Proteomic analysis of macrophages: A new way to identify novel cell-surface antigens

Zhang, Lingbing; Lun, Yanni; Yan, Dongmei; Yu, Leyang; Ma, Wei; Du, Binghai; Zhu, X. D.

doi:10.1016/j.jim.2007.01.009

Cited by 29 publications

(24 citation statements)

References 23 publications

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“…However, classifying these cells on the basis of expression of CD11b and CD11c expression is an oversimplification, since it is clear that CD11b ϩ CD11c ϩ cells in the lungs of Mtbinfected mice could be activated alveolar M. In addition to lack of specificity of CD11c for DC, it is also not certain whether CD205 is a better lineage marker. Although CD205 is predominantly expressed by DC, it is also expressed by B cells and peritoneal macrophages (35)(36)(37). Importantly, inflammation may alter CD205 expression.…”

Section: Discussionmentioning

confidence: 99%

Tuberculosis Triggers a Tissue-Dependent Program of Differentiation and Acquisition of Effector Functions by Circulating Monocytes

Sköld

Behar

2008

The Journal of Immunology

121

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The origin and function of the different myeloid cell subsets that appear in the lung during pulmonary tuberculosis are unknown. Herein we show that adoptively transferred monocytes give rise to many of the macrophage and dendritic cell (DC) subsets that appear following aerosol infection with virulent Mycobacterium tuberculosis. Monocyte differentiation in infected peripheral tissue is surprisingly heterogeneous and results in the formation of five distinct myeloid subsets, including both classically activated macrophages, that produce inducible NO synthase via an IFN-γ-dependent mechanism, and DC. In contrast, monocytes recruited to draining pulmonary lymph nodes are functionally different and acquire a mature DC phenotype. Thus, while monocytes are recruited to the lungs of uninfected mice, their differentiation and acquisition of myeloid effector functions are dramatically altered in the presence of inflammation and bacteria and are dependent on tissue localization. Therefore, our results support a model in which recruited monocytes are well poised to influence multiple aspects of host immunity to infections in the lungs. This report provides the first direct evidence for monocyte differentiation into both the macrophage and DC lineages in vivo following infection with a live human pathogen.

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Section: Discussionmentioning

confidence: 99%

Tuberculosis Triggers a Tissue-Dependent Program of Differentiation and Acquisition of Effector Functions by Circulating Monocytes

Sköld

Behar

2008

The Journal of Immunology

121

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“…Proteome Software's SCAFFOLD 1.7. meta-analysis software, together with X! TANDEM (www.thegpm.org), was then used to statistically validate the peptide and protein findings of SEQUEST (60). Proteins were considered confidently identified when at least two different peptides were present at a probability of at least 95% and the protein probability was also 95% or higher.…”

Section: Methodsmentioning

confidence: 99%

“…As a negative control, the protein extracts were also incubated with the normal oligonucleotide (O4) and with beads only. The extracted peptides were analyzed by MS. A total of Ͼ50,000 MS/MS spectra were acquired by searching against the P. gingivalis proteome with Bioworks Browser 3.1 SR1 ALPHA7 software (60). More than 500 peptides were identified, with 35 peptides representing unique spectra for the 16 proteins listed in Table 4 (4 to 23% coverage).…”

mentioning

confidence: 99%

DNA Repair of 8-Oxo-7,8-Dihydroguanine Lesions in Porphyromonas gingivalis

Henry¹,

Sandberg

Zhang

et al. 2008

J Bacteriol

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The persistence of Porphyromonas gingivalis in the inflammatory environment of the periodontal pocket requires an ability to overcome oxidative stress. DNA damage is a major consequence of oxidative stress. Unlike the case for other organisms, our previous report suggests a role for a non-base excision repair mechanism for the removal of 8-oxo-7,8-dihydroguanine (8-oxo-G) in P. gingivalis. Because the uvrB gene is known to be important in nucleotide excision repair, the role of this gene in the repair of oxidative stressinduced DNA damage was investigated. A 3.1-kb fragment containing the uvrB gene was PCR amplified from the chromosomal DNA of P. gingivalis W83. This gene was insertionally inactivated using the ermF-ermAM antibiotic cassette and used to create a uvrB-deficient mutant by allelic exchange. When plated on brucella blood agar, the mutant strain, designated P. gingivalis FLL144, was similar in black pigmentation and beta-hemolysis to the parent strain. In addition, P. gingivalis FLL144 demonstrated no significant difference in growth rate, proteolytic activity, or sensitivity to hydrogen peroxide from that of the parent strain. However, in contrast to the wild type, P. gingivalis FLL144 was significantly sensitive to UV irradiation. The enzymatic removal of 8-oxo-G from duplex DNA was unaffected by the inactivation of the uvrB gene. DNA affinity fractionation identified unique proteins that preferentially bound to the oligonucleotide fragment carrying the 8-oxo-G lesion. Collectively, these results suggest that the repair of oxidative stress-induced DNA damage involving 8-oxo-G may occur by a still undescribed mechanism in P. gingivalis.

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“…In this respect, cell culture systems could represent a useful tool to partially overcome drawbacks of tissue analyses, allowing researchers to study single aspects of the atherosclerotic process in very controlled conditions. In the last years, studies on proteome (the intracellular proteins) and secretome (the proteins released into the cell culture medium) by 2DE and MS of ECs (Bruneel et al, 2003;Chen et al, 2007;Tunica et al, 2009), VSMCs (McGregor et al, 2001Dupont et al, 2005;Lee et al, 2006), and monocytes/macrophages (Dupont et al, 2004;Fach et al, 2004;Slomianny et al, 2006;Zhang et al, 2007;Zhao et al, 2009) have been performed. Moreover, in the attempt to help in elucidating the mechanisms of atherogenesis, several proteomic studies have been carried out on vascular cells cultured in different experimental conditions (table 3).…”

Section: Vascular Cell Proteomicsmentioning

confidence: 99%

Overview of Current Proteomic Approaches for Discovery of Vascular Biomarkers of Atherosclerosis

Antonio¹,

Zinellu²,

Formato³

2012

Proteomics - Human Diseases and Protein Functions

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Proteomic analysis of macrophages: A new way to identify novel cell-surface antigens

Cited by 29 publications

References 23 publications

Tuberculosis Triggers a Tissue-Dependent Program of Differentiation and Acquisition of Effector Functions by Circulating Monocytes

Tuberculosis Triggers a Tissue-Dependent Program of Differentiation and Acquisition of Effector Functions by Circulating Monocytes

DNA Repair of 8-Oxo-7,8-Dihydroguanine Lesions in Porphyromonas gingivalis

Overview of Current Proteomic Approaches for Discovery of Vascular Biomarkers of Atherosclerosis

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