Proteomic analysis of plumules and coleoptiles in maize between hybrids and their corresponding inbred lines

Jin, Xining; Fu, Zhiyuan; Ding, Dong; Li, Weihua; Liu, Zonghua; Hu, Yan; Tang, Jihua

doi:10.1007/s11738-013-1417-4

Cited by 11 publications

(13 citation statements)

References 52 publications

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“…Proteins, as performers of gene function, determine phenotypes, which can be thought of as snapshots of genome expression (Jin et al, 2014). Individual protein expression can be treated as inheritance of quantitative characteristics, even without prior information of protein identity, as most often relates to non-model species (Vasemägi and Primmer, 2005).…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of muscle between hybrid abalone and parental lines Haliotis gigantea Reeve and Haliotis discus hannai Ino

Luo

Wang

et al. 2015

Heredity

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To understand the potential molecular mechanism of heterosis, protein expression patterns were compared from hybrids of Haliotis gigantea (G) and Haliotis discus hannai (D) using two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight/time-of-flight analyses. Expression differences were observed in muscle samples from the four groups with 673 ± 21.0 stained spots for H. discus hannai ♀ × H. discus hannai ♂ (DD), 692 ± 25.6 for H. gigantea ♀ × H. gigantea ♂ (GG), 679 ± 16.2 for H. discus hannai ♀ × H. gigantea ♂ (DG) (F 1 hybrid) and 700 ± 19 for H. gigantea ♀ × H. discus hannai ♂ (GD) (F 1 hybrid). Different 2-DE image muscle protein spots had a mirrored relationship between purebreds and the F 1 hybrid, suggesting that all stained spots in F 1 hybrid muscle were on 2-DEs from parents. DD and DG clustered together first, and then clustered with GD, whereas the distance of DD and GG was maximal according to hierarchical cluster analysis. We identified 136 differentially expressed protein spots involved in major biological processes, including energy metabolism and stress response. Most energy metabolism proteins were additive, and stress-induced proteins displayed additivity or overdominance. In these 136 identified protein spots, hybrid offspring with additivity or over-dominance accounted for 68.38%. Data show that a proteomic approach can provide functional prediction of abalone interspecific hybridization.

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Section: Introductionmentioning

confidence: 99%

Proteomic analysis of muscle between hybrid abalone and parental lines Haliotis gigantea Reeve and Haliotis discus hannai Ino

Luo

Wang

et al. 2015

Heredity

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“…In some studies, it was observed that a greater speed of germination and vigorous growth of seedlings were associated with greater metabolic activity of RNA, proteins, and DNA in the embryos (Fu and Dooner, 2002;Meyer et al, 2007;Guo et al, 2013;Jin et al, 2014). The authors also observed that in the hybrid embryo, the metabolism of proteins and lipids was greater than in the lines, favoring the growth of the embryonic axis and greater seed germination.…”

Section: Introductionmentioning

confidence: 99%

Relationship among physiological quality, heterosis, and amylase gene expression in maize seeds

et al. 2015

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ABSTRACT. In this study, we analyzed heterosis, amylase enzyme gene expression, and the physiological quality of maize seeds with different genotypes and sizes, which were subjected to aging and not subjected to aging. We used seeds from 2 maize lines that differed with regard to physiological quality, the hybrid, and the reciprocal hybrid; they were classified into 2 sizes and were subjected to aging and not subjected to aging. Physiological quality was assessed by performing tests for germination, emergence, emergence speed index, and artificial aging. Expressions of the genes alpha amylase B73, alpha amylase (LOC542522), isoamylase mRNA clone 353244, and the endogenous controls ubiquitin and alcohol dehydrogenase in the seeds were studied using quantitative real-time-polymerase chain reaction. We observed heterosis for seed quality and for expression of amylase genes in the G.E. Oliveira et al. 8624©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 8623-8633 (2015) genotypes studied. We found no difference in seed quality between large and small seeds.

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“…An increase in seed storage temperature results in rapid resumption of several metabolic activities such as respiration, enzymatic activity, RNA, and protein synthesis (Yang et al 2007;He et al 2011;Kolodziejczyk et al 2016). For germination, energy is primarily supplemented by glycolysis and fermentation followed by the tricarboxylic acid (TCA) cycle, gluconeogenesis and glyoxylate cycle, and pentose phosphate pathway (Jin et al 2014;He and Yang 2013). The abundance of enolase (spot 140), an enzyme of the glycolysis/ gluconeogenesis pathway, and pyruvate dehydrogenase (spot 45), an enzyme linking glycolysis to the TCA cycle, was highest after storage at 3°C, and lowered with decreasing of storage temperature.…”

Section: Proteins Involved In Metabolism Processesmentioning

confidence: 99%

Proteomic analysis of black poplar (Populus nigra L.) seed storability

Pawłowski

Klupczyńska

Leśniewska

et al. 2019

Annals of Forest Science

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& Key message Adequate storability of black poplar (Populus nigra L.) seeds at temperatures below 0°C was associated with preservation of proteins of energy and carbohydrate metabolism, protein turnover, and proteins maintaining longterm stability of dehydrated tissue. & Context Understanding seed storability is a key factor for effective seed preservation and conservation. Black poplar is an endangered tree species and its seed loses rapidly viability during storage. & Aims The aim of this study was to determine, and functionally characterise, the proteins associated with storability of black poplar seeds. & Methods Dried seeds (7.1% MC) were stored at 3°C, − 3°C, − 20°C, and − 196°C, for a period of 12 and 24 months. Proteins were extracted and separated according to their isoelectric point (pI) and mass using 2-dimensional electrophoresis. Proteins that varied in abundance for temperature and time of storage were identified by mass spectrometry. & Results A germination test showed that seeds remained viable at − 3, − 20, and − 196°C. Storage at 3°C caused loss of seed viability. This loss in seed vigour was related to the largest changes in protein abundance. As storage temperature decreased, a smaller number of proteins displayed changed abundance. & Conclusion Good storability of black poplar seeds under freezing conditions may be associated with the following: an inhibition of synthesis of energy and carbohydrate metabolism and protein turnover proteins, LEA proteins that maintain long-term stability of dehydrated tissue, GDSL esterases/lipases that inhibit hydrolysing activity, and by chaperonins that protect protein functionality. Storage conditions influence the preservation of protein function that governs seed viability.

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Proteomic analysis of plumules and coleoptiles in maize between hybrids and their corresponding inbred lines

Cited by 11 publications

References 52 publications

Proteomic analysis of muscle between hybrid abalone and parental lines Haliotis gigantea Reeve and Haliotis discus hannai Ino

Proteomic analysis of muscle between hybrid abalone and parental lines Haliotis gigantea Reeve and Haliotis discus hannai Ino

Relationship among physiological quality, heterosis, and amylase gene expression in maize seeds

Proteomic analysis of black poplar (Populus nigra L.) seed storability

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