independently in terms of their affects on the growth and regeneration of mature internodal stem segments of sweet orange cv. Hamlin in culture. Growth and shoot development of explants were influenced by type of surfactant added to the regeneration medium DBA3, its concentration and order of flush growth used for explant preparation.

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Proteomic analysis of leaves from a diploid cybrid produced by protoplast fusion between Satsuma mandarin and pummelo

Cited by 30 publications

References 42 publications

Comparative Transcript Profiling of a Male Sterile Cybrid Pummelo and Its Fertile Type Revealed Altered Gene Expression Related to Flower Development

Comparative Transcript Profiling of a Male Sterile Cybrid Pummelo and Its Fertile Type Revealed Altered Gene Expression Related to Flower Development

Morphological and proteomic analyses of sugar beet cultures and identifying putative markers for cell differentiation

Influence of surfactants on growth and regeneration from mature internodal stem segments of sweet orange (Citrus sinensis) cv. Hamlin

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