Proteomic analysis of plasma membrane and tonoplast from the leaves of mangrove plant<i>Avicennia officinalis</i>

Krishnamurthy, Pannaga; Tan, Xing Fei; Lim, Teck Kwang; Lim, Teck Guan; Kumar, Prakash P.; Loh, Chiang‐Shiong; Lin, Qingsong

doi:10.1002/pmic.201300527

Cited by 18 publications

(9 citation statements)

References 71 publications

(123 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Unfortunately, the outcomes of these projects were rather modest because adaptation to salt is a multigenic trait in plants ( Wang et al, 2012 ; Leonforte et al, 2013 ). A recent proteomic analysis of membrane proteins from the mangrove tree Avicennia officinalis helped to identify numerous membrane transporters and other membrane-integral proteins ( Krishnamurthy et al, 2014b ). However, on its own, this study was unable to provide a mechanistic basis for the salt tolerance exhibited by the mangroves, and it can only serve as a basis for future investigations into the mechanism.…”

Section: How Can the Salinity-adaptation Mechanism In The Date Palm Bmentioning

confidence: 99%

Salt tolerance research in date palm tree (Phoenix dactylifera L.), past, present, and future perspectives

2015

Self Cite

View full text Add to dashboard Cite

The date palm can adapt to extreme drought, to heat, and to relatively high levels of soil salinity. However, excessive amounts of salt due to irrigation with brackish water lead to a significant reduction in the productivity of the fruits as well as marked decrease in the viable numbers of the date palm trees. It is imperative that the nature of the existing salt-adaptation mechanism be understood in order to develop future date palm varieties that can tolerate excessive soil salinity. In this perspective article, several research strategies, obstacles, and precautions are discussed in light of recent advancements accomplished in this field and the properties of this species. In addition to a physiological characterization, we propose the use of a full range of OMICS technologies, coupled with reverse genetics approaches, aimed toward understanding the salt-adaption mechanism in the date palm. Information generated by these analyses should highlight transcriptional and posttranscriptional modifications controlling the salt-adaptation mechanisms. As an extremophile with a natural tolerance for a wide range of abiotic stresses, the date palm may represent a treasure trove of novel genetic resources for salinity tolerance.

show abstract

Section: How Can the Salinity-adaptation Mechanism In The Date Palm Bmentioning

confidence: 99%

Salt tolerance research in date palm tree (Phoenix dactylifera L.), past, present, and future perspectives

2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…In most true mangroves, the adaptations of seedlings to tolerate saline conditions have been widely studied [17]. However, the role of H 2 S in salinity tolerance improvement in mangroves is still mostly unknown.…”

Section: Introductionmentioning

confidence: 99%

Comparative Proteomic Analysis Reveals the Regulatory Effects of H2S on Salt Tolerance of Mangrove Plant Kandelia obovata

Liu

Shen

Simón

et al. 2019

IJMS

View full text Add to dashboard Cite

As a dominant mangrove species, Kandelia obovata is distributed in an intertidal marsh with an active H2S release. Whether H2S participates in the salt tolerance of mangrove plants is still ambiguous, although increasing evidence has demonstrated that H2S functions in plant responses to multiple abiotic stresses. In this study, NaHS was used as an H2S donor to investigate the regulatory mechanism of H2S on the salt tolerance of K. obovata seedlings by using a combined physiological and proteomic analysis. The results showed that the reduction in photosynthesis (Pn) caused by 400 mM of NaCl was recovered by the addition of NaHS (200 μM). Furthermore, the application of H2S enhanced the quantum efficiency of photosystem II (PSII) and the membrane lipid stability, implying that H2S is beneficial to the survival of K. obovata seedlings under high salinity. We further identified 37 differentially expressed proteins by proteomic approaches under salinity and NaHS treatments. Among them, the proteins that are related to photosynthesis, primary metabolism, stress response and hormone biosynthesis were primarily enriched. The physiological and proteomic results highlighted that exogenous H2S up-regulated photosynthesis and energy metabolism to help K. obovata to cope with high salinity. Specifically, H2S increased photosynthetic electron transfer, chlorophyll biosynthesis and carbon fixation in K. obovata leaves under salt stress. Furthermore, the abundances of other proteins related to the metabolic pathway, such as antioxidation (ascorbic acid peroxidase (APX), copper/zinc superoxide dismutase (CSD2), and pancreatic and duodenal homeobox 1 (PDX1)), protein synthesis (heat-shock protein (HSP), chaperonin family protein (Cpn) 20), nitrogen metabolism (glutamine synthetase 1 and 2 (GS2), GS1:1), glycolysis (phosphoglycerate kinase (PGK) and triosephosphate isomerase (TPI)), and the ascorbate–glutathione (AsA–GSH) cycle were increased by H2S under high salinity. These findings provide new insights into the roles of H2S in the adaptations of the K. obovata mangrove plant to high salinity environments.

show abstract

“…Among them, many of the proteins that are known to play a role in defence and stress responses in plants (e.g., stress proteins, HSPs, co-chaperones, dehydration-responsive/desiccation-related proteins, AAA-ATPase) were identified in the salt gland-rich tissues in this study ( Table 2 and S8 Table ). HSPs, for examples, which are one of those proteins commonly identified in this study, are members of the molecular chaperones that help to protect the plants against stress (e.g., salt stress) by refolding the proteins and maintaining their native conformation, thus preventing irreversible protein aggregation during adverse conditions [ 17 , 19 , 26 , 27 ]. Fructose-1,6-bisphosphate aldolase that had been identified and suggested to play a role in salt tolerance mechanisms that are common to both the glycophytes and mangrove plants [ 16 ] was also found in our salt gland-rich protein pools ( Table 2 ).…”

Section: Discussionmentioning

confidence: 99%

“…candel [ 17 ] and reported in the plasma membrane proteins (i.e., 14-3-3-like protein) of A . officinalis leaves [ 19 ] had also been identified herein in the salt gland-rich tissues ( Table 2 ).…”

Section: Discussionmentioning

confidence: 99%

“…These studies, however, focused on the non-secretors (i.e., Bruguiera gymnorhiza , Kandelia candel ) that do not have salt glands on their epidermal surfaces. Due to technical challenges faced in obtaining proteins directly from salt glands, a recent proteomic paper published by our group [ 19 ] focused on the plasma membrane and tonoplast proteins extracted from the leaves of A . officinalis .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Proteomic Characterisation of the Salt Gland-Enriched Tissues of the Mangrove Tree Species Avicennia officinalis

et al. 2015

Self Cite

View full text Add to dashboard Cite

Plant salt glands are nature’s desalination devices that harbour potentially useful information pertaining to salt and water transport during secretion. As part of the program toward deciphering secretion mechanisms in salt glands, we used shotgun proteomics to compare the protein profiles of salt gland-enriched (isolated epidermal peels) and salt gland-deprived (mesophyll) tissues of the mangrove species Avicennia officinalis. The purpose of the work is to identify proteins that are present in the salt gland-enriched tissues. An average of 2189 and 977 proteins were identified from the epidermal peel and mesophyll tissues, respectively. Among these, 2188 proteins were identified in salt gland-enriched tissues and a total of 1032 selected proteins were categorized by Gene Ontology (GO) analysis. This paper reports for the first time the proteomic analysis of salt gland-enriched tissues of a mangrove tree species. Candidate proteins that may play a role in the desalination process of the mangrove salt glands and their potential localization were identified. Information obtained from this study paves the way for future proteomic research aiming at elucidating the molecular mechanism underlying secretion in plant salt glands. The data have been deposited to the ProteomeXchange with identifier PXD000771.

show abstract

Proteomic analysis of plasma membrane and tonoplast from the leaves of mangrove plantAvicennia officinalis

Cited by 18 publications

References 71 publications

Salt tolerance research in date palm tree (Phoenix dactylifera L.), past, present, and future perspectives

Salt tolerance research in date palm tree (Phoenix dactylifera L.), past, present, and future perspectives

Comparative Proteomic Analysis Reveals the Regulatory Effects of H2S on Salt Tolerance of Mangrove Plant Kandelia obovata

Proteomic Characterisation of the Salt Gland-Enriched Tissues of the Mangrove Tree Species Avicennia officinalis

Contact Info

Product

Resources

About