Proteomic Analysis of Microvesicles Released by the Human Prostate Cancer Cell Line PC-3

Osteoblast-released Matrix Vesicles, Regulation of Activity and Composition by Sulfated and Non-sulfated Glycosaminoglycans

“…Furthermore, the existence of signal peptides was tested for all proteins identified in the MV fraction showing that 213 (68%) do not contain a signal peptide (supplemental Table 2D). This is in line with the idea that MV proteins are not released by the classical secretory pathway (48).…”

“…4, A and B) according to their biological processes, molecular functions, cellular components, and protein classes determined by the PANTHER classification tool (35). MVs showed no enrichment in plasma membrane proteins (3.4%), in agreement with the opinion that these vesicles do not originate to a large extent from the plasma membrane (48). Intracellular proteins (70.2%) were annotated to the cytosol and several organelles such as nucleus, ribosomes, and mitochondria.…”

Exosomes as Biomarker Enriched Microvesicles: Characterization of Exosomal Proteins Derived from a Panel of Prostate Cell Lines with Distinct AR Phenotypes

“…Reinforcing the conclusions of a recent published proteomic analysis of exosomes derived from the AR null PC3 cell line (63), this proteomic study also supports the premise that exosomes provide a pool of proteins which are enriched in biomarkers for potential use in PCa diagnosis via a relatively noninvasive diagnostic test. Our list consists of 50 candidate protein biomarkers which have been previously reported to have potential in diagnosis, prognosis, disease progression, efficacy and response to therapy for a variety of pathological diseases.…”

Prostasomes as a source of diagnostic biomarkers for prostate cancer

“…Findings that EVs from cancer cells have unique, cancer-specific contents (52) -together with the observation that prostasomes are present in both the blood and urine of PCa patients (53-56) -suggested the hypothesis that prostasomes may provide useful markers of PCa. This idea was supported further by early studies in which proteome and RNA profiles of EVs isolated from cultured prostate tumor cell lines and immortalized prostate epithelial cells were compared, identifying several potential candidate biomarker proteins for PCa (44,(57)(58)(59).…”