Proteomic analysis of Mycobacterium tuberculosis isolates resistant to kanamycin and amikacin

Kumar, Bhavnesh; Sharma, Divakar; Sharma, Prashant; Katoch, Vishwa Mohan; Venkatesan, K; Bisht, Deepa

doi:10.1016/j.jprot.2013.08.025

Cited by 68 publications

(67 citation statements)

References 43 publications

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“…ACCEPTED MANUSCRIPT 4 the cell [3]. However, exact mechanisms of resistance to FQs are not fully known.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…Two-dimensional gel electrophoresis (2-DE) coupled with MALDI-TOF mass spectrometry (MS) and bioinformatics is a powerful and efficient tool for rapid identification of proteins. Comparative proteomic studies addressing whole cell proteins with first and second line drugs resistant isolates have been reported [4][5]. In spite of advancement in mycobacterial research, the knowledge about genes and their corresponding functional proteins under drug pressure with susceptible and resistant isolates with FQs has remained unaddressed.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…Isoelectric focusing (IEF) was carried out using the method of "in gel rehydration" with slight modifications [4,12]. Proteins were separated in second dimension on 12% SDS- .…”

Section: Two-dimensional Gel Electrophoresismentioning

confidence: 99%

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Proteomic analysis of ofloxacin-mono resistant Mycobacterium tuberculosis isolates

Lata

Sharma

Deo

et al. 2015

Journal of Proteomics

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“…ACCEPTED MANUSCRIPT 4 the cell [3]. However, exact mechanisms of resistance to FQs are not fully known.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Section: Accepted Manuscriptmentioning

confidence: 99%

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Proteomic analysis of ofloxacin-mono resistant Mycobacterium tuberculosis isolates

Lata

Sharma

Deo

et al. 2015

Journal of Proteomics

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“…In a complementary approach, systems-level analyses have been increasingly applied to investigate mycobacterial mechanisms of resistance to anti-TB drugs (Kumar et al 2013), as well as to elucidate the effect of common drugresistance mutations on bacillary physiology and cell function and, by implication, pathogenesis (Bisson et al 2012;du Preez and Loots 2012;Loots 2014). For example, proteomic profiling of M. tuberculosis isolates resistant to kanamycin and amikacin implicated putative iron homeostatic proteins as well as proteins of unknown function in the metabolic adaptation of mutants resistant to the major second-line aminoglycosides (Kumar et al 2013).…”

Section: The Metabolic Response To Drug Treatmentmentioning

confidence: 99%

“…For example, proteomic profiling of M. tuberculosis isolates resistant to kanamycin and amikacin implicated putative iron homeostatic proteins as well as proteins of unknown function in the metabolic adaptation of mutants resistant to the major second-line aminoglycosides (Kumar et al 2013). Moreover, comparative proteomics of clinical rifampicinresistant isolates has revealed differences in the expression profiles of specific rpoB mutants of the Mtb Haarlem and Beijing lineages compared with corresponding drug-susceptible counterparts (Bisson et al 2012).…”

Section: The Metabolic Response To Drug Treatmentmentioning

confidence: 99%

Mycobacterium tuberculosis Metabolism

Warner¹

2014

Cold Spring Harbor Perspectives in Medicine

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Metabolism underpins the physiology and pathogenesis of Mycobacterium tuberculosis. However, although experimental mycobacteriology has provided key insights into the metabolic pathways that are essential for survival and pathogenesis, determining the metabolic status of bacilli during different stages of infection and in different cellular compartments remains challenging. Recent advances-in particular, the development of systems biology tools such as metabolomics-have enabled key insights into the biochemical state of M. tuberculosis in experimental models of infection. In addition, their use to elucidate mechanisms of action of new and existing antituberculosis drugs is critical for the development of improved interventions to counter tuberculosis. This review provides a broad summary of mycobacterial metabolism, highlighting the adaptation of M. tuberculosis as specialist human pathogen, and discusses recent insights into the strategies used by the host and infecting bacillus to influence the outcomes of the host -pathogen interaction through modulation of metabolic functions.Ultimately, the process of pathogenicity itself will be describable in terms of the chemistry of the mycobacterial cell.-Wheeler and Ratledge (1994) T he term "metabolism" has a very wide purview. For bacteria, it is commonly used to describe the full set of complex and interconnected chemical transformations that enable individual cells to survive and replicate. In turn, these might be broadly divided into the anabolic pathways that consume energy in generating biomass and the energy-releasing catabolic reactions that channel organic building blocks into diverse cellular structures and pathways. Metabolism also includes pathways that are essential to the ability of bacteria to respond to dynamic environments and to maintain structural integrity in the face of fluctuating nutrient availability. Therefore, in an obligate pathogen such as Mycobacterium tuberculosis whose entire life cycle is driven in the context of human infection, metabolism necessarily underpins both physiology and pathogenesis (Rhee 2013).Owing to this dual role, the metabolism of M. tuberculosis has been the subject of intense research Beste and McFadden 2010). However, although experimental mycobacteriology-in particular, the use of genetic tools to disrupt enzymatic and regulatory functions-has provided critical insights into the metabolic pathways that are esEditors:

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An efficient and rapid method for enrichment of lipophilic proteins fromMycobacterium tuberculosisH37Rv for two-dimensional gel electrophoresis

Sharma

Bisht

2016

ELECTROPHORESIS

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Lipophilic proteome profiling is crucial because they have an anticipated role in biological processes and pathogenesis of Mycobacterium tuberculosis. These lipophilic proteins might be used as potential targets for the development of newer diagnostic markers and drug targets due to their association with membranes and drugs. We developed an efficient and rapid method to enrich the lipophilic proteins extraction from M. tuberculosis H37Rv for 2DE. In the extraction of lipophilic proteins, nonionic detergent (Triton X-100) was added in sonication buffer that augmented the solubilization of the proteins at the time of sonication. Enriched whole cell lysate was subjected to direct phase separation using Triton X-114, without the need for preisolation of membranes. In this study, we report that our optimized extraction buffer increased the lipophilic proteins extraction and their improved resolution on 2D gel up to two- to threefolds (quantitatively and qualitatively) as compared to standard extraction buffer. Some proteins were identified by MALDI-TOF/MS.

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Proteomic analysis of Mycobacterium tuberculosis isolates resistant to kanamycin and amikacin

Cited by 68 publications

References 43 publications

Proteomic analysis of ofloxacin-mono resistant Mycobacterium tuberculosis isolates

Proteomic analysis of ofloxacin-mono resistant Mycobacterium tuberculosis isolates

Mycobacterium tuberculosis Metabolism

An efficient and rapid method for enrichment of lipophilic proteins fromMycobacterium tuberculosisH37Rv for two-dimensional gel electrophoresis

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