Proteomic Analysis of Mouse Brain Subjected to Spaceflight

Abstract

There is evidence that spaceflight poses acute and late risks to the central nervous system. To explore possible mechanisms, the proteomic changes following spaceflight in mouse brain were characterized. Space Shuttle Atlantis (STS-135) was launched from the Kennedy Space Center (KSC) on a 13-day mission. Within 3–5 h after landing, brain tissue was collected to evaluate protein expression profiles using quantitative proteomic analysis. Our results showed that there were 26 proteins that were significantly altered after spaceflight in the gray and/or white matter. While there was no overlap between the white and gray matter in terms of individual proteins, there was overlap in terms of function, synaptic plasticity, vesical activity, protein/organelle transport, and metabolism. Our data demonstrate that exposure to the spaceflight environment induces significant changes in protein expression related to neuronal structure and metabolic function. This might lead to a significant impact on brain structural and functional integrity that could affect the outcome of space missions.

Differential gene expression analysis of palbociclib-resistant TNBC via RNA-seq

“…These results are in line with previous studies showing that PI3K/AKT/mTOR activation mediates resistance to CDK4/6 inhibition in ER+ breast cancer [ 28 ]. Importantly, the PI3K/AKT/mTOR axis play a critical role in regulating cellular metabolism including lipid metabolism [ 29 , 30 ] and may be responsible for the deregulation of fatty acid and cholesterol observed in palbociclib-resistant TNBC cells. Similarly, we observed an increase in cyclin E in both TNBC cell models of acquired resistance to palbociclib.…”

Reptiles in Space Missions: Results and Perspectives

“…There are also data showing that space-relevant radiation may induce changes in neuronal functions. Additionally, exposure to spaceflights has a strong impact on metabolic and stress response [59].…”

Serum Metabolomics Associating With Circulating MicroRNA Profiles Reveal the Role of miR-383-5p in Rat Hippocampus Under Simulated Microgravity

“…focusing on multiple targets (mRNA, miRNA, protein and metabolic product, etc.). Conjoint analysis of the mass omics data to explore the complex mechanisms of adaptation syndrome can provide clues to study space medical problems (Nichols et al, 2006;Capulli et al, 2009;Chakraborty et al, 2014;Grimm et al, 2014;Rea et al, 2016;Mao et al, 2018). Among of these "Omics" methods, metabolomics or serum miRNA microarrays could monitor the influences of microgravity on the body at the small molecular levels and contribute to understanding the underlying molecular mechanisms of microgravity-induced disorders (Chen et al, 2016;Beheshti et al, 2018).…”

“…focusing on multiple targets (mRNA, miRNA, protein and metabolic product, etc.). Conjoint analysis of the mass omics data to explore the complex mechanisms of adaptation syndrome can provide clues to study space medical problems ( Nichols et al, 2006 ; Capulli et al, 2009 ; Chakraborty et al, 2014 ; Grimm et al, 2014 ; Rea et al, 2016 ; Mao et al, 2018 ).…”

“…However, there remains a lack of systematic investigation on the microgravity-induced effect, which is the key information needed to ultimately unfold the mechanism behind microgravity-induced physiological alternations. Recently, current omics tools such as genomic, transcriptomic, proteomic, metabolomic, and epigenetic approaches, allowing large-scale, high-throughput analyses for the detection, identification, and functional investigation, have gained a lot of attention ( Nichols et al, 2006 ; Rea et al, 2016 ; Schmidt et al, 2016 ; Kononikhin et al, 2017 ; Mao et al, 2018 ; Jin et al, 2019 ; Li et al, 2020 ). In particular, metabolomic and miRNAomic analyses in biofluids offer new methods for discovering biomarkers and molecular pathways underlying pathophysiological changes ( Chen et al, 2016 ).…”