Proteomic analysis of plasma from patients during an acute coronary syndrome

Mateos-Cáceres, Petra J.; García‐Méndez, Antonio; Farré, Antonio López; Macaya, Carlos; Núñez, A. Gil; Gómez, Juan Carlos Cárdenas; Alonso‐Orgaz, Sergio; Carrasco, Carolina; Burgos, M.; Andrés, Raimundo de; Granizo, Juan José; Farré, Jerónimo; Rico, Luís

doi:10.1016/j.jacc.2004.06.073

Cited by 136 publications

(140 citation statements)

References 25 publications

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“…Interestingly, apolipoprotein C-I and C-III appeared to be the first reported plasmatic biomarkers able to accurately distinguish between ischemic and hemorrhagic stroke within a small number of patients [72]. Proteomic analysis of plasma from patients suffering from acute myocardial infarction revealed also modifications of the protein pattern different from that of patients with unstable angina [73]. Proteomics has allowed the detection of drug-induced toxicity, and toxicoproteomics has developed [74].…”

Section: Clinical Applications -Biomarker Identificationmentioning

confidence: 99%

Recent advances in blood‐related proteomics

et al. 2005

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Service régional vaudois de transfusion sanguine, Lausanne, SwitzerlandBlood is divided in two compartments, namely, plasma and cells. The latter contain red blood cells, leukocytes, and platelets. From a descriptive medical discipline, hematology has evolved towards a pioneering discipline where molecular biology has permitted the development of prognostic and diagnostic indicators for disease. The recent advance in MS and protein separation now allows similar progress in the analysis of proteins. Proteomics offers great promise for the study of proteins in plasma/serum, indeed a number of proteomics databases for plasma/ serum have been established. This is a very complex body fluid containing lipids, carbohydrates, amino acids, vitamins, nucleic acids, hormones, and proteins. About 1500 different proteins have recently been identified, and a number of potential new markers of diseases have been characterized. Here, examples of the enormous promise of plasma/serum proteomic analysis for diagnostic/prognostic markers and information on disease mechanism are given. Within the blood are also a large number of different blood cell types that potentially hold similar information. Proteomics of red blood cells, until now, has not improved our knowledge of these cells, in contrast to the major progresses achieved while studying platelets and leukocytes. In the future, proteomics will change several aspects of hematology.

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Section: Clinical Applications -Biomarker Identificationmentioning

confidence: 99%

Recent advances in blood‐related proteomics

et al. 2005

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“…This fibrinogen γ chain isotype 1 has also been found increased in patients during an acute coronary syndrome. 11 In this regard, fibrinogen participates in platelet aggregation and inflammation. A different alternative splicing of fibrinogen γ chain messenger ribonucleic acid has recently been demonstrated that results in fibrinogen γ chain variants that alter fibrin formation and structure and could be associated with an increase of thrombotic risk.…”

Section: Discussionmentioning

confidence: 99%

Relationship between Vitamin D Binding Protein and Aspirin Resistance in Coronary Ischemic Patients: A Proteomic Study

et al. 2007

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Our aim was to analyze the plasma proteome in aspirin (acetylsalicylic acid [ASA])-sensitive and ASA-resistant coronary ischemic patients. Plasma from 19 ASA-sensitive and 19 ASA-resistant patients was analyzed. For the proteomic study, two-dimensional electrophoresis was performed. The expression of one isotype of the fibrinogen γ chain and three isotypes of haptoglobin was increased in ASA-resistant patients. Three vitamin D binding protein isotypes were increased in ASA-resistant patients. In vitro incubation of vitamin D binding protein (DBP) with blood from healthy volunteers reduced the inhibitory effect of ASA on thromboxane A2 production. DBP may be a new regulator of the inhibitory effect of ASA on platelets. Keywords: Aspirin (acetylsalicylic acid [ASA]) · plasma proteome · platelets · thromboxane A2

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“…Como se ha publicado previamente (135), las muestras se redujeron con ditioeritritol Mediante un pulsador se selecciona un determinado ion padre y sus iones fragmento, que son acelerados a un potencial mayor y separados en el segundo TOF.…”

Section: A10unclassified

“…Con el análisis en modo MS obtuvimos un espectro de masas denominado "huella peptídica" (Fig. 4.5), y algunos de los péptidos observados se analizaron en modo MS/MS, como se ha publicado anteriormente (135). El modo MS/MS obtiene espectros de fragmentación de las 3-5 masas mayoritarias dentro de cada una de las huellas peptídicas.…”

Section: A10unclassified

Efecto del alprostadil en los cultivos de músculo esquelético isquémico. Estudio proteómico comparativo frente a músculo sano

et al. 2014

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El presente trabajo reúne las condiciones de rigor y originalidad científica para ser presentada como tesis doctoral.Y para que conste a los efectos oportunos firmo la presente certificación en El presente trabajo reúne las condiciones de rigor y originalidad científica para ser presentada como tesis doctoral.Y para que conste a los efectos oportunos firmo la presente certificación en ABREVIATURAS• ADP: Adenosín Difosfato• AC: Actina• ATP: Adenosín Trifosfato• Arg: arginina• BCA: ácido bicinconínico• Bcl-2 :B-cell Lymphoma 2• BSA: albúmina del suero bovino• CE: Célula embrionaria• CHAPS: cholamidopropil-2 hidroxi-1 propanosulfato• CML: Célula madre linfoide• Dl: decilitros• DGºf: Variación de la energía libre de Gibbs estándar de formación• DHAP: Dihidroxiacetona-fosfato• EAP: Enfermedad arterial periférica• EDTA: ácido etilendiaminotetraacético• ESI : del inglés, ElectroSpray Ionization• FAD: Flavín adenina dinucleótido• FADD : factor associated death domain• F-6-P: Fructosa 6 fosfato• FT-MS : del inglés, Fourier Transform ion cyclotron• G3P: Gliceraldehido 3 fosfato• G6P: Gliceraldehido 6 fosfato 20• HDL: Lipoproteina de alta densidad• HEPES: ácido 4-(2-HidroxiEtil)-1-PiperacinaEtanosulfónico.• ITB: Indice tobillo brazo• IPG: del inglés Isoelectric Phocusing Gradient• LDH: lactato deshidrogenasa• LDL: Lipoproteina de baja densidad• Lys: lisina• MALDI : del ingles, Matrix-Assisted Laser Desorption/Ionization• MDH: malato deshidrogenasa• Mcl-1 : Mieloid cell leucemia-1• mg: milígramos• Mi: Miosina• ml: mililitros• mm: milímetros• mmHg: milímetros de Mercurio• mmol: milimol• MS: espectrometría de masas• NAD: Nicotinamida adenina dinucleotido• nm: nanometros• PaO2: presión parcial arterial de oxígeno• PAS: Presión arterial sistólica• PC: piruvato carboxilasa• PCR: Proteina C reactiva• PEP: fosfoenolpirúvico• PGE1: Prostaglandina E1 21• PGE2: Prostaglandina E2• pg: picogramos• PVDF :del inglés polyvinylidene difluoride• SDS-PAGE :del inglés sodium dodecyl sulfate polyacrylamide gel electrophoresis• seg: segundos• SFB: suero fetal bovino• SNC: Sistema Nervioso Central• TNF: Factor de necrosis tumoral• TOF : del inglés, time of flight• TPI: Triosafosfato isomerasa• UAD: Unidades Arbitrarias Densitométricas• μg: nanogramos• 2-DE: del inglés 2-dimensional electrophoresis Cada haz está conformado por miles de fibras, perfectamente separables porque cada una está envuelta por su propia capa de tejido conjuntivo llamada endomisio. El total de fibras que finalmente tendrá el músculo queda establecido ya a los 4 ó 5 meses de vida fetal. El grosor, en cambio, es al nacer la quinta parte de lo que tendrá de adulto. En éste es muy variable, según el músculo de donde se saque la muestra ( a través de una biopsia). En general el grosor va de 10 milimicrones a 100 milimicrones (1 micrón es una parte de mil). Su longitud suele ir a lo largo de todo el recorrido del músculo, alcanzando hasta 30 centímetros en algunos casos.En el interior de cada fibra se encuentran las miofibrillas segmentadas en una estructura histológica conocid...

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Proteomic analysis of plasma from patients during an acute coronary syndrome

Abstract: Plasma proteomic analysis makes it possible to develop a map of the protein isoforms that are expressed in plasma during an ACS.

Cited by 136 publications

References 25 publications

Recent advances in blood‐related proteomics

Recent advances in blood‐related proteomics

Relationship between Vitamin D Binding Protein and Aspirin Resistance in Coronary Ischemic Patients: A Proteomic Study

Efecto del alprostadil en los cultivos de músculo esquelético isquémico. Estudio proteómico comparativo frente a músculo sano

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